Cellular Kinetics of 19S and 7S Hemolysin Production in Mice under the Influence of Bacterial Endotoxins

Finger, H; Beneke, G; Fresenius, H.

doi:10.1159/000162243

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The other is the ability to confer some degree of resistance to low challenge with peritoneal cells from mice immunized with heatkilled bacteria. Finger et al (11) have recently demonstrated that heat-killed gram-negative vaccines elicit splenic hyperplasia. Similarly, Cluff (4) reported that endotoxin-containing vaccines nonspecifically increased the enzymatic content of macrophagic cells and consequently enhanced the resistance of animals to experimental infection by bacterial, fungal, or virus parasites.…”

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confidence: 99%

Cell-Mediated Resistance Induced with Immunogenic Preparations of Salmonella typhimurium

Venneman

Berry

1971

Infect Immun

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Peritoneal cells obtained from mice immunized 15 or 30 days previously with (i) 0.1 LDw of attenuated Salmonella typhimurium (RIA), (ii) 20 jug (dry weight) of heat-killed Salmonella (SR-11), (iii) 20 Ag (dry weight) of immunogenic ribosomal subfractions, or (iv) 20 Ag of ribonucleic acid (RNA) subfractions were passively transferred to normal unimmunized mice. The ability of the recipient animals to inhibit or retard the multiplication of virulent challenge S. typhimurium 5 days postinfection was determined by pathogen counts on the carcasses. Peritoneal cells from donors immunized with the RIA, ribosomal, or RNA preparations (i) conferred maximal resistance to challenge 10 to 15 days after cell transfer and demonstrable resistance throughout the 45-day assay period, (ii) conferred resistance to infection when 105, 103, or 102 peritoneal cells were injected subcutaneously but not with fewer than 105 cells administered intraperitoneally, and (iii) rendered recipients capable of acting as donors of peritoneal cells that conferred demonstrable resistance on normal recipients. Recipients of peritoneal cells obtained from donors immunized with heat-killed bacteria were unable to (i) significantly inhibit bacterial proliferation at 10 days post-transfer, (ii) resist infection by a challenge inoculum greater than 50 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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confidence: 99%

Cell-Mediated Resistance Induced with Immunogenic Preparations of Salmonella typhimurium

Venneman

Berry

1971

Infect Immun

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“…The method was based on that of Finger and his co-workers (Finger, Beneke & Fresenius, 1970;Finger, Emmerling & Bruss, 1970). Groups of five mice (Theiler's Original strain) were used; the adjuvant to be tested (0.25 ml) was given intraperitoneally, followed by the antigen, 0.25 ml of 1/2.5 dilution of human group A whole blood in buffered saline.…”

Section: Introductionmentioning

confidence: 99%

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Ackers

Dolby

1972

Journal of General Microbiology

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S U M M A R YThe ability to protect mice against intracerebral infection and to elicit, after one dose, complement-mediated bactericidal antibody capable of killing a mousevirulent phase I strain in vitro, was tested in seven cultures of Bordetellapertussis: three typical phase I strains; two strains grown in the presence of 0.5 mg nicotinic acid/ml ; one phase IV strain ; and the atypical strain 134. The typical strains showed both activities; the nicotinic acid-grown and phase IV strains elicited high antibody titres, but did not confer protection, whereas strain 134 protected, but did not elicit bactericidal antibody.Pyrogenic material extracted from dried organisms by hot phenol and water (lipopolysaccharide) was not antigenic in mice; however, with all strains, except 134, multiple doses of this material coupled to stromata or Escherichiu coli conjugated protein elicited bactericidal and precipitating antisera in mice, although the mice were not protected.Strain 134 contained approximately one quarter of the normal amount of endotoxin, as assayed in actinomycin D-sensitized mice, but was as effective as normal strains as an adjuvant for haemolysin production.

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“…Both PO and ET cause an increase in the blood leukocyte counts, this being associated with a significant and long-term elevation of the spleen weights [Morse, 1965;F inger et al, 1970a]. Thereby, the effectiveness of PO was found to be much stronger, as compared to that of ET [Finger et al, 1970a].…”

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confidence: 99%

Influence of Pertussis Vaccine and Bacterial Endotoxin on Blood Leukocyte Counts and Numbers of Pre-Existing Antibody-Forming Cells in Aged Mice

Finger¹,

H²

1972

Gerontology

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The present studies were carried out in order to explore, whether or not senile mice react in the same manner to a single injection of either 50 μg Serratia marcescens endotoxin (ET) or 3 × 10⁹ killed Bordetella pertussis organisms (PO) as young adult mice. This was not the case. The following essential differences were observed. (1) Aged mice produce PO-mediated blood leukocytosis to a distinctly reduced extent, as compared to young adult mice. (2) The injection of either ET or PO into adult mice led to a short-lived, but significant increase in the numbers of cells producing 19S hemolysins to sheep erythrocytes, whilst the response regarding those cells synthesizing 19S antibodies to rabbit erythrocytes was very poor. Thereby, the reactivity of young adult mice was found to be very homogenous. In contrast to untreated young adult mice, the spleens of untreated senile animals contained on the average larger numbers of plaque-forming cells, the values differing strongly from spleen to spleen. Following treatment with either ET or PO the senile mice showed extreme differences regarding their capacity to react with an increase in hemolysin-forming spleen cells, thereby, reaching the maximum response in each case later than young adult mice. (3) There was no close positive relationship between the existence of cells producing 19S hemolysins to sheep erythrocytes and the occurrence of those synthesizing corresponding antibodies to rabbit erythrocytes. (4) Aged mice showed about a 10-fold increase to the lethal action of ET, as compared to young adult mice.

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Cellular Kinetics of 19S and 7S Hemolysin Production in Mice under the Influence of Bacterial Endotoxins

Cited by 5 publications

References 15 publications

Cell-Mediated Resistance Induced with Immunogenic Preparations of Salmonella typhimurium

Cell-Mediated Resistance Induced with Immunogenic Preparations of Salmonella typhimurium

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Influence of Pertussis Vaccine and Bacterial Endotoxin on Blood Leukocyte Counts and Numbers of Pre-Existing Antibody-Forming Cells in Aged Mice

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