Cellular Prion Protein and Sexual Dimorphic Areas in Rodents. Correlates with Alzheimer Disease

Cuadrado‐Tejedor, Mar; Irujo, A.; Paternain, B.; Madrid, María Jesús; Velayos, J. L.

doi:10.4236/nm.2011.24051

Cited by 4 publications

(1 citation statement)

References 48 publications

(51 reference statements)

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“…[19], проаналізувавши ділянку кори та гіпокампа головного мозку методом вестерн-блот аналізу. В обох ділянках кількість PrP C збільшувалася в дорослих щурів (38 тижнів) порівняно з молодими (6 тижнів), а в старих тварин (56 тижнів) -зменшувала-ся.…”

Section: результати та обговоренняunclassified

Physiological prion and activity of plasma membrane Na(+),K(+)- and Ca(2+)-ATPase in the medulla oblongata of rats of different ages

Kushkevych¹,

Влизло²,

Martyn³

2013

Ukr.Biochem.J.

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Section: результати та обговоренняunclassified

Physiological prion and activity of plasma membrane Na(+),K(+)- and Ca(2+)-ATPase in the medulla oblongata of rats of different ages

Kushkevych¹,

Влизло²,

Martyn³

2013

Ukr.Biochem.J.

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Application of capillary immunoelectrophoresis revealed an age‐ and gender‐dependent regulated expression of PrPC in liver

et al. 2015

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The cellular prion protein (PrPC) is a glycoprotein, anchored to the plasma membrane and abundantly expressed in the central nervous system. The expression of PrPC in the peripheral tissues is low and only little information is available on its functions in the nonneuronal tissues. The antioxidant function of PrPC during the activation of hepatic stellate cells has already been reported. Therefore, the aim of the study was to expand our knowledge on the functions of PrPC by detailed characterization of its expressional profile in the liver. In a combined strategy by using capillary immunoelectrophoresis and standard techniques, we have shown a sexually dimorphic expression of PrPC in mice and human liver tissues. Further, we showed a significant age-dependent upregulation of PrPC expression in the liver of 14- and 9-month-old mice as compared to 3 months of age. Therefore, this study may provide new insights into the gender-specific role of PrPC in the liver, which may further be linked to its protective role against oxidative stress during aging. In addition, the current study also shows an application of immunoelectrophoresis with a low coefficient of variation to analyze the miniscule amount of PrPC in the mouse liver tissue.

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Qualitative analysis of cellular prion in the cerebellum of the wistar line rats of different age groups

Kushkevych¹,

Влизло²

2014

Bìol. Tvarin

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Prion infections (transmissible sponhioform encephalopathies (TSEs)) cause brain damage of humans and animals with lethal outcome. These diseases can be hereditary and sporadic. Neuronal degenerative changes, vacuolization of neurons, proliferation of astrocytes in the brain cortex, stem and cerebellum of the brain after death of TSE patients have been shown by histological research study. Cellular prion (PrP C ) is needed for replication of pathological PrP Sc which can cause infection development depending on the level of PrP C production in the different tissues. PrP C is membrane surface glicoprotein, that has important function including antioxidant, antiapoptotic protection, transport of certain ions and others. PrP C conversion into the pathological form leads to changes of the molecule conformation, consequently, to loss its physiological functions with a violation of metabolism in general.The localization of PrP C in the cerebellum of rats of different ages has been demonstrated. The cellular prion was found in the cerebellar cortex, particularly in neurons of the molecular and granular layers, as well as in Purkinje cells. The localization of PrP C was unchanged under age-related changes of the cerebellum. The amount of cellular prion was determined by the immunohistochemical and dot blot analysis methods. In particular, the PrP C level was the highest in cerebellum of six months rats and the lowest in cerebellum of thirty months animals.

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Cellular Prion Protein and Sexual Dimorphic Areas in Rodents. Correlates with Alzheimer Disease

Abstract: The cellular prion protein (PrPC) expression was analyzed by western-blot

Cited by 4 publications

References 48 publications

Physiological prion and activity of plasma membrane Na(+),K(+)- and Ca(2+)-ATPase in the medulla oblongata of rats of different ages

Physiological prion and activity of plasma membrane Na(+),K(+)- and Ca(2+)-ATPase in the medulla oblongata of rats of different ages

Application of capillary immunoelectrophoresis revealed an age‐ and gender‐dependent regulated expression of PrPC in liver

Qualitative analysis of cellular prion in the cerebellum of the wistar line rats of different age groups

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