Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Sood, Sunandan; Rishi, Praveen; Vohra, Harpreet; Sharma, Shaveta; Ganguly, Nirmal Kumar

doi:10.1099/jmm.0.46042-0

Cited by 19 publications

(11 citation statements)

References 32 publications

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“…Antigen-specific lymphocyte proliferation assay measures cell-mediated immune response against Salmonella in poultry 70 and mice. 71 OmpA-vaccinated murine splenocytes co-cultured with APCs enhanced T-cell proliferation, Th1 polarization and IFN-γ production. 72 Nanosized spherical particles enhance strong Th1 and Th2 immune responses.…”

Section: Discussionmentioning

confidence: 90%

Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens

Sankar¹,

Markazi²,

Dhakal³

et al. 2020

IJN

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Purpose: Salmonellosis in poultry is a serious economic burden. A major concern is the public health hazard caused by consumption of Salmonella-contaminated poultry products. Currently used Salmonella vaccines are ineffective in combating poultry Salmonellosis warranting the need of a potent vaccine, especially an oral vaccine that can elicit robust local intestinal immunity. Materials and Methods: A Salmonella subunit chitosan nanoparticles (NPs)-based vaccine was prepared that contained immunogenic outer membrane proteins (OMPs) and-flagellin (F) protein (OMPs-F-CS NPs). OMPs-F-CS NPs were administered as an oral vaccine in layer chickens and the resultant humoral and cell-mediated immune responses and localization of NPs were examined using standard detection methods. Results: We demonstrated targeting of surface F-protein coated chitosan NPs to immune cells when delivered orally to layer chickens, the particles were localized in ileal Peyer's patches. The OMPs-F-CS NPs vaccinated layer chickens had significantly higher OMPsspecific mucosal IgA production and lymphocyte proliferation response. The candidate vaccine increased the expression of toll-like receptor (TLR)-2, TLR-4, IFN-γ, TGF-ß and IL-4 mRNA expression in chicken cecal tonsils. Conclusion: Our study demonstrated that the chitosan-based oral Salmonella nanovaccine targets immune cells of chickens and induced antigen-specific B and T cell responses. This candidate oral Salmonella nanovaccine has the potential to mitigate Salmonellosis in poultry.

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Section: Discussionmentioning

confidence: 90%

Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens

Sankar¹,

Markazi²,

Dhakal³

et al. 2020

IJN

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“…Three bacterial strains i.e Salmonella typhimurium NCTC74, Yersinia enterocolitica ATCC 23715 as well as Staphylococccus aureus ATCC 9144 were selected to assess the antibacterial activity of cryptdin-2. Salmonella is responsible for a variety of infections in humans and domestic animals ranging from gastroenteritis caused by S. typhimurium to severe systemic disease caused by S. typhi [12,13]. Although Y. enterocolitica commonly causes intestinal infection in the form of mesenteric lymphadenitis and enterocolitis, the clinical picture is nevertheless quite variable [14].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Paneth Cells Derived Cryptdin-2 Against Selected Pathogens

Preet¹,

Rishi²

2010

Am. J. Biomed. Sci.

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As cationic antimicrobial peptides are major players of the innate immune response with broad antimicrobial activities, we evaluated the activity of Paneth cells derived cryptdin-2 against selected bacterial pathogens i.e Yersinia enterocolitica, Salmonella typhimurium (Gram negative) and Staphylococcus aureus (Gram positive). Cryptdin(s) preparations were obtained from crypt homogenates as well as from Paneth cells and analyzed using SDS-PAGE. A peptide band corresponding to 3.5 KDa (appx.) was eluted and purity was confirmed using HPLC. It was identified as cryptdin-2 according to its amino acid sequence. The peptide was found to have antimicrobial activity against the selected pathogens as indicated by radial diffusion assay as well as standard colony count technique. However, the activity was found to be the highest for S. aureus. This study thus highlights an important role of cryptdin-2 during host-pathogen interactions and indicates its potential as an inhibitory agent against the enteric infections.

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“…In a study by Srinivasan et al, [19] doses of 10 7 -10 9 were able to increase the level of CD4 + T cell clonal expansion and the expression of CD11a. In addition, Sood et al [25] reported that the higher number of CD4 + T cells in the bone marrow is linear, as evidenced by the increased ratio of CD4 + T cells/CD8 + T seen in Peyer's patches and the spleens of mice treated with exposure to the S. typhi outer membrane protein (OMP). Furthermore, the authors noted a massive expansion of CD4 + T cells relative to the number found in the bone marrow after S. typhi exposure, indicating that this bacteria is capable of causing systemic infection that can reach the bone marrow.…”

Section: Resultsmentioning

confidence: 99%

Splenectomy and Salmonella Typhi Exposure Lead to Quantitative Alterations of Bone Marrow B220+ Cells and CD4+ T Cells on BALB/c Mice

Rifa’i¹

2013

Turk J Immunol

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Amaç: Bu çalışmada splenektomi yapılan ve Salmonella typhi'ye (S. typhi) maruz bırakılan BALB/c farelerde kemik iliği B220 + hücreleri ve CD4 + T hücrelerinin rölatif yoğunluğundaki kantitatif değişiklikler incelendi. Gereç ve yöntemler:Ketamin anestezisi altında splenektomi yapıldı (65 mg/kg BW). Splenektomiden iki hafta sonra akut doz enjeksiyonu ile S. typhi maruziyeti yapıldı (10 9 hücre/mL) ve doz, ilk dozdan iki hafta sonra tekrarlandı. Kemik iliği B220 + hücreleri ve CD4 + T hücrelerinin rölatif yoğunluğu, BD FACSCalibur™ akım sitometri ile belirlendi. İki yönlü ANOVA ve Tukey HSD testi ile istatistiksel analiz yapıldı. P<0.05 değerleri anlamlı kabul edildi. Bulgular:Tedavilerinin tümü, gözlenen değişkenler üzerinde anlamlı etkilere sahipti. Splenektomi,B220+ hücrelerin oranınını splenektomi yapılmayan farelerdeki %17.34 oranından %5.51'e anlamlı olarak düşürdü. Salmonella typhi maruziyeti tek başına B220 + hücrelerinin rölatif yoğunluğunu anlamlı düzeyde değiştir-medi (%17.92). Splenektomi yapılması ve S.typhi ile karşılaşma B220+ hücreleri %5.9'a düşürdü. CD4 + T hücreleri tüm tedavilere karşı farklı yanıtlar verdi. Kontrollere kıyasla (%0.28), splenektomi CD4 + T hücrelerinin rölatif yoğunluğunu anlamlı düzeyde %0.43'e kadar artırdı. Salmonella typhi maruziyeti tek başına ve splenektomi ile birlikte, CD4 + T hücrelerinin rölatif yoğunluğunu sırası ile %0.55 ve %0.50'ye kadar anlamlı düzeyde artırdı.Sonuç: Bu bulgular, S. typhi maruziyetine kıyasla, splenektominin B220 + hücrelerinin rölatif yoğunluğunu etkileyen başlıca etmen olup, S. typhi maruziyeti CD4 + T hücrelerinin rölatif yoğunluğunu artıran ana etmendir. Bu fenomen, S. typhi maruziyeti nedeni ile ortaya çıkan patolojik durumu yansıtmaktadır.Anahtar sözcükler: B220 + hücreleri, CD4 + T hücreleri, Salmonella typhi, splenektomi. Objectives: This study aims to determine the quantitative alterations of relative density of bone marrow B220 + cells and CD4 + T cells in BALB/c mice which underwent splenectomy and exposed to Salmonella typhi (S. typhi). Materials and methods: Splenectomy was performed under ketamine anesthesia (65 mg/kg BW). Exposure of S. typhi was performed by injection of acute doses (109 cells/mL) at two weeks after splenectomy and repeated at two weeks after the initial dose. The relative density of bone marrow B220 + cells and CD4 + T cells were quantified by BD FACSCalibur TM flow cytometer. Statistical analysis was performed using two-way ANOVA and Tukey HSD test. A p value of <0.05 was considered significant. Conclusion:These results indicated that splenectomy was the leading factor affecting the relative density of B220 + cells, rather than S. typhi exposure, whereas Salmonella typhi exposure was the main factor which increased the relative density of CD4 + T cells. This phenomenon also reflects the pathological condition due to S. typhi exposure.

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Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Cited by 19 publications

References 32 publications

<p>Oral Deliverable Mucoadhesive Chitosan-<em>Salmonella</em> Subunit Nanovaccine for Layer Chickens</p>

<p>Oral Deliverable Mucoadhesive Chitosan-<em>Salmonella</em> Subunit Nanovaccine for Layer Chickens</p>

Antimicrobial Activity of Paneth Cells Derived Cryptdin-2 Against Selected Pathogens

Splenectomy and Salmonella Typhi Exposure Lead to Quantitative Alterations of Bone Marrow B220+ Cells and CD4+ T Cells on BALB/c Mice

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