Cellular response and extracellular vesicles characterization of human macrophages exposed to fine atmospheric particulate matter

Martin, Perrine; Héliot, Amélie; Trémolet, Gauthier; Landkocz, Yann; Dewaele, Dorothée; Cazier, Fabrice; Ledoux, Frédéric; Courcot, Dominique

doi:10.1016/j.envpol.2019.07.101

Cited by 39 publications

(28 citation statements)

References 53 publications

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“…In addition, PM exposure can trigger inflammatory response [32], worsen chronic conditions spread all over the body sites, and also heighten the risk for acute and chronic diseases [33][34][35][36][37][38]. Several epidemiological studies showed how the effects exerted by PM exposure on human health can be attributed, at least in part, to plasmatic EV modifications, which include variations in the transcriptomic and proteomic content, as well as in the circulating amount of different EV types [39][40][41][42][43]. In addition, documentation shows that PM exposure may trigger EV release in a dose-dependent manner [41], inducing the release of proinflammatory cytokines such as IL6 and TNF-α [44].…”

Section: Discussionmentioning

confidence: 99%

Nasal Microbiota Modifies the Effects of Particulate Air Pollution on Plasma Extracellular Vesicles

Mariani

Favero

Carugno

et al. 2020

IJERPH

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Air pollution exposure has been linked to modifications of both extracellular vesicle (EV) concentration and nasal microbiota structure (NMB), which might act as the respiratory health gatekeeper. This study aimed to assess whether an unbalanced NMB could modify the effect of particulate matter (PM) exposure on plasmatic EV levels. Due to two different NMB taxonomical profiles characterized by a widely different relative abundance of the Moraxella genus, the enrolled population was stratified into Mor− (balanced NMB) and Mor+ (unbalanced NMB) groups (Moraxella genus’s cut-off ≤25% and >25%, respectively). EV features were assessed by nanoparticle tracking analysis (NTA) and flow-cytometry (FC). Multivariable analyses were applied on EV outcomes to evaluate a possible association between PM10 and PM2.5 and plasmatic EV levels. The Mor− group revealed positive associations between PM levels and plasmatic CD105+ EVs (GMR = 4.39 p = 0.02) as for total EV count (GMR = 1.92 p = 0.02). Conversely, the Mor+ group showed a negative association between exposure and EV outcomes (CD66+ GMR = 0.004 p = 0.01; EpCAM+ GMR = 0.005 p = 0.01). Our findings provide an insight regarding how a balanced NMB may help to counteract PM exposure effects in terms of plasmatic EV concentration. Further research is necessary to understand the relationship between the host and the NMB to disentangle the mechanism exerted by inhaled pollutants in modulating EVs and NMB.

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Section: Discussionmentioning

confidence: 99%

Nasal Microbiota Modifies the Effects of Particulate Air Pollution on Plasma Extracellular Vesicles

Mariani

Favero

Carugno

et al. 2020

IJERPH

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“…Available studies about MDM-derived sEVs (Table 3) have applied different procedures for their description, were mainly aimed to functional approaches and not to methodological improvements, or evaluated multiple proteins or microRNA contents from a heterogeneous population of vesicles secreted by macrophages. In fact, some of these works used large volume of culture supernatant or even additional isolation steps before the final centrifugation (e.g., filtration) [17,18,[64][65][66][67][68][69][70][71][72]. In contrast, we suggest here a modified protocol based on sucrose density gradient ultracentrifugation with higher speeds that allowed by itself the separation and quantification of large amounts of small EVs enriched with vesicles from the endosomal origin, which is consistent with results reported by other authors [51], who showed that dUC with increasing speed pelleted EVs with decreasing sizes.…”

Section: Plos Onementioning

confidence: 99%

Characterization and internalization of small extracellular vesicles released by human primary macrophages derived from circulating monocytes

et al. 2020

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Extracellular vesicles (EVs) are small membrane-limited structures derived from outward budding of the plasma membrane or endosomal system that participate in cellular communication processes through the transport of bioactive molecules to recipient cells. To date, there are no published methodological works showing step-by-step the isolation, characterization and internalization of small EVs secreted by human primary macrophages derived from circulating monocytes (MDM-derived sEVs). Thus, here we aimed to provide an alternative protocol based on differential ultracentrifugation (dUC) to describe small EVs (sEVs) from these cells. Monocyte-derived macrophages were cultured in EV-free medium during 24, 48 or 72 h and, then, EVs were isolated from culture supernatants by (dUC). Macrophages secreted a large amount of sEVs in the first 24 h, with size ranging from 40-150 nm, peaking at 105 nm, as evaluated by nanoparticle tracking analysis and scanning electron microscopy. The markers Alix, CD63 and CD81 were detected by immunoblotting in EV samples, and the co-localization of CD63 and CD81 after sucrose density gradient ultracentrifugation (S-DGUC) indicated the presence of sEVs from late endosomal origin. Confocal fluorescence revealed that the sEVs were internalized by primary macrophages after three hours of co-culture. The methodology here applied aims to contribute for enhancing reproducibility between the limited number of available protocols for the isolation and characterization of MDM-derived sEVs, thus providing basic knowledge in the area of EV methods that can be useful for those investigators working with sEVs released by human primary macrophages derived from circulating monocytes.

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“…AMs are directly exposed to environmental antigens and particulate matter (PM). Exposure to PM induces the release of EVs in a dose-dependent manner, and the PM-induced EVs exert a pro-inflammatory phenotype on pulmonary epithelial cells, resulting in the release of the pro-inflammatory cytokines Chemotaxis ↑ 35 pro-inflammatory (TN-C) 38 Th2 polarization (miRNA-92b, miR-210 and miR-34a↓) 36 Chemotaxis ↑ (S100 A12) 42 Smooth muscle Fibroblasts Proliferation ↑ (TGF-β2↓) 45 Inhibition of myofibroblast differentiation (PGE 2 ) 46 Macrophages -Dampening of inflammation (SOCS1, SOCS3) 52,53 -proinflammatory IL-6 and TNF-α↑ 55 -ICAM1 expression and cytokine secretion ↑ (TNFα) 61 Enzymes for biosynthesis of leukotrienes 64 -Differentiation of monocytes into macrophages↑(miR-223) 57 -after bacterial infection: TNF-α secretion ↑ (bacterial PAMPs) 60 -Migration (chemotactic eicosanoids) 64 -TNF-α secretion ↑ (bacterial PAMPs) 60 Ag presentation via MHCII and Th2 polarization 58 Gelatinolytic and collagenolytic activity (MMP-14) 56 Dendritic cells IL-6 and tumor necrosis factor α (TNF-α) (55). EVs derived from CSE-exposed macrophages have been shown to contain MMP-14 with gelatinolytic and collagenolytic activity and might, therefore, be involved in emphysema development in COPD (56).…”

Section: Macrophagesmentioning

confidence: 99%

Section: Structural Cellsmentioning

confidence: 99%

Extracellular Vesicles as Mediators of Cellular Cross Talk in the Lung Microenvironment

et al. 2020

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The human lung is a complex tissue subdivided into several regions that differ in size, function, and resident cell types. Despite years of intensive research, we still do not fully understand the cross talk between these different regions and diverse cell populations in the lung and how this is altered in the development of chronic respiratory disease. The discovery of extracellular vesicles (EVs), small membrane vesicles released from cells for intercellular communication, has added another layer of complexity to cellular cross talk in the complex lung microenvironment. EVs from patients with chronic obstructive pulmonary disease, asthma, or sarcoidosis have been shown to carry microRNAs, proteins, and lipids that may contribute to inflammation or tissue degeneration. Here, we summarize the contribution of these small vesicles in the interplay of several different cell types in the lung microenvironment, with a focus on the development of chronic respiratory diseases. Although there are already many studies demonstrating the adverse effects of EVs in the diseased lung, we still have substantial knowledge gaps regarding the concrete role of EV involvement in lung disease, which should be addressed in future studies.

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Cellular response and extracellular vesicles characterization of human macrophages exposed to fine atmospheric particulate matter

Cited by 39 publications

References 53 publications

Nasal Microbiota Modifies the Effects of Particulate Air Pollution on Plasma Extracellular Vesicles

Nasal Microbiota Modifies the Effects of Particulate Air Pollution on Plasma Extracellular Vesicles

Characterization and internalization of small extracellular vesicles released by human primary macrophages derived from circulating monocytes

Extracellular Vesicles as Mediators of Cellular Cross Talk in the Lung Microenvironment

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