Cellular viscoelasticity probed by active rheology in optical tweezers

Lyubin, Evgeny V.; Khokhlova, Maria D.; Skryabina, Mariya; Fedyanin, Andrey A.

doi:10.1117/1.jbo.17.10.101510

Cited by 29 publications

(11 citation statements)

References 17 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, the dualtrap optical tweezers were used in the micro-rheology applications with the two beams trapping the RBC at its opposite edges, with or without attaching microbeads. With one of the beams oscillating at a small amplitude and a frequency up to 1 KHz to introduce fluctuation, the phase signal of the cell edge displacement vibration was recorded as a function of the oscillation frequency to probe the RBC viscoelastic properties [7,8].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional light-scattering and deformation of individual biconcave human blood cells in optical tweezers

Yu¹,

Sheng²,

Chiou³

2013

Opt. Express

View full text Add to dashboard Cite

For studying the elastic properties of a biconcave red blood cell using the dual-trap optical tweezers without attaching microbeads to the cell, we implemented a three-dimensional finite element simulation of the light scattering and cell's deformation using the coupled electromagnetic and continuum mechanics modules. We built the vector field of the trapping beams, the cell structure layout, the hyperelastic and viscoelastic cell materials, and we reinforced the constraints on the cell constant volume in the simulation. This computation model can be useful for studying the scattering and the other mechanical properties of the biological cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Three-dimensional light-scattering and deformation of individual biconcave human blood cells in optical tweezers

Yu¹,

Sheng²,

Chiou³

2013

Opt. Express

View full text Add to dashboard Cite

show abstract

“…on two different fibroblast lines . Laser‐induced erythrocyte edge vibrations were employed for probing the viscoelasticity of red blood cells . Ayala et al.…”

Section: Applications For Biological Samplesmentioning

confidence: 99%

“…[163] Laser-induced erythrocyte edge vibrations were employed for probing the viscoelasticity of red blood cells. [164] Ayala et al employed optical tweezers to determine the microrheological properties of fibroblasts, astrocytes, and neurons. [165] Nishizawa et al recently reported a feedback-tracking method for microrheology in mouse fibroblasts and HeLa cells.…”

Section: Microrheology Measurementsmentioning

confidence: 99%

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Bunea

Glückstad

2019

Laser & Photonics Reviews

View full text Add to dashboard Cite

Optical trapping and manipulation of objects down to the Ångstrom level has revolutionized research at the smallest scales in all natural sciences. The flexibility of optical trapping methods facilitates real‐time monitoring of the dynamics of biological processes in model systems and even in living cells. Different optical trapping and manipulation approaches allow displacement of nanostructures with subnanometer precision and force measurements with femtonewton precision. Due to inherent constraints of optical methods, most optical trapping experiments are performed in water or simple aqueous solutions. However, in recent years, there is an ever‐growing interest of shifting from simple aqueous media towards more biologically‐relevant media. Precise optical trapping and manipulation, combined with state‐of‐the‐art microfabrication, will enable the development of microrobotic “surgeons” with tremendous potential for biomedical and microengineering applications. This review introduces the basics of optical trapping and discusses its applications for biological samples, with focus on trapping in biological media and strategies for overcoming the challenges of optical manipulation in complex environments as a stepping‐stone for microrobotic “surgeons.”

show abstract

“…The laser beam can also trap the proximity of the cell or vibrate in its position for an active rheology assay. 1,2,5 In a recent paper, we have analyzed the local stress, local strain, and the deformation of a 1-D viscoelastic solid rod and of a 3-D biological cell subjected to an external jumping load at a high frequency using the finite-element method (FEM). 1 In many cases, however, the analysis on the mechanics of the laser beam manipulation of the biological cell was approximate.…”

Section: Introductionmentioning

confidence: 99%

Temporal response of three-dimensional biological cells to high-frequency optical jumping tweezers

Sheng

2015

J. Nanophoton

View full text Add to dashboard Cite

We analyzed the temporal responses of biological cells in the jumping optical tweezers for tugging, wiggling, and stretching the cells in the time-sharing regime with the finiteelement method. We showed that the jumping of local stress and local strain is independently omnipresent on the recovery time of the viscoelastic material and the jumping frequency of the load. We demonstrated that the elongation of a three-dimensional (3-D) viscoelastic object under a jumping load cannot be evaluated using the one-dimensional spring-dashpot material model without considering its 3-D structure. Temporal response of three-dimensional biological cells to high-frequency.

show abstract

Cellular viscoelasticity probed by active rheology in optical tweezers

Cited by 29 publications

References 17 publications

Three-dimensional light-scattering and deformation of individual biconcave human blood cells in optical tweezers

Three-dimensional light-scattering and deformation of individual biconcave human blood cells in optical tweezers

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Temporal response of three-dimensional biological cells to high-frequency optical jumping tweezers

Contact Info

Product

Resources

About