Cellular internalization and release of polystyrene microplastics and nanoplastics

Liu, Ling; Xu, Kexin; Zhang, Bowen; Ye, Yiyuan; Zhang, Qiu; Jiang, Wei

doi:10.1016/j.scitotenv.2021.146523

Cited by 202 publications

(90 citation statements)

References 49 publications

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“…To probe favorable interactions between GFMs and lipids for explaining the GFM induced PS layer damage, large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs) composed of DOPC and DOPG lipids were prepared using the extruding method and gentle hydration procedure, respectively [ 62 ]. These two unsaturated lipid species, despite being different from real components of natural PS, were adopted for ease of preparing vesicles, and have been widely used for studies of membrane interactions with nanomaterials [ 63 ].…”

Section: Methodsmentioning

confidence: 99%

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Competitive and/or cooperative interactions of graphene-family materials and benzo[a]pyrene with pulmonary surfactant: a computational and experimental study

Yue

et al. 2021

Part Fibre Toxicol

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Background Airborne nanoparticles can be inhaled and deposit in human alveoli, where pulmonary surfactant (PS) molecules lining at the alveolar air–water interface act as the first barrier against inhaled nanoparticles entering the body. Although considerable efforts have been devoted to elucidate the mechanisms underlying nanoparticle-PS interactions, our understanding on this important issue is limited due to the high complexity of the atmosphere, in which nanoparticles are believed to experience transformations that remarkably change the nanoparticles’ surface properties and states. By contrast with bare nanoparticles that have been extensively studied, relatively little is known about the interactions between PS and inhaled nanoparticles which already adsorb contaminants. In this combined experimental and computational effort, we investigate the joint interactions between PS and graphene-family materials (GFMs) with coexisting benzo[a]pyrene (BaP). Results Depending on the BaP concentration, molecular agglomeration, and graphene oxidation, different nanocomposite structures are formed via BaPs adsorption on GFMs. Upon deposition of GFMs carrying BaPs at the pulmonary surfactant (PS) layer, competition and cooperation of interactions between different components determines the interfacial processes including BaP solubilization, GFM translocation and PS perturbation. Importantly, BaPs adsorbed on GFMs are solubilized to increase BaP’s bioavailability. By contrast with graphene adhering on the PS layer to release part of adsorbed BaPs, more BaPs are released from graphene oxide, which induces a hydrophilic pore in the PS layer and shows adverse effect on the PS biophysical function. Translocation of graphene across the PS layer is facilitated by BaP adsorption through segregating it from contact with PS, while translocation of graphene oxide is suppressed by BaP adsorption due to the increase of surface hydrophobicity. Graphene extracts PS molecules from the layer, and the resultant PS depletion declines with graphene oxidation and BaP adsorption. Conclusion GFMs showed high adsorption capacity towards BaPs to form nanocomposites. Upon deposition of GFMs carrying BaPs at the alveolar air–water interface covered by a thin PS layer, the interactions of GFM-PS, GFM-BaP and BaP-PS determined the interfacial processes of BaP solubilization, GFM translocation and PS perturbation.

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Section: Methodsmentioning

confidence: 99%

“…Lipid extraction by GFMs was monitored by QCM-D equipped with an Au-coated crystal sensor [ 62 , 65 ]. In brief, ultrapure water and Tris solution (10 mM Tris and 150 mM NaCl) were flowed through the sensor for 10 min, respectively, to obtain a stable baseline for experiments.…”

Section: Methodsmentioning

confidence: 99%

Competitive and/or cooperative interactions of graphene-family materials and benzo[a]pyrene with pulmonary surfactant: a computational and experimental study

Yue

et al. 2021

Part Fibre Toxicol

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“…Small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs) composed of DOPC and DOPG were prepared using the extruding method and gentle hydration procedure, respectively. [51] DOPC and DOPG stock solutions were obtained by dissolving lipids in a mixed solution of trichloromethane and methanol (2:1, V/V) and the nal concentration was 18 mg/mL and 2 mg/mL, respectively. 50 μL DOPC and 50 μL DOPG solutions were su ciently mixed in glass vials.…”

Section: Vesicle Preparationmentioning

confidence: 99%

“…Lipid extraction was monitored by QCM-D equipped with an Au-coated crystal sensor [51,53]. In brief, ultrapure water and Tris solution (10 mM Tris and 150 mM NaCl) were owed through the sensor for 10 min, respectively, to obtain a stable baseline for experiments.…”

Section: Qcm-d Experimentsmentioning

confidence: 99%

Joint Interactions of Graphene and Benzo[a]pyrene With Pulmonary Surfactant

Yue

et al. 2021

Preprint

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Background: Airborne nanoparticles can be inhaled and deposit in human alveoli, where pulmonary surfactant (PS) molecules line at the alveolar air-water interface to act as the first barrier against inhaled nanoparticles entering the body. Although considerable efforts have been made to elucidate the mechanisms underlying nanoparticle-PS interactions, our understanding on this important issue is limited due to the high complexity of the atmosphere, in which nanoparticles are believed to experience transformations that remarkably change the nanoparticles’ surface properties and states. By contrast with bare nanoparticles that have been extensively studied, relatively little is known about the interactions between PS and inhaled nanoparticles which already adsorb contaminants. In this combined experimental and computational effort, we investigate the joint interactions between PS and graphene with coexisting benzo[a]pyrene (BaP).Results: Depending on the BaP concentration and molecular agglomeration, different nanocomposite structures are formed via BaPs adsorption on graphene. Upon deposition of graphene carrying BaPs at the pulmonary surfactant (PS) layer, competition of interactions between different components determines the interfacial processes including BaP solubilization, graphene translocation and PS perturbation. Importantly, BaP adsorbed on graphene is solubilized to increase its bioavailability and inhibit the PS biophysical function. Translocation of graphene across the PS layer is facilitated by BaP adsorption through segregating it from contact with PS, while translocation of graphene oxide is suppressed due to increase of the surface hydrophobicity. Graphene extracts PS molecules from the layer, and the resultant PS depletion declines with graphene oxidation and BaP adsorption.Conclusion: Graphene showed high capacity of adsorbing BaPs to form nanocomposites, which were inhaled and deposit in alveoli, where competition of interactions between different components determined the interfacial processes of BaP solubilization, graphene translocation and PS perturbation.

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“…Literature regarding nanoplastics is growing exponentially, but due to the complexity of separating nanoplastics from environmental samples, 15,16 most of the studies conducted so far have been carried out on model nanoparticles, such as synthetic polystyrene nanobeads. 17,18,19,20 The toxicity of these synthetic polymeric nanoparticles has been extensively investigated on cells and marine organisms at different trophic levels, evidencing several biological dysfunctions. 21,22,23,24,25,26,10 In addition, nanoplastics could be even more dangerous than their micrometric counterpart because, due to nanometric size, they are thought to be able to penetrate tissues and biological barriers.…”

Section: Introductionmentioning

confidence: 99%

A Different Protein Corona Cloaks “True-to-Life” Nanoplastics with Respect to Synthetic Polystyrene Nanobeads

Ducoli¹,

Federici

Nicsanu³

et al. 2021

Preprint

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Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions of daily-life plastic items, we produced “true-to-life” nanoplastics (T2LNPs), that promises to give a true insight into the interaction with biological systems. T2LNPs have been fully characterized by Fourier transform Infrared spectroscopy and by Atomic Force Microscopy. They result in populations of spheroidal nanoparticles with a broad multimodal size distribution. The mandatory need for a representative sample to evaluate the potential effects of nanoparticles on human health and the environment is demonstrated by the different protein corona identified on T2LNPs and synthetic polystyrene nanobeads upon incubation with human plasma.

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Cellular internalization and release of polystyrene microplastics and nanoplastics

Cited by 202 publications

References 49 publications

Competitive and/or cooperative interactions of graphene-family materials and benzo[a]pyrene with pulmonary surfactant: a computational and experimental study

Competitive and/or cooperative interactions of graphene-family materials and benzo[a]pyrene with pulmonary surfactant: a computational and experimental study

Joint Interactions of Graphene and Benzo[a]pyrene With Pulmonary Surfactant

A Different Protein Corona Cloaks “True-to-Life” Nanoplastics with Respect to Synthetic Polystyrene Nanobeads

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