Cellular Trajectories of Peptide-Modified Gold Particle Complexes:  Comparison of Nuclear Localization Signals and Peptide Transduction Domains

Ткаченко, А. Г.; Xie, Haibo; Li, Yanli; Coleman, Donna M.; Ryan, Joseph A.; Glomm, Wilhelm R.; Shipton, Mathew K; Franzen, Stefan; Feldheim, Daniel L.

doi:10.1021/bc034189q

Cited by 429 publications

(399 citation statements)

References 22 publications

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“…[6,7] AuN were used as markers for tracking signal transduction in cells, which also suggests their non-toxic nature. [8,9] The AuN of diameter of 13 nm, covered by oligonucleotides were used for gene regulation. [10] Bulk gold is widely regarded as 'safe' and chemically inert, and, produced as drugs, have been used, inter alia, in the treatment of rheumatic diseases already in the seventies of the twentieth century.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Barbasz

Oćwieja

2015

Journal of Experimental Nanoscience

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Gold nanoparticles (AuN) are one of the most investigated nanomaterials, finding numerous applications from medicine to industry. AuN were obtained by reduction of gold salts using tannic acid as a reducing agent. To detach the impact of AuN onto cells of two lines human monocytic cells (U-937) and human promyelocytic leukaemia cells (HL-60), the effects of postreaction residues (of effluent from sol cleaning) and gold ions were also examined. It was demonstrated that resistance of cells to the toxic action of AuN is dependent not only on incubation time and dosage, but also on stages of cell differentiation. It was found that after incubation of promonocytes U-937 with 25 ppm AuN, the cell viability decreased by 25% and of macrophages by 50%. Differentiated cells U-937 showed a significantly lower resistance than the not-differentiated cells. For HL-60 cells, regardless of differentiation, cell viability decreased by approximately 20% after treatment with 25 ppm AuN sol. It was noticed that U-937 exhibited higher vulnerability to AuN than HL-60 cells. It was proved that AuN induced over a 15-fold increase in nitric oxide and a decrease of intracellular glutathione levels indicating the inflammatory response of cells. Even though gold ions did not show a significant effect on cell viability, they caused fivefold increase of nitric oxide level. The results show a higher cytotoxicity of AuN than gold ions. An overall picture of the interaction of AuN with human cells of first defence line was obtained. The results indicate that AuN may cause changes in the response of phagocytes in inflammatory conditions.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Barbasz

Oćwieja

2015

Journal of Experimental Nanoscience

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“…A severe increase in cell death has only been observed in particles of a diameter of 1.4 and 1.2 nm, which can be regarded as gold atom clusters rather then nanoparticles (15). However, a low-level toxicity was reported in several cell lines after using nanoparticles of various sizes and in different concentrations (9,10,16). Most toxicity studies as yet have used chemically generated GNPs, which need a surface coating to avoid aggregation or are conjugated to ligands like residual reduction agents and additives.…”

Section: Introductionmentioning

confidence: 99%

Nonendosomal cellular uptake of ligand‐free, positively charged gold nanoparticles

et al. 2010

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Gold nanoparticles (GNPs) have interesting optical properties, such as exceptionally high quantum yields and virtually limitless photostability. Therefore, they show the potential for applications as biomarkers especially suitable for in vivo and long-term studies. The generation of GNPs using pulsed laser light rather than chemical means provides nanoparticles, which are remarkably stable in a variety of media without the need of stabilizing agents or ligands. This stabilization is achieved by partial oxidation of the gold surface resulting in positively charged GNPs. However, little is known about cellular uptake of such ligand-free nanoparticles, their intracellular fate, or cell viability after nanoparticle contact. The current work is aimed to explore the response of a bovine cell line to GNP exposure mainly using laser scanning confocal microscopy (LSCM) supported by other techniques. Cultured bovine immortalized cells (GM7373) were coincubated with GNP (average diameter 15 nm, 50 lM Au) for 2, 24, and 48 h. The detection of GNP-associated light scattering by the LSCM facilitated a clear distinction between GNP-containing cells and the negative controls. After 48 h, 75% of cells had visibly incorporated nanoparticles. No colocalization was detected with either Rab5a or Lamp1-positive structures, i.e., endosomes or lysosomes, respectivley. However, transmission electron microscope analysis of GNP-coincubated cells indicated the nanoparticles to be positioned within electron-dense structures. Coincubation at 48C did not inhibit nanoparticle uptake, suggesting diffusion as possible entrance mechanism. Although the assessment of cell morphology, membrane integrity, and apoptosis revealed no GNP-related loss of cell viability at a gold concentration of 25 lM or below, a cytotoxic effect was observed in a proliferation assay after exposing low cell numbers to 50 lM Au and above. In conclusion, this study confirmed the cellular uptake of ligand-free gold nanoparticles during coincubation apparently without using endocytic pathways. '

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“…Gold nanoparticles' exposure at 150 pm for 3 h on HeLa and 3T3/NIH cells resulted in cell viability reduction by 20 and 5%, respectively [153]. In another study, gold nanoparticles were exposed to macrophage cells with particle size range 10-100 µm for 24-72 h. The study demonstrated that the 100 µm nanoparticles decreased the cell viability to 85% in 72 h [154].…”

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confidence: 96%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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Cellular Trajectories of Peptide-Modified Gold Particle Complexes: Comparison of Nuclear Localization Signals and Peptide Transduction Domains

Cited by 429 publications

References 22 publications

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Gold nanoparticles and ions – friends or foes? As they are seen by human cells U-937 and HL-60

Nonendosomal cellular uptake of ligand‐free, positively charged gold nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

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