Cellular profiles in the abomasal mucosa and lymph node during primary infection with Haemonchus contortus in sheep

Balic, Adam; Bowles, Vernon M; Meeusen, Elza Nicole Theresia

doi:10.1016/s0165-2427(00)00189-6

Cited by 93 publications

(90 citation statements)

References 16 publications

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“…It has been confirmed that both mast cells and intraepithelial mucosal mast cells (globule leucocytes) are present in substantial numbers in abomasal tissue niches of hypersensitized and relatively resistant animals. Additionally, mucosal tissue eosinophilia, increased mucus production and the Evaluation of resistance status of small ruminants presence of inhibitory substances in the mucus have been consistently observed in relation to the development of immunity to GINs in ruminants (Huntley et al, 1984 andPfeffer et al, 1996;Harrison et al, 1999;Balic et al, 2000b;Bricarello et al, 2004). Differences in cellular counts in resistant and susceptible individuals/breeds and their possible role as parameters are summarized in the following.…”

Section: Abomasal Histologymentioning

confidence: 96%

“…circumcincta developed in two stages: first, in association with the development of a specific local IgA response that resulted in the reduction of average egg production per worm; and second, in association with the production of globule leucocytes in the abomasal mucosa that resulted in the control of number of worms. In several studies, the number of globule leucocytes was negatively correlated with the number of worms, suggesting that this cell type has a specific role in the resistance of the host (Douch et al, 1986;Stear et al, 1995;Balic et al, 2000b;Gruner et al, 2004). Significant differences in the globule leucocyte counts were also reported in St. Croix (resistant) and Dorset (susceptible) sheep (Gamble and Zajac, 1992) and Crioula Lanada sheep (resistant) and in Corriedale (susceptible) lambs (Bricarello et al, 2004).…”

Section: Mast Cellsmentioning

confidence: 99%

“…Tel. circumcincta infective larvae habitually penetrate and establish deeply in the crypts of the abomasal tissue as compared with H. contortus L3, which prefer more surface niches (Balic et al, 2000b). This mechanism leads to the higher removal of H. contortus L3 from abomasal tissues than that of Tel.…”

Section: Abomasal Histologymentioning

confidence: 99%

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Markers/parameters for the evaluation of natural resistance status of small ruminants against gastrointestinal nematodes

Saddiqi¹,

Sarwar

Iqbal

et al. 2012

Animal

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The high prevalence of anthelmintic-resistant gastrointestinal nematodes (GINs) throughout the world has led to the need for alternative worm control strategies. One of the possible substitutes to reduce the problems of drug resistance and residue is the evaluation/breeding of small ruminants for greater resistance to the GINs (organically produced), which in turn would be a helpful tool to predict the performance of an animal. At present, the existing diversity in the genetic potential to resist/tolerate GINs infection both within and between breeds has been validated. Successful selection of animals to define the genotype and identified resistance is related to the employed markers. A number of phenotypic traits such as faecal egg count (FEC), worm burden, serum antibodies, peripheral eosinophilia, packed cell volume, live weight, serum protein and albumin concentrations have been used for this purpose both in natural and artificial infections. Relatively resistant/tolerant animals have also been found to have mastocytosis, globule leucocytes, high levels of histamine and immunoglobulin (Ig) A and IgE concentrations. Of these traits, the principal and most practical measurement used to assess resistance status in animals undergoing similar parasite challenges is FEC. FEC has a positive/negative correlation with other biochemical, cellular and immunological parameters; however, the reliability of individual trial is often questioned and valuable information regarding the genetic makeup can be obtained from pooled data of a large number of trials and parameters. This paper covers all the aspects reported in the literature on various parameters considered to evaluate the resistance status of a range of small ruminant breeds.

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Section: Abomasal Histologymentioning

confidence: 96%

Section: Mast Cellsmentioning

confidence: 99%

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Markers/parameters for the evaluation of natural resistance status of small ruminants against gastrointestinal nematodes

Saddiqi¹,

Sarwar

Iqbal

et al. 2012

Animal

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“…Effector cells degranulate, releasing vasoactive molecules, which physically expel GINs from the abomasal mucosa [9; 99]. Eosinophils are closely associated with GIN infection, and activated by Th2 cytokines, such as IL-3 and IL-5 [105]. Sheep elecit an early immune response to infection with H. contortus larvae, represented by the CD4 T-cell and B-cell activation in the draining lymph nodes and eosinophil, CD4(+) and gamma delta-TCR,WC1(+) T-cell and B-cell recruitment.…”

Section: Host Response To Haemonchus Infection:-mentioning

confidence: 99%

“…Sheep elecit an early immune response to infection with H. contortus larvae, represented by the CD4 T-cell and B-cell activation in the draining lymph nodes and eosinophil, CD4(+) and gamma delta-TCR,WC1(+) T-cell and B-cell recruitment. However, increases in mast cell numbers prevail the local response during infection with the adult parasite [105]. Mediators released by eosinophils include major basic protein, peroxidases, and neurotoxins, and have been demonstrated to have lethal effects against GIN parasites in vitro [106].…”

Section: Host Response To Haemonchus Infection:-mentioning

confidence: 99%

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Ashram¹,

Nasr²,

Mehmood³

et al. 2017

IJAR

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Gastrointestinal (GI) parasitic infections are a world-wide problem for both small-and large-scale farmers. Infection by GI parasites in ruminants, including sheep and goat can result in harsh economic losses in a variety of ways: reproductive inefficiency, decreased work capacity, involuntary culling, diminished food intake, poor animal growth rates and lower weight gains, treatment and management costs, and mortality in heavily parasitized animals. Among the GI parasites that cause losses to the farming industry, the barber"s pole worm Haemonchus contortus is the predominant, blood-sucking, highly pathogenic, and economically important nematode that infects small ruminants. Here, we review the historical and recent literature on the ovine-parasite-environment interaction for H. contortus to bring avenues where advances in the understanding of these interactions is an indispensible to develop a cost effective control strategies as potential options for the haemonchosis control in sheep and the proper management of sheep in various production systems.

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Experimental infection of Haemonchus contortus strains resistant and susceptible to benzimidazoles and the effect on mast cells distribution in the stomach of Mongolian gerbils (Meriones unguiculatus)

et al. 2007

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Establishment rate of Haemonchus contortus in non-suppressed and immunosuppressed gerbils within 14 days post-infection was compared after inoculation with 1,000 third-stage larvae (L3), exsheathed BZ-susceptible larvae. Based on significantly higher number of larvae in gerbils receiving low doses of immunosuppressant agent hydrocortisone, development of benzimidazole (BZ)-susceptible and BZ-resistant strain of nematode in the stomach was studied on days 4, 7, 10, and 14 p.i. Sections of stomach from both groups of animals were examined for overall histopathological response and dynamics of mucosal mast cells (MMC) and connective tissue mast cells (CTMC). In the immunosuppressed gerbils, H. contortus L3 stage larvae developed to the L4 stage on days 10 and 14 p.i., and their sex ratio was higher toward female worms. Significantly higher ratios of establishment rate were recorded for BZ-susceptible than BZ-resistant strain. Infection elicited strong inflammation mainly in the lamina propria mucosae, where MMC numbers peaked on day 7 p.i., being present in a significantly higher numbers in gerbils infected with BZ-susceptible strain. Infection with BZ-susceptible strain of nematode also resulted in a higher number of CTMC in comparison with the effect of BZ-resistant strain, which were observed in the tela submucosa only. Thus, H. contortus infection in gerbils seems to be a suitable model to study host-parasite interactions. Our results indicate that BZ-resistant strain of H. contortus have a decreased capacity to establish infection in direct relation with lower mucosal and connective tissue MCs counts in the stomach.

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Cellular profiles in the abomasal mucosa and lymph node during primary infection with Haemonchus contortus in sheep

Cited by 93 publications

References 16 publications

Markers/parameters for the evaluation of natural resistance status of small ruminants against gastrointestinal nematodes

Markers/parameters for the evaluation of natural resistance status of small ruminants against gastrointestinal nematodes

Haemonchus Contortus and Ovine Host: A Retrospective Review.

Experimental infection of Haemonchus contortus strains resistant and susceptible to benzimidazoles and the effect on mast cells distribution in the stomach of Mongolian gerbils (Meriones unguiculatus)

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