Cellular immunity in ASFV responses

Takamatsu, H.; Denyer, Mick; Lacasta, Anna; Stirling, Catrina; Argilaguet, Jordi; Netherton, Christopher L.; Oura, C.A.L.; Martins, Carlos; Rodrı́guez, Fernando

doi:10.1016/j.virusres.2012.11.009

Cited by 138 publications

(145 citation statements)

References 95 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Additionally, experimentally working with SPF pigs has several advantages, above all the facilitation of the readout of the immune responses induced by our vaccines in almost an absence of background (very evident for the ELISPOT assays) and also the dissection of the mechanisms involved in immunoprotection, including immunodominance (9).…”

Section: Fig 4 Kinetics Of the Detection Of Ifn-␣ (A) And Tnf-␣ (B) Imentioning

confidence: 99%

“…Confirming the most-adverse previsions for 2014, ASF cases in wild boars have so far been reported in two countries from the European Union, Lithuania and Poland, where very recently an outbreak also affecting domestic pigs was declared. Despite the fact that little is known about the mechanisms involved in protection, seminal evidence has demonstrated the key role that humoral responses (5-7) and specific CD8 ϩ T cells (8,9) can play in protection. Future vaccine designs against African swine fever vi-rus (ASFV) should take lessons from these findings, garnered by using in vivo models of homologous protection with attenuated viruses first described in the 1960s (10)(11)(12).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

Lacasta

Ballester

Monteagudo

et al. 2014

J Virol

Self Cite

110

112

View full text Add to dashboard Cite

African swine fever is one of the most devastating pig diseases, against which there is no vaccine available. Recent work from our laboratory has demonstrated the protective potential of DNA vaccines encoding three African swine fever viral antigens (p54, p30, and the hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in the absence of detectable antibodies prior to virus challenge, and survival correlated with the presence of a large number of hemagglutinin-specific CD8 IMPORTANCEAfrican swine fever is a highly contagious disease of domestic and wild pigs that is endemic in many sub-Saharan countries, where it causes important economic losses and is currently in continuous expansion across Europe. Unfortunately, there is no treatment nor an available vaccine. Early attempts using attenuated vaccines demonstrated their potential to protect pigs against experimental infection. However, their use in the field remains controversial due to safety issues. Although inactive and subunit vaccines did not confer solid protection against experimental ASFV infection, our DNA vaccination results have generated new expectations, confirming the key role of T-cell responses in protection and the existence of multiple ASFV antigens with protective potential, more of which are currently being identified. Thus, the future might bring complex and safe formulations containing more than a single viral determinant to obtain broadly protective vaccines. We believe that obtaining the optimal vaccine formulation it is just a matter of time, investment, and willingness.

show abstract

Section: Fig 4 Kinetics Of the Detection Of Ifn-␣ (A) And Tnf-␣ (B) Imentioning

confidence: 99%

mentioning

confidence: 99%

Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

Lacasta

Ballester

Monteagudo

et al. 2014

J Virol

Self Cite

110

112

View full text Add to dashboard Cite

show abstract

“…The underlying mechanisms are far from being fully understood. Animals surviving infection with low-virulence strains are only protected against highly virulent related strains, and the cellular component of the immune response is fundamental for this protection (Takamatsu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Related strains of African swine fever virus with different virulence: genome comparison and analysis

Portugal

Coelho

Höper

et al. 2015

Journal of General Virology

Self Cite

110

View full text Add to dashboard Cite

“…Nevertheless, a humoral response is essential but not enough for protection against ASFV. Some studies clearly demonstrate that T-cells play an important role in ASF protection, even in the absence of specific antibodies (59). Therefore, we can conclude that, based on the experiments performed so far, a potential vaccine for ASF should activate both humoral and cellular responses, as well as satisfying safety requirements.…”

Section: Vaccinementioning

confidence: 79%

New insights into the role of ticks in African swine fever epidemiology

Sánchez-Vizcaı́no¹,

Mur²,

Bastos³

et al. 2015

Rev. Sci. Tech. OIE

View full text Add to dashboard Cite

SummaryAfrican swine fever (ASF), one of the most important diseases of swine, is present in many African countries, as well as in eastern Europe, Russia and Sardinia. It is caused by a complex virus, ASF virus (ASFV), for which neither vaccine nor treatment is available. ASFV affects swine of all breeds and ages, and also replicates in soft ticks of the genus Ornithodoros, facilitating ASFV persistence and reocurrence of disease. Depending on the involvement of these ticks, and the presence or not of sylvatic asymptomatic animals, several epidemiological cycles have been identified. The disease persists in East and southern African countries in a sylvatic cycle between O. porcinus (of the O. moubata species complex) and common warthogs. In some countries a domestic pig-tick cycle exists, whereas in other regions, notably West Africa, the role of soft ticks has not been demonstrated, and ASFV is transmitted between domestic pigs in the absence of tick vectors. Even in several East and Central African countries which have the sylvatic or domestic cycle, the majority of outbreaks are not associated with ticks or wild suids. In Europe, O. erraticus was detected and identified as a crucial vector for ASF maintenance in outdoor pig production on the Iberian Peninsula. However, in most parts of Europe, there is a lack of information about the distribution and role of Ornithodoros ticks in ASF persistence, particularly in eastern regions. This article reviews ASF epidemiology and its main characteristics, with a special focus on the distribution and role of soft ticks in ASF persistence in different settings. Information about tick detection, control measures and future directions for research is also included.

show abstract

Cellular immunity in ASFV responses

Cited by 138 publications

References 95 publications

Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

Related strains of African swine fever virus with different virulence: genome comparison and analysis

New insights into the role of ticks in African swine fever epidemiology

Contact Info

Product

Resources

About