Cellular properties of human gingival fibroblasts on novel and conventional implant-abutment materials

Rozeik, Ahmed Said; Chaar, Mohamed Sad; Sindt, Sandra; Wille, Sebastian; Selhuber‐Unkel, Christine; Kern, Matthias; El-Kholy, Samar; Dörfer, Christof E.; El‐Sayed, Karim M. Fawzy

doi:10.1016/j.dental.2021.12.139

Cited by 16 publications

(14 citation statements)

References 37 publications

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“…In terms of cell proliferation, the findings of this study showed that HGFB exhibited a better proliferation rate when seeded on m-PEEK and ZrO 2 discs from day 1 to day 10. Similar results were reported in previous studies, when PEEK was modified with plasma spraying and compared with coated and non-coated Ti, Zr, and other materials and surfaces [ 28 , 41 ]. On the other hand, both materials (m-PEEK and ZrO 2 ) exhibited the smallest proliferation percentage at day 10 compared to Ti, TiN, and CoCr, when cultured with HGKC.…”

Section: Discussionsupporting

confidence: 88%

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

et al. 2022

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Objectives: This study aimed to investigate the response of human gingival fibroblasts (HGFB) and human gingival keratinocytes (HGKC) towards different dental implant abutment materials. Methods: Five materials were investigated: (1) titanium (Ti), (2) titanium nitride (TiN), (3) cobalt-chromium (CoCr), (4) zirconia (ZrO2), and (5) modified polyether ether ketone (m-PEEK). Both cell lines were cultured, expanded, and seeded in accordance with the protocol of their supplier. Cell proliferation and cytotoxicity were evaluated at days 1, 3, 5, and 10 using colourimetric viability and cytotoxicity assays. Data were analysed via two-way ANOVA, one-way ANOVA, and Tukey’s post hoc test (p < 0.05 for all tests). Results: There was a statistically significant difference in cell proliferation of HGKC and HGFB cells in contact with different abutment materials at different time points, with no significant interaction between different materials. There was a significant effect on cell proliferation and cytotoxicity with different exposure times (p < 0.0001) for each material. Cell proliferation rates were comparable for both cell lines at the beginning of the study, however, HGFB showed higher proliferation rates for all materials at day 10 with better proliferation activities with ZrO and m-PEEK (40.27%) and (48.38%) respectively. HGKC showed significant interactions (p < 0.0001) in cytotoxicity between different materials. Conclusion: The present in vitro assessment investigated the biocompatibility of different abutment materials with soft tissue cells (HGFB and HGKC). The findings suggest that m-PEEK and TiN are biologically compatible materials with human cells that represent the soft tissue and can be considered as alternative implant abutment materials to Ti and ZrO2, especially when the aesthetic is of concern.

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Section: Discussionsupporting

confidence: 88%

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

et al. 2022

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“…The available evidence suggests that PEEK abutments do not have sufficient biomechanical requirements to replace established titanium abutments [ 67 ], and zirconium remains the most biocompatible abutment material [ 68 ]. However, as mentioned before, PEEK materials offer specific advantages in applications such as temporary restorations in patients without functional impairments and the creation of emergency contours during the surgical phase, especially for implant restorations in patients carrying weak stress loads.…”

Section: Mechanical Properties Of Peek In Dental Applicationsmentioning

confidence: 99%

PEEK for Oral Applications: Recent Advances in Mechanical and Adhesive Properties

et al. 2023

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Polyetheretherketone (PEEK) is a thermoplastic material widely used in engineering applications due to its good biomechanical properties and high temperature stability. Compared to traditional metal and ceramic dental materials, PEEK dental implants exhibit less stress shielding, thus better matching the mechanical properties of bone. As a promising medical material, PEEK can be used as implant abutments, removable and fixed prostheses, and maxillofacial prostheses. It can be blended with materials such as fibers and ceramics to improve its mechanical strength for better clinical dental applications. Compared to conventional pressed and CAD/CAM milling fabrication, 3D-printed PEEK exhibits excellent flexural and tensile strength and parameters such as printing temperature and speed can affect its mechanical properties. However, the bioinert nature of PEEK can make adhesive bonding difficult. The bond strength can be improved by roughening or introducing functional groups on the PEEK surface by sandblasting, acid etching, plasma treatment, laser treatment, and adhesive systems. This paper provides a comprehensive overview of the research progress on the mechanical properties of PEEK for dental applications in the context of specific applications, composites, and their preparation processes. In addition, the research on the adhesive properties of PEEK over the past few years is highlighted. Thus, this review aims to build a conceptual and practical toolkit for the study of the mechanical and adhesive properties of PEEK materials. More importantly, it provides a rationale and a general new basis for the application of PEEK in the dental field.

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“…At least five drops were measured for each graft to obtain the average values of water contact angle. [37] Compliance: Tubular grafts were soaked into PBS solutions for 24 h and then nested with a polyurethane tube. The grafts were fixed to the instrument and measured under at a pressure of 80-120 mmHg.…”

Section: Fabrication Of Tubular Graftsmentioning

confidence: 99%

“…Several studies have revealed that biomaterials with contact angles in the range of 35°-55°facilitated good cell adhesion and proliferation. [35][36][37] Degradation of grafts is also a key factor in determining graft remodeling rate. [38] Previous studies suggested that slow degradation of PVA limited the regeneration of blood vessels.…”

Section: Characterization Of the Pva-bsn Composite Tubular Graftsmentioning

confidence: 99%

Biomimetic Vascular Grafts with Circumferentially and Axially Oriented Microporous Structures for Native Blood Vessel Regeneration

Ding,

Wang,

Chen

et al. 2023

Adv Funct Materials

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Porous grafts facilitate constructive remodeling of blood vessels. Incorporating multiple biomimetic cues to porous grafts can promote vascular regeneration. However, the fabrication of such medical devices remains challenging. Here, beta‐sheet rich silk nanofibers (BSN) are added to poly(vinyl alcohol) (PVA) solution and aggregated under a cylindric electric field to form circumferentially and axially oriented tubular structures, to simulate the endothelial and media layers of blood vessels. PVA in the aligned tubes is then crystallized through repeat freezing–thawing process to offer mechanical performances. Through tuning the ratio of BSN and PVA, the composite tubes with dual anisotropic microstructures exhibit better mechanical properties than pure PVA vascular grafts. Significantly improved cell adhesion, spreading, proliferation, and alignment are achieved. Both endothelial and smooth muscle cells show improved biological activity on the grafts due to the regulatory roles of the aligned structures. In vivo studies reveal the formation of endothelial layers within four weeks of implantation, ensuring long‐term patency. The endothelial and smooth muscle double layers are regenerated after eight months postimplantation, forming hierarchical microstructures and compositions similar to native vessels. The porous composite grafts with multiple aligned structures guide vascular remodeling to regenerate blood vessels, demonstrating potential for clinical translation.

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Cellular properties of human gingival fibroblasts on novel and conventional implant-abutment materials

Cited by 16 publications

References 37 publications

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

PEEK for Oral Applications: Recent Advances in Mechanical and Adhesive Properties

Biomimetic Vascular Grafts with Circumferentially and Axially Oriented Microporous Structures for Native Blood Vessel Regeneration

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