Cellular response in semi-intensively cultured sea bream gills to Ergasilus sieboldi (Copepoda) with emphasis on the distribution, histochemistry and fine structure of mucous cells

Dezfuli, Bahram Sayyaf; Squerzanti, Samantha; Fabbri, S.; Castaldelli, Giuseppe; Giari, Luisa

doi:10.1016/j.vetpar.2010.08.024

Cited by 21 publications

(10 citation statements)

References 54 publications

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“…At infection of the see bream with Ergasilus sieboldi Nordmann, 1832. Dezfuli, Squerzanti, Fabbri, Castaldelli, and Giari () and Dezfuli, Giari, Lui, Lorenzoni, and Noga () besides hypertrophy of epithelioid cells observed haemorrhage, increase of rodlet cells, mast cells and mucous cells adjacent to the attachment site of copepods. Similar changes were caused by Sinergasilus lieni Yin, 1949 on silver carp in Hungary when besides the above mention signs, clubbing, fusion of neighbouring filaments were recorded (Molnár & Székely, ).…”

Section: Discussionmentioning

confidence: 92%

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Histopathological changes on the gills of asp (Aspius aspius) and European catfish (Silurus glanis) caused by Lamproglena pulchella and a Lamproglena sp. (Copepoda: Lernaeidae), respectively

Molnár

Avenant‐Oldewage

Sellyei

et al. 2017

Journal of Fish Diseases

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In a parasitology survey of Hungarian fishes, heavy infections of parasitic copepods Lamproglena pulchella and a Lamproglena sp. were found in the gills of the asp and the European catfish, respectively. Individuals of both fish species were emaciated and infected with hundreds of Lamproglena. Copepods located close to the tip of gill filaments and formed a depression at the attachment sites. In histological sections, cell degenerations and local haemorrhages were present adjacent to the maxillipeds and where the maxillary claws pierced the gill tissue. Around maxillae and in the midgut of the Lamproglena, damaged piscine blood cells and remains of the gill tissue were observed. Host reaction was expressed by proliferation of epithelioid cells, increase in both number and size of goblet and mast cells and formation of giant cells.

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Section: Discussionmentioning

confidence: 92%

“…At infection of the see bream with Ergasilus sieboldi Nordmann, 1832. Dezfuli, Squerzanti, Fabbri, Castaldelli, and Giari (2010) and Dezfuli, Giari, Lui, Lorenzoni, and Noga (2011) Both the asp and European catfish are precious sport fishes.…”

Section: Discussionmentioning

confidence: 99%

Histopathological changes on the gills of asp (Aspius aspius) and European catfish (Silurus glanis) caused by Lamproglena pulchella and a Lamproglena sp. (Copepoda: Lernaeidae), respectively

Molnár

Avenant‐Oldewage

Sellyei

et al. 2017

Journal of Fish Diseases

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“…Its more abundant secretion in cultured fish imposes unequivocally more pressure on the parasite. Likewise, in cultured sea bream infected with copepod Ergasilus sieboldi , mucus cells encircling the parasite were significantly more abundant in infected than uninfected tissue [57], which, along with immunological properties of gill mucus, suggests active involvement in parasite rejection. We suggest that such host mechanism of increased mucus secretion induces changes to longer haptoral elements in Furnestinia infecting reared sea bream compared to wild fish.…”

Section: Discussionmentioning

confidence: 92%

Morphological Plasticity and Phylogeny in a Monogenean Parasite Transferring between Wild and Reared Fish Populations

Mladineo

Šegvić-Bubić

Stanić³

et al. 2013

PLoS ONE

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It is widely accepted that disease interactions between cultured and wild fish occur repeatedly, although reported cases have mainly relied just on the observation of similar symptoms in affected populations. Whether there is an explicit pathogen transfer between fish stocks, or each develops its own pathogen population, has been insufficiently studied and rarely supported by molecular tools. In this study, we used population dynamics and genetic structure of the monogenean Furnestinia echeneis in reared and neighbouring wild sea bream to indicate pathogen transfer, characterized by the phenotypic plasticity of the parasite attachment apparatus and the lack of phylogenetic differentiation. The observed pattern of genetic variation inferred by nuclear DNA Internal Transcribed Spacer 1 (ITS1) and mtDNA cytochrome C oxidase 1 (COI), between parasite populations is most likely caused by a recent shared demographic history like a reduced species area in the last glacial period. In spite of such recent expansion that populations underwent, F. echeneis shows differentiation in haptor morphometry as an adaptive trait in closely related populations at the aquaculture site. This suggests that differentiation in morphology may occur relatively rapidly in this species and that adaptive forces, not the speciation process, drives this monogenean parasitation. On the other hand, the observed phylogenetic inertia suggests a low to moderate gene flow (based on FST) between parasites in cultured and wild fish, evidencing for the first time the transfer of pathogens at the aquaculture site inferred by a molecular tool.

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“…MCs play an important role in responding to inflammation [41] and their numbers increase as a consequence of helminth infection in fish intestine [20, 27, 55] as well as in parasitized liver [13, 22, 56]. Numerous MCs were noticed to be in close contact with capillaries and the outer layer of the endothelia as well as within the lumen of the blood vessels in an infected organ [27, 57, 58]. Similar findings were found in liver and pancreas of Phoxinus phoxinus infected with larvae of the nematode Raphidascaris acus [13], consistent with G. inaequilabiatus liver harbouring Brevimulticaecum sp.…”

Section: Discussionmentioning

confidence: 99%

Nematode infection in liver of the fish Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae) from the Pantanal Region in Brazil: pathobiology and inflammatory response

et al. 2016

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BackgroundA survey on endoparasitic helminths from freshwater fishes in the Pantanal Region (Mato Grosso do Sul, Brazil) revealed the occurrence of third-larval stage of the nematode Brevimulticaecum sp. (Heterocheilidae) in most organs of Gymnotus inaequilabiatus (Gymnotidae) also known by the local name tuvira. The aim of the present study was to examine Brevimulticaecum sp.-infected tuvira liver at the ultrastructural level and clarify the nature of granulomas and the cellular elements involved in the immune response to nematode larvae.MethodsThirty-eight adult specimens of tuvira from Porto Morrinho, were acquired in January and March 2016. Infected and uninfected liver tissues were fixed and prepared for histological and ultrastructure investigations.ResultsThe prevalence of infection of tuvira liver by the nematode larvae was 95 %, with an intensity of infection ranging from 4 to 343 larvae (mean ± SD: 55.31 ± 73.94 larvae per liver). In livers with high numbers of nematode larvae, almost entire hepatic tissue was occupied by the parasites. Hepatocytes showed slight to mild degenerative changes and accumulation of pigments. Parasite larvae were surrounded by round to oval granulomas, the result of focal host tissue response to the infection. Each granuloma was typically formed by three concentric layers: an outer layer of fibrous connective tissue with thin elongated fibroblasts; a middle layer of mast cells entrapped in a thin fibroblast-connective mesh; and an inner layer of densely packed epithelioid cells, displaying numerous desmosomes between each other. Numerous macrophage aggregates occurred in the granulomas and in the parenchyma.ConclusionsOur results in tuvira showed that the larvae were efficiently sequestered within the granulomas, most of the inflammatory components were confined within the thickness of the granuloma, and the parenchyma was relatively free of immune cells and without fibrosis. Presumably this focal encapsulation of the parasites permits uninfected portions of liver to maintain its functions and allows the survival of the host.

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Cellular response in semi-intensively cultured sea bream gills to Ergasilus sieboldi (Copepoda) with emphasis on the distribution, histochemistry and fine structure of mucous cells

Cited by 21 publications

References 54 publications

Histopathological changes on the gills of asp (Aspius aspius) and European catfish (Silurus glanis) caused by Lamproglena pulchella and a Lamproglena sp. (Copepoda: Lernaeidae), respectively

Histopathological changes on the gills of asp (Aspius aspius) and European catfish (Silurus glanis) caused by Lamproglena pulchella and a Lamproglena sp. (Copepoda: Lernaeidae), respectively

Morphological Plasticity and Phylogeny in a Monogenean Parasite Transferring between Wild and Reared Fish Populations

Nematode infection in liver of the fish Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae) from the Pantanal Region in Brazil: pathobiology and inflammatory response

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