Cellular Identification and In Silico Characterization of Protein Phosphatase 2C (PP2C) of Cryptosporidium parvum

Protein Phosphatase PP2C Identification in Entamoeba spp

“…In the present study, a 45.2 kDa molecule was recognized by the anti- Lmx PP2C antibody in the total extract, membrane fraction, and cytosolic fraction of E. histolytica , E. invadens , and E. dispar . PP2Cs of the same molecular weight have been identified in other microorganisms [ 30 , 51 , 52 ]. The location of PP2C was confirmed by immunofluorescence, revealing membrane and cytosolic expression in all Entamoeba species.…”

“…In Plasmodium , a PP2C regulates the development of the parasite [ 55 ]. Recently, identification has been made by immunofluorescence by crossreaction with an antibody of PP2C from L. major in Cryptosporidium parvum (CpPP2C), observed in the nucleus of oocysts and at the apical end of the sporozoite body [ 51 ].…”

“…Additionally, a 75 kDa cytosolic protein was presently identified in its active form on E. invadens , being equally expressed in trophozoites and cysts. Our recent identification and localization of PP2C in different protozoan parasites, such as Leishmania and Cryptosporidium , open a new perspective on the participation of these enzymes in essential functions of these parasites [ 30 , 51 , 52 ]. The encysting process of E. invadens was examined in the current contribution, finding that the PP2C phosphatase is in trophozoites of a molecular size of 45.2 kDa as well as cysts (at different maturation times) of a molecular size of approximately 75 kDa, primarily in the cytosolic fraction.…”