Cellular membrane potentials induced by alternating fields

Grosse, Constantino; Schwan, Herman P.

doi:10.1016/s0006-3495(92)81740-x

Cited by 229 publications

(142 citation statements)

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“…The value of induced transmembrane potential sustainable for living cell electroporation was determined to be 1 V (Zimmermann 1982;Tsong 1991). Later the value of the induced transmembrane potential that triggers electroporation was determined to be in the range of 200-500 mV (Marszalek et al 1990;Grosse and Schwan 1992;Teissié et al 1993). These values obtained by fluorescence imaging and calculations were further confirmed by direct measurement at the single-cell level using patch clamp technique (Ryttsen et al 2000).…”

Section: From Single-cell To Tissuementioning

confidence: 87%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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In this chapter, basics and mechanisms of electroporation are presented. Most important electric pulse parameters for electroporation efficiency for different applications that involve introduction of small molecules and macromolecules into the cell or cell membrane electrofusion are described. In all these applications, cell viability has to be preserved. However, in some biotechnological applications, such as liquid food sterilization or water treatment, electroporation is used as a method for efficient cell killing. For all the applications mentioned above, besides electric pulse parameters, other factors, such as electroporation medium composition and osmotic pressure, play significant roles in electroporation effectiveness. For controlled use of the method in all applications, the basic mechanisms of electroporation need to be known. The phenomenon was studied from the single-cell level and dense cell suspension that represents a simplified homogenous tissue model, to complex biological tissues. In the latter, different cell types and electric conductivity that change during the course of electric pulse application can significantly affect the effectiveness of the treatment. For such a complex situation, the design and use of suitable electrodes and theoretical modeling of electric field distribution within the tissue are essential. Electroporation as a universal method applicable to different cell types is used for different purposes. In medicine it is used for electrochemotherapy and genetherapy. In biotechnology it is used for water and liquid food sterilization and for transfection of bacteria, yeast, plant protoplast, and intact plant tissue. Understanding the phenomenon of electroporation, its mechanisms and optimization of all the parameters that affect electroporation is a prerequisite for successful treatment. In addition to the parameters mentioned above, different biological characteristics of treated cell affect the outcome of the treatment. Electroporation, gene electrotransfer and electrofusion are affected by cell membrane fluidity, cytoskeleton, and the presence of the cell wall in bacteria yeast and plant cells. Thus, electroporation parameters need to be specifically optimized for different cell types.

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Section: From Single-cell To Tissuementioning

confidence: 87%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

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“…In general, (5a) is applicable to exposures to all rectangular electric pulses longer than 1 µs, as well as all sine (AC) electric fields with frequencies up to 1 MHz. To determine ΔΨ m induced by shorter pulses or higher frequencies, the permittivities of the electrolytes surrounding the membrane also have to be taken into account, leading to further generalization of (5) to a second-order model [35]- [38].…”

Section: Evaluation Of Induced Voltagementioning

confidence: 99%

Cell membrane electroporation- Part 1: The phenomenon

Kotnik

Kramar

Pucihar

et al. 2012

IEEE Electr. Insul. Mag.

413

212

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“…Schwan developed the first theoretical equation for the induced potential in a spherical cell in suspension exposed to external EF by analytical solution of the Laplace equation, where the cell is approximated by a spherical shell representing the cell membrane [48]. The model by Shawn treats the cell as a non-conducting membrane subjected to a constant and alternating external EF [49]. Kotnik et al [50] extended Schwan's theory by taking into account the conductivity using constant, oscillating and pulsed EF.…”

Section: Introductionmentioning

confidence: 99%

Modulation of cell function by electric field: a high-resolution analysis

Taghian

Narmoneva

Kogan

2015

J. R. Soc. Interface.

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Regulation of cell function by a non-thermal, physiological-level electromagnetic field has potential for vascular tissue healing therapies and advancing hybrid bioelectronic technology. We have recently demonstrated that a physiological electric field (EF) applied wirelessly can regulate intracellular signalling and cell function in a frequency-dependent manner. However, the mechanism for such regulation is not well understood. Here, we present a systematic numerical study of a cell-field interaction following cell exposure to the external EF. We use a realistic experimental environment that also recapitulates the absence of a direct electric contact between the field-sourcing electrodes and the cells or the culture medium. We identify characteristic regimes and present their classification with respect to frequency, location, and the electrical properties of the model components. The results show a striking difference in the frequency dependence of EF penetration and cell response between cells suspended in an electrolyte and cells attached to a substrate. The EF structure in the cell is strongly inhomogeneous and is sensitive to the physical properties of the cell and its environment. These findings provide insight into the mechanisms for frequency-dependent cell responses to EF that regulate cell function, which may have important implications for EF-based therapies and biotechnology development.

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Cellular membrane potentials induced by alternating fields

Cited by 229 publications

References 0 publications

Electroporation in Biological Cell and Tissue: An Overview

Electroporation in Biological Cell and Tissue: An Overview

Cell membrane electroporation- Part 1: The phenomenon

Modulation of cell function by electric field: a high-resolution analysis

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