Cellular Mechanisms of Organic Anion Transport in Crustacean Renal Tissue

Holliday, Charles W.; Miller, David S.

doi:10.1093/icb/24.1.275

Cited by 25 publications

(19 citation statements)

References 9 publications

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“…However, in some instances we could collect enough data from females to draw limited conclusions about male-female differences. In Homarus, there were no differences in rates of organic anion transport (33), but bladders from females exhibited 50% greater flux ratios for organic cations than males (Table 7). In Menippe, organic anion flux ratios in males were twice those in females.…”

Section: Crab Urinary Bladdermentioning

confidence: 96%

“…Our findings, along with some from other laboratories, are summarized in Table 6, which lists the many functional analogies between crustacean urinary bladder and vertebrate proximal tubule. Among these are the ability of the bladder to reabsorb nutrients and to transport organic anions and cations (29)(30)(31)(32)(33), processes localized solely to the proximal segment in ver- FIGURE 6. Electron micrograph of urinary bladder tissue from Cancer magister.…”

Section: Crab Urinary Bladdermentioning

confidence: 99%

“…Reabsorption of sugars and amino acids Reabsorption of NaCl and water Secretion of organic cations Secretion of organic anions (some crustacean species, others reabsorb; see text) Classified as electrically "leaky" epithelia aData supporting these conclusions may be found in the following reviews: for proximal tubule (8); for crustacean bladder (33,41). tebrate kidney.…”

Section: Crab Urinary Bladdermentioning

confidence: 99%

“…In decapod crustacea, the bladder is the terminal element of the antennal gland, which functions as a kidney analog. The gland contains an ultrafiltration site, the coelomosac; an extensive labyrinth, capable of solute transport; in some species, a true tubular segment; and the extensive urinary bladder (33). These are arranged in series.…”

Section: Crab Urinary Bladdermentioning

confidence: 99%

“…In C. irroratus only, an unidentified metabolite was excreted through the nonrenal pathway. (29,(31)(32)(33). Flux ratios greater than unity indicate net solute secretion; those less than unity indicate net reabsorption.…”

Section: Crab Urinary Bladdermentioning

confidence: 99%

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Aquatic models for the study of renal transport function and pollutant toxicity.

Miller¹

1987

Environ Health Perspect

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Studies of renal cell transport mechanisms and their impairment by xenobiotics are often limited by technical difficulties related to renal tubule complexity. Problems include the juxtaposition of multiple tubule segments with different transport functions and severely limited access to the tubular lumen. Some limitations can be overcome by the careful selection of an appropriate aquatic experimental system. Two aquatic models for the vertebrate proximal segment are discussed here. The first is the kidney from certain marine flounder, which offers the following advantages: long-term viability, little tissue of nonproximal origin, and easy tubule isolation. Data are presented to demonstrate how studies with flounder kidney can be used to elucidate cellular mechanisms whereby different classes of toxic pollutants may interact. Results from these experiments indicate that the excretion of certain anionic xenobiotics can be delayed (1) by other anionic xenobiotics that compete for secretory transport sites and (2) by compounds that disrupt cellular ion gradients and energy metabolism needed to drive transport.The second system is the crustacean urinary bladder, a simple, flatsheet epithelium. Bladder morphology and transport physiology closely resemble those of vertebrate proximal segment. Electron micrographs show a brush border membrane at the luminal surface, numerous mitochondria, and an infolded serosal membrane, while in vivo and in vitro transport studies show reabsorption of NaCl, nutrients and water and secretion of organic cations; organic anions are secreted in bladders from some species and reabsorbed in others. Moreover, since bladders can be mounted as flat sheets in flux chambers, studies with this tissue avoid the problems of complex renal tubule geometry and tissue heterogeneity that limit transport studies in proximal tubule.

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Section: Crab Urinary Bladdermentioning

confidence: 96%

Section: Crab Urinary Bladdermentioning

confidence: 99%

Section: Crab Urinary Bladdermentioning

confidence: 99%

Section: Crab Urinary Bladdermentioning

confidence: 99%

Section: Crab Urinary Bladdermentioning

confidence: 99%

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Aquatic models for the study of renal transport function and pollutant toxicity.

Miller¹

1987

Environ Health Perspect

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Ultracytochemical location of Na+/K+-atpase activity and effect of high salinity acclimation in gill and renal epithelia of the freshwater shrimpMacrobrachium olfersii (Crustacea, Decapoda)

McNamara

Torres

1999

J. Exp. Zool.

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Accumulation sites of lead phosphate reaction product consequent to Na+/K+‐ATPase activity in gill and renal epithelia of the freshwater shrimp Macrobrachium olfersii were located ultracytochemically by para‐nitrophenyl‐phosphate hydrolysis and lead precipitation, and quantified per unit membrane area and cytoplasmic volume. In shrimps in freshwater (<0.5‰ S, 20 mOsm/kg H2O, 0.7 mEq Na+/liter), numerous sites of electron‐dense, Na+/K+‐ATPase reaction product accumulation were demonstrated in the membrane invaginations of the mitochondria‐rich, intralamellar septal cells (12.5 ± 1.7 sites/μm2 membrane, 179 ± 22 sites/μm3 cytoplasm, mean± SEM, N ≤ 7) and in the basal region of the medial renal tubules (19.8 ± 1.8 sites/μm2 membrane, 437 ± 53 sites/μm3 cytoplasm), but not in the pillar cells whose apical flanges form the primary interface with the external medium. A putative, ouabain‐insensitive Na+‐ or H+‐ATPase was found in the apical microvilli of the medial renal tubules (17.4 ± 1.7 sites/μm2 membrane, 629 ± 101 sites/μm3 cytoplasm). This restricted location of Na+/K+‐ATPase activity within the gill epithelium suggests that during uptake, Na+ moves across the apical pillar cell membrane, passes through specialized, basolateral coupling junctions into the septal cell cytoplasm and is pumped into the hemolymph via the Na+/K+‐ATPase in the invagination membranes. In shrimps acclimated to a high‐salinity medium (21‰ S, 630 mOsm/kg H2O, 280 mEq Na+/liter) for 2 and 5 days, the mean number of sites of para‐nitrophenylphosphatase activity/μm2 membrane and /μm3 cytoplasm for both epithelia increases markedly by 83 and 163%, respectively. However, after 10 days acclimation, the number of sites declines dramatically, attaining values far below those for shrimps in freshwater. These acclimation‐induced alterations in numerical density/μm3 cytoplasm cannot be accounted for by corresponding changes in membrane surface density (μm2 membrane/μm3 cytoplasm) and reflect a real alteration in the number of Na+/K+‐ATPase reaction product sites/unit membrane area. These data suggest that neither the gill nor the renal Na+/K+‐ATPase systems function at maximal activity in shrimps in freshwater, possibly due to the low Na+ concentration, and are initially stimulated by the increase in external ionic concentration. However, these powerful Na+ transport systems respond to salt loading by a notable reduction in the number of hydrolysis sites, possibly through the incorporation of the Na+/K+‐ATPase into isolated membrane vesicles in the basal invaginations of the medial renal tubules, together with ultrastructural alterations like the spatial isolation of the mitochondria by multiple membrane stacks in the intralamellar septal cells. J. Exp. Zool. 284:617–628, 1999. © 1999 Wiley‐Liss, Inc.

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Renal Excretion and Tubular Transport of Organic Anions and Cations

Roch-Ramel

Besseghir

Murer

1992

Comprehensive Physiology

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The sections in this article are: Organic Anions Introduction Renal Excretion and Tubular Transport of Organic Anions Mechanisms Involved in Tubular Transport of Organic Anions Conclusions on the Renal Transport of Organic Anions Organic Cations Introduction Renal Excretion and Tubular Transport of Organic Cations Mechanisms Involved in Tubular Transport of Organic Cations General Conclusions

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Cellular Mechanisms of Organic Anion Transport in Crustacean Renal Tissue

Cited by 25 publications

References 9 publications

Aquatic models for the study of renal transport function and pollutant toxicity.

Aquatic models for the study of renal transport function and pollutant toxicity.

Ultracytochemical location of Na+/K+-atpase activity and effect of high salinity acclimation in gill and renal epithelia of the freshwater shrimpMacrobrachium olfersii (Crustacea, Decapoda)

Renal Excretion and Tubular Transport of Organic Anions and Cations

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