Cellulase Adsorption on Pretreated Sugarcane Bagasse During Enzymatic Hydrolysis

Neto, João Moreira; Machado, Daniele Longo; Bonomi, Antonio; Gonçalves, Vinicius Ottonio O.; Martins, Luiza Helena da Silva; Costa, Josiel Martins; Costa, Aline Carvalho da

doi:10.1007/s12355-023-01302-y

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“…where a, b, and c are constants and X is the conversion (%). Values of a, b, and c are displayed in Table 5, as adjusted in a previous study [35].…”

Section: Kinetic Modelmentioning

confidence: 99%

A Novel Kinetic Modeling of Enzymatic Hydrolysis of Sugarcane Bagasse Pretreated by Hydrothermal and Organosolv Processes

et al. 2023

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Lignocellulosic biomasses have a complex and compact structure, requiring physical and/or chemical pretreatments to produce glucose before hydrolysis. Mathematical modeling of enzymatic hydrolysis highlights the interactions between cellulases and cellulose, evaluating the factors contributing to reactor scale-up and conversion rates. Furthermore, this study evaluated the influence of two pretreatments (hydrothermal and organosolv) on the kinetics of enzymatic hydrolysis of sugarcane bagasse. The kinetic parameters of the model were estimated using the Pikaia genetic algorithm with data from the experimental profiles of cellulose, cellobiose, glucose, and xylose. The model considered the phenomenon of non-productive adsorption of cellulase on lignin and inhibition of cellulase by xylose. Moreover, it included the behavior of cellulase adsorption on the substrate throughout hydrolysis and kinetic equations for obtaining xylose from xylanase-catalyzed hydrolysis of xylan. The model for both pretreatments was experimentally validated with bagasse concentration at 10% w/v. The Plackett–Burman design identified 17 kinetic parameters as significant in the behavior of process variables. In this way, the modeling and parameter estimation methodology obtained a good fit from the experimental data and a more comprehensive model.

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“…where a, b, and c are constants and X is the conversion (%). Values of a, b, and c are displayed in Table 5, as adjusted in a previous study [35].…”

Section: Kinetic Modelmentioning

confidence: 99%