Cellular Oxidative Stress

Dossena, Silvia; Marino, Angela

doi:10.3390/antiox10030399

Cited by 22 publications

(7 citation statements)

References 28 publications

(27 reference statements)

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“…Aging is a dynamic chronological process characterized by the gradual accumulation of damage to cells, progressive functional decline, and increased susceptibility to disease [ 1 ]. A causal hypothesis that gained considerable interest in recent years postulates that pathophysiological changes during aging are due to progressive oxidative damage to cellular macromolecules [ 2 , 3 , 4 , 5 , 6 ]. In physiological conditions, the production of reactive oxygen and nitrogen species generated during cellular metabolism in biological systems is balanced by the ability of the latter to defend through their sophisticated antioxidant machinery.…”

Section: Introductionmentioning

confidence: 99%

Açaì (Euterpe oleracea) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress

Spinelli

Straface

Gambardella

et al. 2022

Cells

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Aging is a process characterised by a general decline in physiological functions. The high bioavailability of reactive oxygen species (ROS) plays an important role in the aging rate. Due to the close relationship between aging and oxidative stress (OS), functional foods rich in flavonoids are excellent candidates to counteract age-related changes. This study aimed to verify the protective role of Açaì extract in a d-Galactose (d-Gal)-induced model of aging in human erythrocytes. Markers of OS, including ROS production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups, as well as the anion exchange capability through Band 3 protein (B3p) and glycated haemoglobin (A1c) have been analysed in erythrocytes treated with d-Gal for 24 h, with or without pre-incubation for 1 h with 0.5–10 µg/mL Açaì extract. Our results show that the extract avoided the formation of acanthocytes and leptocytes observed after exposure to 50 and 100 mM d-Gal, respectively, prevented d-Gal-induced OS damage, and restored alterations in the distribution of B3p and CD47 proteins. Interestingly, d-Gal exposure was associated with an acceleration of the rate constant of SO42− uptake through B3p, as well as A1c formation. Both alterations have been attenuated by pre-treatment with the Açaì extract. These findings contribute to clarify the aging mechanisms in human erythrocytes and propose functional foods rich in flavonoids as natural antioxidants for the treatment and prevention of OS-related disease conditions.

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Section: Introductionmentioning

confidence: 99%

Açaì (Euterpe oleracea) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress

Spinelli

Straface

Gambardella

et al. 2022

Cells

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“…The aging process is characterized by a gradual accumulation of damage to cells, progressive functional decline, and increased susceptibility to chronic disease [ 1 ]. A causal hypothesis that has gained considerable interest in recent years postulates that pathophysiological changes during aging are due to progressive oxidative damage to cellular macromolecules [ 2 , 3 , 4 , 5 , 6 ]. During cellular metabolism, the production of reactive oxygen and/or nitrogen species (ROS and/or RNS) generated in biological systems is balanced by the ability of the latter to protect themselves through their endogenous antioxidant machinery [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Aging Injury Impairs Structural Properties and Cell Signaling in Human Red Blood Cells; Açaì Berry Is a Keystone

et al. 2023

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Red blood cell (RBC) deformability is the ability of cells to modulate their shape to ensure transit through narrow capillaries of the microcirculation. A loss of deformability can occur in several pathological conditions, during natural RBC aging through an increase in membrane protein phosphorylation, and/or through the structural rearrangements of cytoskeletal proteins due to oxidative conditions, with a key role played by band 3. Due to the close relationship between aging and oxidative stress, flavonoid-rich foods are good candidates to counteract age-related alterations. This study aims to verify the beneficial role of Açaì extract in a d-Galactose (d-Gal)-induced model of aging in human RBCs. To this end, band 3 phosphorylation and structural rearrangements in membrane cytoskeleton-associated proteins, namely spectrin, ankyrin, and/or protein 4.1, are analyzed in RBCs treated with 100 mM d-Gal for 24 h, with or without pre-incubation with 10 μg/mL Açaì extract for 1 h. Furthermore, RBC deformability is also measured. Tyrosine phosphorylation of band 3, membrane cytoskeleton-associated proteins, and RBC deformability (elongation index) are analyzed using western blotting analysis, FACScan flow cytometry, and ektacytometry, respectively. The present data show that: (i) Açaì berry extract restores the increase in band 3 tyrosine phosphorylation and Syk kinase levels after exposure to 100 mM d-Gal treatment; and (ii) Açaì berry extract partially restores alterations in the distribution of spectrin, ankyrin, and protein 4.1. Interestingly, the significant decrease in membrane RBC deformability associated with d-Gal treatment is alleviated by pre-treatment with Açaì extract. These findings further contribute to clarify mechanisms of natural aging in human RBCs, and propose flavonoid substances as potential natural antioxidants for the treatment and/or prevention of oxidative-stress-related disease risk.

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“…D-galactose (D-gal) is a reducing sugar that reacts readily with amino acid free amines in proteins and peptides in vitro and in vivo to form AGEs [ 7 ]. D-gal induces oxidative stress, and the accumulation of ROS under oxidative stress conditions leads to lipid peroxidation and glucose oxidation reactions, which also produce AGEs [ 8 , 9 ]. Increased AGEs hasten the aging process.…”

Section: Introductionmentioning

confidence: 99%

Propolis Ethanolic Extract Attenuates D-gal-induced C2C12 Cell Injury by Modulating Nrf2/HO-1 and p38/p53 Signaling Pathways

Tian

Zhao

Guo

et al. 2023

IJMS

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Previous study has shown that propolis ethanolic extract (PEE) has a protective effect on aging skeletal muscle atrophy. However, the exact molecular mechanism remains unclear. This study aimed to investigate the effect of PEE on D-galactose (D-gal)-induced damage in mouse C2C12 cells. The results revealed that PEE increased the viability of senescent C2C12 cells, decreased the number of senescence-associated β-galactosidase (SA-β-Gal)-positive cells and promoted the differentiation of C2C12 cells. PEE resisted oxidative stress caused by D-gal by activating the Nrf2/HO-1 signaling pathway and maintained the differentiation ability of C2C12 cells. PEE inhibited apoptosis by suppressing p38 phosphorylation and reducing p53 expression. In summary, our findings reveal the molecular mechanism by which PEE protects D-gal-induced C2C12 cells, providing a theoretical basis for the development of PEE for the alleviation of muscle atrophy.

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Cellular Oxidative Stress

Abstract: Oxidative stress on a cellular level affects the function of tissues and organs and eventually of the whole body [...]

Cited by 22 publications

References 28 publications

Açaì (Euterpe oleracea) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress

Açaì (Euterpe oleracea) Extract Protects Human Erythrocytes from Age-Related Oxidative Stress

Aging Injury Impairs Structural Properties and Cell Signaling in Human Red Blood Cells; Açaì Berry Is a Keystone

Propolis Ethanolic Extract Attenuates D-gal-induced C2C12 Cell Injury by Modulating Nrf2/HO-1 and p38/p53 Signaling Pathways

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