Cellular heterogeneity landscape in laryngeal squamous cell carcinoma

Abstract

Laryngeal squamous cell carcinoma (LSCC) is a highly malignant tumor originated from respiratory system. Although there have been many improvements in therapy until now, reducing the high mortality remains difficult. Understanding the cellular heterogeneity of LSCC could contribute to improve this problem. Single-cell RNA sequencing was applied to dissect the cell composition and molecular characteristics of LSCC tissues. Immunohistochemistry staining of the LSCC tissues was performed to identify the spatial location of tumor cells. Survival analysis of marker genes was executed in The Cancer Genome Atlas to verify the correlation between each cell clusters and patients' prognosis. The LSCC tissue cells were finely grouped into various clusters, including tumor cells, immune cells, epithelial cells, fibroblasts and endothelial cells. Notably, in tumor cells, keratinocyte-like cells were in the core of tumor while malignant proliferating cells were located at the tumor edge. The malignant proliferating cells were correlated with poor prognosis. In summary, this is the first study to delineate a landscape of the LSCC intratumor heterogeneity. Our work might help researchers have a better understanding for tumor progression.

Characteristics of B lymphocyte infiltration in HPV+ head and neck squamous cell carcinoma

“…Then immune cells and tumor cells were separately grouped and annotated based on the cell signature marker 17 . Data processing and analysis were performed as previously described 18 …”

An integrative microenvironment approach for laryngeal carcinoma: the role of immune/methylation/autophagy signatures on disease clinical prognosis and single-cell genotypes

“…The study of tumor cell heterogeneity allows for better understandings of tumor progression [101]. Laryngeal cancer single-cell data was clustered by pRS genes and fibroblasts associated with poor prognosis were postulated in this research.…”

“…Laryngeal cancer single-cell data was clustered by pRS genes and fibroblasts associated with poor prognosis were postulated in this research. Malignant proliferating cells and fibroblasts share the tumor margin in laryngeal cancer [101]. Numerous previous studies have confirmed that fibroblasts are one of the major components of TME and can establish dangerous associations with other TME cells, thereby creating a tumor-supporting environment in laryngeal cancer [102].…”

The Role of IL-9 Polymorphisms and Serum IL-9 Levels in Carcinogenesis and Survival Rate for Laryngeal Squamous Cell Carcinoma

“…However, it has been clarified that environmental carcinogens can induce damage of DNA and cause genomic irregularity [35][36][37][38][39][40]. Knowing that most LSCC patients are heavy smokers and alcohol consumers, studies analysing the molecular landscape and heterogeneity of LSCC, based on genetic alterations, could contribute to better understanding of the pathogenesis of this disease [7].…”

“…Additionally, the lack of screening programs (endoscopic laryngeal examination performed by an otorhinolaryngologist) results in a delayed diagnosis of LSCC [6]. Hence, a better understanding of LSCC cellular heterogeneity could contribute to improving early diagnostics and survival rates of LSCC patients [7]. To find new blood-or 2 of 16 tissue-based biomarkers and to assess their association with the morphological and clinical manifestations of LSCC, recent research has focused on the role of genetic susceptibility factors as single nucleotide polymorphisms in carcinogenesis [8][9][10].…”