Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

Liu, Hui; Wang, Jingjing; Li, Wenchao; Hu, Jie; Wang, Min; Kang, Yuejun

doi:10.3390/pharmaceutics10030099

Cited by 5 publications

(3 citation statements)

References 42 publications

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“… 26 Liu and colleagues previously studied the role of nanoparticle rigidity on cellular uptake using G5 dendrimers as soft nanoparticles and dendrimers-coated gold nanoparticles as rigid nanoparticle models. 27 They found that the uptake of dendrimer-coated gold nanoparticles was 3.2-fold higher than that observed from dendrimers in both U87MG cancer cells and L929 healthy cells. As the adsorption and cellular uptake of nanoparticles depend on their shape, surface modifications, size and charge, the enhanced cellular uptake of the gold conjugates could also be due to the cationic polymer modification on the gold nanocages and the positive charge of gold complexes.…”

Section: Discussionmentioning

confidence: 97%

Lactoferrin- and Dendrimer-Bearing Gold Nanocages for Stimulus-Free DNA Delivery to Prostate Cancer Cells

Almowalad¹,

Laskar²,

Somani³

et al. 2022

IJN

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Background The use of gene therapy to treat prostate cancer is hampered by the lack of effective nanocarriers that can selectively deliver therapeutic genes to cancer cells. To overcome this, we hypothesize that conjugating lactoferrin, a tumor-targeting ligand, and the diaminobutyric polypropylenimine dendrimer into gold nanocages, followed by complexation with a plasmid DNA, would enhance gene expression and anti-proliferation activity in prostate cancer cells without the use of external stimuli. Methods Novel gold nanocages bearing lactoferrin and conjugated to diaminobutyric polypropylenimine dendrimer (AuNCs-DAB-Lf) were synthesized and characterized. Following complexation with a plasmid DNA, their gene expression, cellular uptake and anti-proliferative efficacies were evaluated on PC-3 prostate cancer cells. Results AuNCs-DAB-Lf was able to complex DNA at conjugate: DNA weight ratios 5:1 onwards. Gene expression was at its highest after treatment with AuNCs-DAB-Lf at a weight ratio of 10:1, as a result of a significant increase in DNA uptake mediated by the conjugate at that ratio in PC-3 cells. Consequently, the anti-proliferative activity of AuNCs-DAB-Lf-DNA encoding TNFα was significantly improved by up to 9-fold compared with DAB dendriplex encoding TNFα. Conclusion Lactoferrin-bearing dendrimer-conjugated gold nanocages are highly promising gene delivery systems for the treatment of prostate cancer.

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Section: Discussionmentioning

confidence: 97%

Lactoferrin- and Dendrimer-Bearing Gold Nanocages for Stimulus-Free DNA Delivery to Prostate Cancer Cells

Almowalad¹,

Laskar²,

Somani³

et al. 2022

IJN

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“…PAMAM dendrimers, which were the first commercial dendrimers with structures resembling those of proteins, had distinct dendritic structures and three-dimensional spherical shapes. PAMAM dendrimers have been employed extensively in the field of biomedical nanotechnology because of their excellent water solubility, monodispersity, tuneable molecular size, non-immunogenicity, capacity to traverse biological barriers, and ease of functionalisation [ 99 , 100 , 101 ]. PAMAM zero generation (G0) dendrimers were investigated as nanocarriers for drug administration, and conjugated G0 PAMAM dendrimers incorporated with a zinc phthalocyanine (ZnPc) photosensitiser were investigated for their effects on the sick and healthy tissues removed from human carotid arteries.…”

Section: Nanoparticles For Diagnosis and Treatment Of Atherosclerosismentioning

confidence: 99%

Nanoparticles as Drug Delivery Systems for the Targeted Treatment of Atherosclerosis

Pang,

Dinesh,

Pang

et al. 2024

Molecules

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Atherosclerosis continues to be a leading cause of morbidity and mortality globally. The precise evaluation of the extent of an atherosclerotic plaque is essential for forecasting its likelihood of causing health concerns and tracking treatment outcomes. When compared to conventional methods used, nanoparticles offer clear benefits and excellent development opportunities for the detection and characterisation of susceptible atherosclerotic plaques. In this review, we analyse the recent advancements of nanoparticles as theranostics in the management of atherosclerosis, with an emphasis on applications in drug delivery. Furthermore, the main issues that must be resolved in order to advance clinical utility and future developments of NP research are discussed. It is anticipated that medical NPs will develop into complex and advanced next-generation nanobotics that can carry out a variety of functions in the bloodstream.

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“… 5 , 6 For example, the particle shape has been reported to affect the intracellular uptake of the NPs and their biodistribution. 7 Additionally, the particle size 8 , 9 and the particle rigidity 10 , 11 play important roles. Active targeting, which employs ligands with a specific affinity towards the target site, has long been regarded as the optimum strategy for maximizing the tumor accumulation of NPs.…”

Section: Introductionmentioning

confidence: 99%

<p>Distinct Proteins in Protein Corona of Nanoparticles Represent a Promising Venue for Endogenous Targeting – Part II: In vitro and in vivo Kinetics Study</p>

Sebak

Gomaa

ElMeshad

et al. 2020

IJN

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Introduction: Nanoparticles (NPs), upon introduction to the biological systems, become wrapped by serum and cellular proteins constituting the protein corona (PC). This PC contributes largely to the NPs' interaction with the biological systems and their subsequent functions. On the one hand, PC can decrease the efficiency of targeting by directing the NPs to the reticuloendothelial system (RES) or by masking the active targeting moieties and decreasing their ability to bind to their target receptors. On the other hand, some components of PC have offered hopes for achieving endogenous targeting. Methods: In this study, we aimed at the investigation of the role of the PC in determining the behavior of cRGDyk peptide-unconjugated and-conjugated NPs (uNPs and cNPs) exhibiting different physicochemical properties and their interaction with melanoma on in vitro and in vivo levels. Mathematical modeling has been utilized to understand the kinetics of the interaction of NPs with the tumor cells and different organs, respectively. Results: Endocytosis and exocytosis were reported to occur simultaneously for the utilized NPs. The balance was largely dependent on the NPs' physicochemical properties and the role of the PC. In addition, distinct proteins present in the PC (illustrated in the results of the PC analysis in part I) have also determined the patterns of the NPs' distribution in different organs and tissues of the vascular system, the RES system and the target tumot tissue. Vitronectin (VN) was found to mediate higher accumulation in integrin receptor-expressing melanoma cells, while complement 3 protein (C3) and clusterin (CLU), as an opsonin and dysopsonin, respectively, regulated the balance between the RES uptake and blood circulation. Discussion: PC, if properly modulated by tuning NPs' physicochemical properties, can serve as a potential venue for optimum utilization of NPs in cancer therapy.

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Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

Cited by 5 publications

References 42 publications

Lactoferrin- and Dendrimer-Bearing Gold Nanocages for Stimulus-Free DNA Delivery to Prostate Cancer Cells

Lactoferrin- and Dendrimer-Bearing Gold Nanocages for Stimulus-Free DNA Delivery to Prostate Cancer Cells

Nanoparticles as Drug Delivery Systems for the Targeted Treatment of Atherosclerosis

<p>Distinct Proteins in Protein Corona of Nanoparticles Represent a Promising Venue for Endogenous Targeting – Part II: In vitro and in vivo Kinetics Study</p>

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