Cellular Interactions with Self-assembled Biomaterials and Composites for Bone Tissue Engineering

Vines, Jeremy B.; Alexander, Grant C. B.; Deng, Lily; Hwang, Patrick T.J.; Patel, Dhruv; Jun, Ho−Wook

doi:10.1007/978-3-319-21813-7_5

Cited by 1 publication

(1 citation statement)

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“…Orthopedic implants often require an extra surgery because of presenting a lack of three of the most critical characteristics of living tissues: (1) the ability to self-repair, (2) the ability to maintain a blood supply and (3) the capacity to modify their particular structure and properties in response to the local environment 3 . Bone tissue engineering (BTE) arises to solve those problems, promoting the self-regeneration of those diseased or damaged bone tissues 4 . The cornerstone of BTE is the synergistic combination of osteogenic cells, biocompatible framework or scaffold, vascularization and specialized culture conditions with suitable biochemical and physical stimuli to ultimately promoting ingrowth and physiological restoration of bone 5 - 7 .…”

Section: Introductionmentioning

confidence: 99%

Novel antimicrobial phosphate-free glass–ceramic scaffolds for bone tissue regeneration

Suárez

Fernández-García

Fernández

et al. 2020

Sci Rep

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In this study a phosphate-free glass-ceramic porous scaffold was synthesized by a three-step methodology involving slurry preparation, induction of porosity by surfactant-assisted foaming following by freeze-drying and sintering. This inorganic scaffold was characterized by X-ray diffraction, scanning electron microscope (SEM), degradation and bioactivity. Thermal treatment at 750 °C showed two new crystalline phases, combeite and nepheline, into the glassy matrix responsible for its properties. The cell response of the scaffold was also evaluated for using as a bone graft substitute. A commercial Biphasic Calcium Phosphate, BCP, scaffold was assessed in parallel as reference material. Microstructures obtained by SEM showed the presence of macro, meso and microporosity. The glass-ceramic scaffold possesses an interconnected porosity around 31% with a crack-pore system that promote the protein adsorption and cell attachment. Glass-ceramic scaffold with high concentration of calcium ions shows an antimicrobial behavior against Escherichia coli after 24 h of contact. nepheline phase present in the glass-ceramic structure is responsible for its high mechanical properties being around 87 MPa. Glass-ceramic scaffold promotes greater protein adsorption and therefore the attachment, spreading and osteodifferentiation of Adipose Derived Stem Cells than BCP scaffold. A higher calcification was induced by glass-ceramic scaffold compared to reference BCP material.

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