Cellular Identification of a Novel Uncultured Marine Stramenopile (MAST-12 Clade) Small-Subunit rRNA Gene Sequence from a Norwegian Estuary by Use of Fluorescence In Situ Hybridization-Scanning Electron Microscopy

Kołodziej, Karolina; Stoeck, Thorsten

doi:10.1128/aem.02158-06

Cited by 41 publications

(22 citation statements)

References 38 publications

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“…One example of an innovative approach is the combination of FISH and electron microscopy for obtaining ultrastructural details of uncultured protists (Kolodziej and Stoeck 2007;. Other approaches are being developed to obtain grazing rates of uncultured heterotrophic flagellates in the complex scenario of natural assemblages.…”

Section: Linking Diversity and Function For Uncultured Heterotrophic mentioning

confidence: 99%

Protistan Grazing on Marine Bacterioplankton

Jürgens

Massana

2008

Microbial Ecology of the Oceans

137

105

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Section: Linking Diversity and Function For Uncultured Heterotrophic mentioning

confidence: 99%

Protistan Grazing on Marine Bacterioplankton

Jürgens

Massana

2008

Microbial Ecology of the Oceans

137

105

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“…In addition numerous novel clades of small and presumably heterotrophic stramenopiles (MASTs; MArine STramenopiles) have been detected in 18S rRNA gene libraries from various marine environments (Massana et al. , 2004; Kolodziej and Stoeck, 2007; Massana and Pedrós‐Alió, 2008). Heterotrophic stramenopile flagellates are common in freshwater, brackish waters, soil and marine habitats (Verhagen et al.…”

Section: Introductionmentioning

confidence: 99%

Characterization of halotolerant Bicosoecida and Placididea (Stramenopila) that are distinct from marine forms, and the phylogenetic pattern of salinity preference in heterotrophic stramenopiles

Park

Simpson

2010

Environmental Microbiology

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Recent culture-based studies demonstrate the distinctiveness of the microbial eukaryote biota of very hypersaline environments. In contrast, microscopy-based faunistic studies suggest that the biota of habitats of more moderate hypersalinity (60-150 per thousand) overlaps substantially with that of marine environments, but this has barely been studied with modern techniques. To investigate the diversity and salinity tolerance range of these organisms, eight cultures of heterotrophic stramenopiles were established from (or from nearby) moderately hypersaline locations. These isolates represent five independent groups; Groups A, B and C are bicosoecids; Groups D and E belong to Placididea. One isolate (Group A) is a strain of the widespread marine species Cafeteria roenbergensis, and cannot grow above 100 per thousand salinity. The other isolates - Groups B-E - can all grow at 150-175 per thousand salinities and are probably moderate halophiles. Groups B-E all represent previously unsequenced species or even genera, although Group B is the sister group of the borderline extreme halophile Halocafeteria. The high level of novelty en countered suggests that moderately hypersaline environments may harbour a heterotrophic stramenopile biota distinct from that of marine environments. Interestingly, our new isolates are all most closely related to marine or halophilic forms, and our phylogenies show large clades defined by saline/non-saline habitats within bicosoecids, placidomonads and related lineages. In particular, most freshwater/soil bicosoecids form one well-supported clade. The sole major exception is Bicosoeca, which is intermixed with marine environmental sequences originally referred to as 'MAST-13', which are from brackish water, not typical seawater. It seems that the freshwater/marine barrier has been crossed very few times in the evolutionary history of these heterotrophic stramenopile flagellates.

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“…2002) and scanning electron microscopy (Stoeck et al . 2003; Kolodziej & Stoeck 2007), are also available but time‐consuming and not widely applied up to now.…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of unculturable Karyorelictea (Alveolata, Ciliophora)

Andreoli¹,

Mangini²,

Ferrantini³

et al. 2009

Zoologica Scripta

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Karyorelictea is a class of traditionally unculturable ciliates characterized by a non-dividing macronucleus. Their classification has been recently revised and morphological redescription of many species has been performed as well. On the contrary, molecular data of karyorelictean ciliates are largely underrepresented in public databases. In the present article we resumed and improved a method to characterize 18S rRNA gene sequences through direct amplification and sequencing of single cells. Using this approach, we characterized 12 different karyorelictean molecular operational taxonomic units (MOTUs: nine trachelocercids, one Geleia, one Remanella and one Loxodes), most of which were also photo-recorded. These molecular data were used to reconstruct phylogenetic relationships among the three orders (Protostomatida, Loxodida and Protoheterotrichida) in which the class is traditionally subdivided. The most supported tree topology shows an association between orders Loxodida (Loxodes, Remanella) and Protoheterotrichida (Geleia), in contrast with previous works associating orders Loxodida and Protostomatida (trachelocercids) on a morphological basis.

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Cellular Identification of a Novel Uncultured Marine Stramenopile (MAST-12 Clade) Small-Subunit rRNA Gene Sequence from a Norwegian Estuary by Use of Fluorescence In Situ Hybridization-Scanning Electron Microscopy

Cited by 41 publications

References 38 publications

Protistan Grazing on Marine Bacterioplankton

Protistan Grazing on Marine Bacterioplankton

Characterization of halotolerant Bicosoecida and Placididea (Stramenopila) that are distinct from marine forms, and the phylogenetic pattern of salinity preference in heterotrophic stramenopiles

Molecular phylogeny of unculturable Karyorelictea (Alveolata, Ciliophora)

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