Cellularity and Colony-Forming Units of Hematopoietic Tissue of Mice After Antilymphocytic Serum Treatment

Gd, Ledney

doi:10.1097/00007890-197206000-00002

Cited by 4 publications

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“…Ledney et al have found that an ATS pretreatment of the donor mice for more than 4 consecutive days abrogates the GVHD inducing capacity of the spleen cells in irradiated semiallogeneic recipients [26]. In a syngeneic model, after another protocol of donor ATS treatment, they demonstrated an increased frequency of splenic colony forming units [27].…”

Section: Discussionmentioning

confidence: 99%

Spleen Cells from Antithymocyte Serum Pretreated Mice Do Not Induce GVHD but Exert Increased Repopulating Activity in Irradiated Semiallogeneic Recipients

1995

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Graft-versus-host disease (GVHD) due to allogeneic bone marrow transplantation can be prevented by depleting the T cells from the marrow graft in vitro. However, the elimination of the donor T cells results in a higher frequency of graft failure, secondary infections and, in case of leukaemia, relapse. We found, that, in contrast to normal spleen cells, spleen cells from A or B10 donor mice pretreated with xenogeneic antithymocyte serum (ATS) in vivo did not induce GVHD in non-irradiated (B10 x A)F1 hybrids. Spleen cells of ATS-pretreated A donors did not cause GVHD in allogeneic CBA mice made neonatally tolerant to the A donor strain either. Furthermore, spleen cells from ATS-treated donors did not cause GVHD in irradiated F1 hybrid recipients, moreover, they decreased the lethal effect of irradiation. The in vivo ATS pretreatment improved the repopulating capacity of spleen cells in irradiated syngeneic recipients, too. The effect of the ATS treatment does not rely solely upon the elimination of T cells, since flow cytofluorometric analysis revealed only a partial depletion of both the CD4+ and CD8+ T cells of the ATS-pretreated animals. These observations may also have clinical relevance.

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Section: Discussionmentioning

confidence: 99%

Spleen Cells from Antithymocyte Serum Pretreated Mice Do Not Induce GVHD but Exert Increased Repopulating Activity in Irradiated Semiallogeneic Recipients

1995

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“…Anti-lymphocyte sera may be used in order to suppress the immune responses of patients with transplants (Bach, 1970). Animal experiments have indicated that anti-lymphocyte sera might also lead to an adverse reaction, with suppression of the haematopoietic capacity (DeMeester et a!, 1968; Field & Gibbs, 1968;Floersheim & Ruszkiewicz, 1969;Gengozian & Congdon, 1969;Seller & Polani, 1969;Cole & Maki, 1971 ;Chertkov et al, 1972;Gallagher et al, 1972;Ledney, 1972 Haematopoietic progenitor cells might theoretically carry lymphocyte specific antigens since mature, immune competent lymphocytes originate from such progenitors. However, as the ultimate progenitor cells are believed to be pluripotent (Metcalf & Moore, 1971) the cell-line specific antigens must probably be acquired, or at least accumulated, during the differentiation and maturation processes (RaK 1969;Chan et al, 1970;Raff et al, 1971;Takahashi et a!, 1971 ;El-Arini & Osoba, 1973).…”

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confidence: 99%

Effect of Heterologous Anti‐lymphocyte and Anti‐granulocyte Sera on Haematopoietic Progenitor Cells in the Rat

1974

Br J Haematol

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The effect on haematopoietic progenitor cells of specific antisera against mature haemic cells has been investigated.Heterologous anti-lymphocyte and anti-granulocyte sera were prepared by injecting suspensions of rat target cells into rabbits. Recirculating lymphocytes were obtained from thoracic duct lymph, non-recirculating ones from blood of rats in Experimental Medicine, 134, 815. TRENTIN, J.J. & JUDD, K.P. (1973) Prevention of acute graft-versus-host (GVH) mortality with spleenabsorbed antithymocyte globulin (ATG). Transplantation Proceedings, 5, 865.

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Graft-versus-Host-Reactions: A Review

1976

Advances in Immunology

189

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Cellularity and Colony-Forming Units of Hematopoietic Tissue of Mice After Antilymphocytic Serum Treatment

Cited by 4 publications

References 0 publications

Spleen Cells from Antithymocyte Serum Pretreated Mice Do Not Induce GVHD but Exert Increased Repopulating Activity in Irradiated Semiallogeneic Recipients

Spleen Cells from Antithymocyte Serum Pretreated Mice Do Not Induce GVHD but Exert Increased Repopulating Activity in Irradiated Semiallogeneic Recipients

Effect of Heterologous Anti‐lymphocyte and Anti‐granulocyte Sera on Haematopoietic Progenitor Cells in the Rat

Graft-versus-Host-Reactions: A Review

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