Cellular radiosensitivity of primary head and neck squamous cell carcinomas and local tumor control

Brock, William A.; Baker, Fraser L.; Wike, Jennifer L.; Sivon, Susan L.; Peters, Lester J.

doi:10.1016/0360-3016(90)90298-x

Cited by 94 publications

(20 citation statements)

References 12 publications

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“…The culturing success rate was 74% and SF, was obtained for 63% of all patients biopsied. The latter figure is similar to the 60% success rate reported by Brock et al (1990) for primary head and neck carcinomas using the CAM assay. Girinsky et al (1993) reported a success rate for obtaining SF, values in head and neck cancers using the CAM assay of 75% when the cell yield was high enough to allow cell cultures to be set up.…”

Section: Discussionsupporting

confidence: 88%

“…Using a soft-agar assay our mean value for the radiosensitivity of head and neck cancers was higher than those reported using the CAM assay (Brock et al, 1990;Girinsky et al, 1994) and this probably reflects differences between the two assays. The radiosensitivites of the head and neck cancers studied were variable (SF2 from 0.10 to 1.00).…”

Section: Discussioncontrasting

confidence: 83%

“…tests. However, intrinsic radiosensitivity expressed as parameters of the low-dose region of radiation cell survival curves is thought to be one of the most important and significant factors determining the response of a tumour to radiation treatment (Deacon et al, 1984;Fertil and Malaise, 1985;Tucker and Thames, 1989;Brock et al, 1990Brock et al, , 1992Davidson et al, 1990;West and Hendry 1992;West et al, 1993West et al, , 1997Girinsky et al, 1993Girinsky et al, , 1994.…”

Section: Sf2mentioning

confidence: 99%

“…The cell adhesive matrix (CAM) assay has been used by two groups to evaluate tumour radiosensitivity in head and neck cancers. For patients treated with post-operative radiotherapy, SF2 was not predictive of treatment outcome (Brock et al, 1990(Brock et al, , 1992. However, in a study in which the majority of patients were treated with radiotherapy alone, tumour radiosensitivity was a significant prognostic factor (Girinsky et al, 1993(Girinsky et al, , 1994.…”

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confidence: 99%

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The in vitro radiosensitivity of human head and neck cancers

Björk‐Eriksson

West²,

Karlsson³

et al. 1998

Br J Cancer

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Summary A study was made of the intrinsic radiosensitivity of 140 biopsy and surgical specimens of malignant head and neck tumours of different histologies. Using a soft-agar clonogenic assay, the material was assessed for the ability to grow in culture (colony-forming efficiency; CFE) and inherent tumour radiosensitivity (surviving fraction at 2 Gy, SF2). The success rate for obtaining growth was 74% (104/140) with a mean CFE of 0.093% (median 0.031) and a range of 0.002-1.3%. SF2 was obtained for 88 of 140 specimens, representing a success rate of 63% with a mean SF2 of 0.48 (median 0.43) and a range of 0.10-1.00. There were no significant differences in radiosensitivity between different sites of the head and neck region. There were no significant relationships between SF2 and disease stage, nodal status, tumour grade, patient age, primary tumour growth pattern and CFE. The results were compared with those for other tumour types previously analysed with the same assay. The distribution of the SF2 values for the head and neck tumours was similar to that for 145 cervix carcinomas and there was no significant difference in mean radiosensitivity between the two tumour types. Also, there was no significant difference in radiosensitivity between head and neck tumours and either breast or colorectal cancers. However, a group of eight lymphomas was significantly more radiosensitive. These results confirm the feasibility of carrying out radiosensitivity measurements using a soft-agar clonogenic assay on head and neck tumours. In addition, the work has shown that radiosensitivity is independent of many clinical parameters and that the mean value is similar to that reported for cervix carcinomas.

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Section: Discussionsupporting

confidence: 88%

Section: Discussioncontrasting

confidence: 83%

Section: Sf2mentioning

confidence: 99%

mentioning

confidence: 99%

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The in vitro radiosensitivity of human head and neck cancers

Björk‐Eriksson

West²,

Karlsson³

et al. 1998

Br J Cancer

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“…More recently, it has been shown that this observed clinical variation in radiation response could be explained in part by differences in in vitro sensitivity of cell lines (Malaise et al, 1986). For cervical cancer (West et al, 1993), glioma (Ramsay et al, 1992) and head and neck cancers (Brock et al, 1990) there is also a wide range of in vitro radiosensitivity within each histological group and that this may correlate with clinical response (West et al, 1993). Although melanomas have been classically regarded as radioresistant, there is both clinical data (Harwood and Cummings, 1981) and in vitro data (Rofstad, 1986) to suggest that there is heterogeneity in radiation response.…”

mentioning

confidence: 99%

Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia

Ramsay

Birrell²,

Baumann³

et al. 1998

Br J Cancer

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Summary The human melanoma cell lines MM96L, A2058 and HT1 44 were examined for sensitivity to ionizing radiation and UVB radiation. HT1 44 demonstrated a significant increase in sensitivity to ionizing and UVB radiation compared with the MM96L and A2058 cells. Sensitivity to both agents was associated with susceptibility to apoptosis. Using a protein truncation assay, a mutation for the gene for ataxia telangiectasia (ATM) was identified in HT144 cells. This was confirmed to be a homozygous mutation by subsequent sequencing of the abnormal region. Protein truncation assay of the other two cell lines showed no abnormality. The results suggest that somatic mutation of the A-T gene may be important in determining tumour radiosensitivity.Keywords: melanoma; radiosensitivity; apoptosis; ataxia telangiectasia; ATM gene From the early days of radiotherapy it has been realized that tumours vary in their sensitivity to radiation. In 1936, Paterson divided tumours into three groups: sensitive, intermediate and radioresistant. The first category included embryonic tumours and lymphomas, the second squamous cell and adenocarcinomas and the third gliomas, sarcomas and melanomas. More recently, it has been shown that this observed clinical variation in radiation response could be explained in part by differences in in vitro sensitivity of cell lines (Malaise et al, 1986). For cervical cancer (West et al, 1993), glioma (Ramsay et al, 1992) and head and neck cancers (Brock et al, 1990) there is also a wide range of in vitro radiosensitivity within each histological group and that this may correlate with clinical response (West et al, 1993). Although melanomas have been classically regarded as radioresistant, there is both clinical data (Harwood and Cummings, 1981) and in vitro data (Rofstad, 1986) to suggest that there is heterogeneity in radiation response. In this study, we have examined the mechanism in observed differences in radiosensitivity between three melanoma cell lines. An important observation has been the identification of a homozygous mutation for the gene for ataxia telangiectasia (A-T) in the radiosensitive cell line. A-T is a rare autosomal recessive condition with affected individuals showing hypersensitivity to radiation and predisposition to cancer (Taylor, 1982). Recently, the condition has been found to be caused by mutation of a single gene designated ATM (ataxia telangiectasia mutated) (Savitsky et al, 1995). Although the sensitivity of normal cells from A-T patients has been well characterized, little is known about mutations in tumours and whether this is also associated with radiosensitivity. Received 28 November 1996Revised 2 July 1997 Accepted 8 July 1997 Correspondence to: J Ramsay MATERIALS AND METHODS Cell linesThe established human malignant melanoma lines MM96L, A2058 and HT144 were cultured as monolayers in 5% carbon dioxide/air at 37°C in RPMI-1640 culture medium supplemented with 10% (v/v) fetal calf serum (FCS), penicillin (100 IU ml-'), streptomycin (100 jig ml-') and Hepes (3 mM). Th...

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