Cellular Networks of Human Thymic Medullary Stromas Coordinated by p53-Related Transcription Factors

Ichimiya, Shingo; Kojima, Takashi

doi:10.1369/jhc.6a7028.2006

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…Of special interest is that the typical mTEC‐associated cytokeratins K5 and K14 were found in both cells and exosomes, while the typical cortical thymic epithelial cell (cTEC) associated cytokeratin K8 was only identified in cells. Besides the cytokeratins, other typical TEC proteins were found in the cells, for example, involucrin, a marker for late‐stage mTEC differentiation and CLAUDIN‐1 16 . Amidst exosome‐specific proteins ( n =270), not found in the cells, classical exosomal markers such as TSG101, CD82, CD63, MFG‐E8 and FLOTILIN‐1 were present.…”

Section: Resultsmentioning

confidence: 99%

“…Besides the cytokeratins, other typical TEC proteins were found in the cells, for example, involucrin, a marker for late-stage mTEC differentiation and CLAUDIN-1. 16 Amidst exosome-specific proteins (n = 270), not found in the cells, classical exosomal markers such as The exosomes stained positive for TSG101 and HLA-DR, weakly positive for CD9 and CD81 and negative for CD45. The exosomes were close to negative for CD63.…”

Section: The Proteomic Profiles Of Cultured Cells and Exosomes Includmentioning

confidence: 99%

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Human thymic epithelial primary cells produce exosomes carrying tissue‐restricted antigens

et al. 2015

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Exosomes are nano-sized vesicles released by cells into the extracellular space and have been shown to be present in thymic tissue both in mice and in humans. The source of thymic exosomes is however still an enigma and hence it is not known whether thymic epithelial cells (TECs) are able to produce exosomes. In this work, we have cultured human TECs and isolated exosomes. These exosomes carry tissue-restricted antigens (TRAs), for example, myelin basic protein and desmoglein 3. The presence of TRAs indicates a possible role for thymic epithelium-derived exosomes in the selection process of thymocytes. The key contribution of these exosomes could be to disseminate self-antigens from the thymic epithelia, thus making them more accessible to the pool of maturing thymocytes. This would increase the coverage of TRAs within the thymus, and facilitate the process of positive and negative selection.

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Section: Resultsmentioning

confidence: 99%

Section: The Proteomic Profiles Of Cultured Cells and Exosomes Includmentioning

confidence: 99%

Human thymic epithelial primary cells produce exosomes carrying tissue‐restricted antigens

et al. 2015

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“…RT‐PCR was conducted to detect certain transcripts, as previously reported 18. Primer pairs were selected using Primer3 software on mRNA sequences based on the NCBI database.…”

Section: Methodsmentioning

confidence: 99%

Tonsillar crypt epithelium of palmoplantar pustulosis secretes interleukin‐6 to support B‐cell development via p63/p73 transcription factors

Koshiba

Ichimiya

Nagashima

et al. 2007

The Journal of Pathology

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Palmoplantar pustulosis (PPP) is an autoimmune disease characterized by psoriasis-like erythematous lesions on palms and/or soles due to an abnormal humoral immune response. Tonsillectomy is effectively employed for the treatment of PPP; however, how tonsils are involved in the aetiology of PPP remains unclear. Here we analysed surgically resected palatine tonsils from 36 cases of PPP as well as usual recurrent tonsillitis (RT) as a control. Histological examination revealed that a unique lesion, with lymphoid follicles surrounded by reticular crypt epithelial cells, was more frequently observed in tonsils of patients with PPP than in those with RT (p < 0.0001; PPP vs RT). Interestingly, crypt epithelial cells in primary cultures derived from PPP tonsils showed marked production of interleukin-6 (IL-6). Moreover, these epithelial cells from PPP tonsils expressed p53-related transcription factors in their nuclei that were found to contribute to the up-regulation of IL-6 gene expression. These findings suggest that, at least in part, the specialized lymphoepithelial symbiosis of PPP tonsils, under the control of p53-related factors, may be relevant to the generation of the impaired micro-environment underlying the aberrant production of autoantibodies.

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“…The main function of this interaction is the transferring of small molecules. Their expression is under the control of p53 family members (Ichimiya and Kojima 2006). The lack of p53 conducts to an histological abnormalities of thymus, due to the deep link to TJ (Celli et al 1999).…”

Section: Cell Interactionmentioning

confidence: 99%

Thymus and aging: morphological, radiological, and functional overview

Rezzani

Nardo

Favero

et al. 2013

AGE

159

112

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Aging is a continuous process that induces many alterations in the cytoarchitecture of different organs and systems both in humans and animals. Moreover, it is associated with increased susceptibility to infectious, autoimmune, and neoplastic processes. The thymus is a primary lymphoid organ responsible for the production of immunocompetent T cells and, with aging, it atrophies and declines in functions. Universality of thymic involution in all species possessing thymus, including human, indicates it as a long-standing evolutionary event. Although it is accepted that many factors contribute to age-associated thymic involution, little is known about the mechanisms involved in the process. The exact time point of the initiation is not well defined. To address the issue, we report the exact age of thymus throughout the review so that readers can have a nicely pictured synoptic view of the process. Focusing our attention on the different stages of the development of the thymus gland (natal, postnatal, adult, and old), we describe chronologically the morphological changes of the gland. We report that the thymic morphology and cell types are evolutionarily preserved in several vertebrate species. This finding is important in understanding the similar problems caused by senescence and other diseases. Another point that we considered very important is to indicate the assessment of the thymus through radiological images to highlight its variability in shape, size, and anatomical conformation.

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Cellular Networks of Human Thymic Medullary Stromas Coordinated by p53-Related Transcription Factors

Cited by 9 publications

References 52 publications

Human thymic epithelial primary cells produce exosomes carrying tissue‐restricted antigens

Human thymic epithelial primary cells produce exosomes carrying tissue‐restricted antigens

Tonsillar crypt epithelium of palmoplantar pustulosis secretes interleukin‐6 to support B‐cell development via p63/p73 transcription factors

Thymus and aging: morphological, radiological, and functional overview

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