Cellular responses of histatin-derived peptides immobilized titanium surface using a tresyl chloride-activated method

Abstract

Effects of histatin-derived peptides immobilization by tresyl chloride-activation technique for MC3T3-E1 cellular responses on titanium (Ti) were evaluated. MC3T3-E1 were cultured on sandblasted and acid-etched Ti disks immobilized with histatin-derived peptides, including histatin-1, JH8194, and mixed histatin-1 with JH8194. Surface topography and cellular morphology were examined using a scanning electron microscope. Elemental composition and conformational peptides on Ti surface were examined using energy dispersive X-ray and fourier transform infrared spectroscopy, respectively. Cellular adhesion, proliferation, osteogenesisrelated genes, and alkaline phosphatase activity were evaluated. The results showed that peptides were successfully immobilized on Ti surface. Cell attachments on histatin-1 and mixed peptides coated groups are higher than control. Histatin-1 achieved the significantly highest cellular proliferation. Histatin-derived peptides improved the osteogenesis related-gene expression and alkaline phosphatase activity (p<0.05). This study suggested that histatin-1 immobilization by tresyl chloride-activation technique enhanced cellular responses and might be able to promote cellular activities around the dental implants.

Role of chitosan in titanium coatings. trends and new generations of coatings

“…Makihira et al tested the performance of Ti implants coated with a histatin-derived peptide on edentulous ridges of dogs; histological analysis and microcomputed tomography showed increased trabecular bone formation around the coated implants versus untreated implants (Makihira et al, 2011). Siwakul et al (Siwakul et al, 2021) evaluated cell adhesion, proliferation, osteogenesis-related genes and alkaline phosphatase activity on histatin-coated Ti surfaces; the results showed that the adhesion of cells to the histatin-coated group achieved cell proliferation significantly histatin achieved significantly higher cell proliferation.…”

Nano-topography and functionalization with the synthetic peptoid GN2-Npm9 as a strategy for antibacterial and biocompatible titanium implants

“…AMPs are often conjugated with metal biomaterials, particularly titanium, to impart antibacterial properties to implant surfaces. Functionalization of AMPs on titanium surfaces can be achieved through various methods, such as covalent bonding [ [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [28] , [28] , [29] , [30] ], or physical adsorption [ [31] , [32] , [33] ]. Once the AMPs are functionalized on the titanium surface, they can provide a potent antimicrobial barrier against bacterial infections.…”

Effectiveness of biomolecule-based bioactive surfaces, on os-seointegration of titanium dental implants: A systematic review and meta-analysis of in vivo studies

“…Despite the existence in the literature of reviews to evaluate the effects of different implant surface modifications on peri-implant bone formation and osseointegration (Makihira et al, 2011;Andrea et al, 2018b;Moussa and Aparicio, 2020;Siwakul et al, 2021) and the known benefit on osseointegration of the use of bioactive molecules (Junker et al, 2009), we have not found meta-analyses that investigate the results in depth, so the aim of our study was to evaluate the role and efficacy of bioactive surfaces on osseointegration. Our meta-analysis limited the research interest to titanium dental implants coated with biomolecules, i.e.…”

“…Makihira et al tested in edentulous dog mandibles, the osseointegrative capacity of Ti implants coated with a histatin-derived peptide, demonstrating, by histological and micro-CT analysis, increased trabecular bone formation around the coated implants ( Riool et al, 2017 ). Their observations suggest that antimicrobial peptides on Ti implants would decrease bacterial colonization on the implant surface and facilitate osseointegration ( Silva et al, 2016 ; Zhang et al, 2018 ).…”