Cellular Temperature Measurement by Dielectrophoretic Impedance Measurement Method

Abstract

Dielectrophoretic impedance measurement (DEPIM) method has recently attracted attention because the method is simple and immediately. We demonstrated that the impedance between the electrodes with trapped cell has thermal property and measured cellular temperature by the impedance change even if the number of trapped cells did not change. However, this result was not observed when cells were not trapped between the electrodes. In summary, the cellular temperature could be measured by observing the change in shunt voltage. In this study, we developed cellular temperature measurement system and it was expected to be used in biosensor application. In the future, we want to measure single cellular temperature with isolation by optical tweezers.

Cellular Thermal Measurement by Dielectrophoresis and the Impedance Changing between Electrodes

“…We measured shunt voltage as the impedance change between the electrodes. It is the same way as previously method about the observing method on electrodes, electric circuits, and analyser [5]. However, we measured by changing the pattern of heating and the distance of the electrodes gap.…”

“…Cellular temperature measurement shows promise for the new way to diagnose cancer and effective hyperthermia therapy. Also, we have already demonstrated to measure cellular thermal changes by dielectrophoresis [5]. It can be precisely measured through measuring the impedance Manuscript received January 6, 2015; revised July 28, 2015.…”