Cellular Uptake of Carotenoid-Loaded Oil-in-Water Emulsions in Colon Carcinoma Cells in Vitro

Abstract

Oil-in-water emulsions allow the preparation of lipophilic compounds such as carotenoids in the liquid form. Here, the effect of a combination of some emulsifiers, such as two whey protein isolates (BiPro and BioZate), sucrose laurate (L-1695), and polyoxyethylene-20-sorbitan-monolaurate (Tween 20), on the stability of lycopene and astaxanthin in emulsions, droplet size, and cellular uptake of these carotenoids has been investigated. The degradation of lycopene was slightly more pronounced than that of astaxanthin in all emulsions. The concentration of lycopene and astaxanthin decreased by about 30% and 20%, respectively, in all emulsions after 3 weeks of storage in the dark at 4 degrees C. The kind of emulsifiers or their combinations have played an important role in the cellular uptake by the colon carcinoma cells line HT-29 and Caco-2.

Colloidal astaxanthin: Preparation, characterisation and bioavailability evaluation

“…The results also showed that, unlike proteins, the incorporation of astaxanthin in polysaccharide stabilizers caused a decrease in their cellular uptake. These findings were also in good agreement with the results reported by Ribeiro et al (2006).…”

Submicron complex lipid carriers for curcumin delivery to intestinal epithelial cells: Effect of different emulsifiers on bioaccessibility and cell uptake

“…However, recent advancements in drug delivery have shown that the internalization of therapeutics needs to be augmented also by active transport mechanisms, and therefore not only carrier size and charge, but also elasticity and surface composition are important (Agarwal and Roy, 2013). In this respect, previous literature studies demonstrated that polysorbate 20 exhibits good ability to attach on the membrane of intestinal cells like HT29 cell line with an efficient cell penetration capacity (Ribeiro et al, 2006).…”

Inhibition of β-carotene degradation in oil-in-water nanoemulsions: Influence of oil-soluble and water-soluble antioxidants

“…Previously, homogenisation has been used to prepare oil-in-water emulsions or nanoemulsions containing carotenoids, such as lycopene (Boon et al, 2009;Ribeiro, Ax, & Schubert, 2003), lutein (Losso, Khachatryan, Ogawa, Godber, & Shih, 2005) and b-carotene (Chu, Ichikawa, Kanafusa, & Nakajima, 2007Silva et al, 2010). The bioavailability of carotenoids encapsulated within emulsions or nanoemulsions has been reported to be increased when compared to non-encapsulated carotenoids (Grolier, Agoudavi, & Azaisbraesco, 1995;Parker, 1997;Ribeiro et al, 2006), which may enhance their health promoting activities.…”