Cellulase and Xylanase Production by Yeasts of the Genus Trichosporon

Stevens, Barry J.H.; Payne, J. M.

doi:10.1099/00221287-100-2-381

Cited by 47 publications

(17 citation statements)

References 27 publications

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“…Commercial larchwood xylan was purified using the procedure described by Stevens and Payne (1977) as well as the method described by J. P. Utille (1979. Thesis, University of Grenoble).…”

Section: Chemicalsmentioning

confidence: 99%

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Production, purification, and properties of thermostable xylanase from Clostridium stercorarium

Bérenger¹,

Frixon²,

Bigliardi³

et al. 1985

Can. J. Microbiol.

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The production of xylanase and endoglucanase in Clostridium stercorarium has been studied in different conditions. Activities were higher when the organism was grown on xylan and cellulose than on soluble substrates. Catabolite repression of xylanase synthesis occurred when glucose and other readily metabolizable substrates were added during growth on cellulose. Three endoxylanases, A, B, and C, from culture filtrate were purified to homogeneity. Most of the properties of xylanases A, B, and C were similar (optimum pH in the range of 5.5–7.0 at 65 °C; isoelectric pH, 4.4–4.5; Km values of 2.9–3.7 mg/mL). The enzymes were inactivated by Hg2+ and p-chloromercuribenzoate but slight inhibition was obtained with more specific thiol reagents such as 5,5′-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide. Carbohydrate content (3–19%) and half-life (2 min 30 s to 90 min) varied. Patterns of hydrolysis demonstrate that the three enzymes are endo-splitting enzymes able to break down xylan at random giving xylobiose and xylotriose as the main end products. The three enzymes exhibited immunological cross-reactivity.

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“…Commercial larchwood xylan was purified using the procedure described by Stevens and Payne (1977) as well as the method described by J. P. Utille (1979. Thesis, University of Grenoble).…”

Section: Chemicalsmentioning

confidence: 99%

“…Effects of substrates on xylanase and endoglucanase levels in Clostridium stercorarium *Purified by method 1 as reported byStevens and Payne (1977). thrified by method 2 as reported by J .…”

mentioning

confidence: 99%

Production, purification, and properties of thermostable xylanase from Clostridium stercorarium

Bérenger¹,

Frixon²,

Bigliardi³

et al. 1985

Can. J. Microbiol.

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“…As can be seen, the main sugars are D-glucose and D-mannose whereas D-xylose represents a relatively low percentage. Purification of xylan was attempted by the method of Aspinall and McKay (1958), as modified by Stevens and Payne (1977), but with unsatisfactory results. Better results were obtained by treating the commercial polysaccharide with zymolyase 5000 (48 h at 37"C, pH 7.5) in a proportion of 5: 1 (w/w).…”

Section: Xylan Purijicationmentioning

confidence: 99%

β-D-Xylanases of Bacillus circulans WL-12

Esteban¹,

Villanueva²,

Villa³

1982

Can. J. Microbiol.

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Bacillus circulans WL-12 secretes two endo-β-D-xylanases (A and B, respectively) (EC 3.2.1.8.) and one β-D-xylosidase (EC 3.2.1.37) when cultured in liquid media with xylan as the sole carbon source. Xylanases A and B have been partially characterized with respect to their main physicochemical parameters and β-D-xylosidase to a lesser extent on account of its low stability. Both endo-β-D-xylanase A and β-D-xylosidase were adsorbed on DEAE-Biogel A, had similar molecular weights (approximately 85 000), and had optimum pH values of 5.5–7, but exhibited different isoelectric points (4.5 for β-D-xylanase A and 4.7 for β-D-xylosidase) and different mobilities in polyacrylamide gel electrophoresis. The apparent Michaelis constant for β-D-xylanase A was 8 mg∙mL−1 and the hydrolysis products produced were xylose, xylobiose, xylotriose, and xylotetraose.The second endo-β-D-xylanase (β-D-xylanase B) bound to CM-Biogel A and exhibited a molecular weight of approximately 15 000 and an optimum pH value in the range of 5.5–7. The isoelectric point was 9.1 and the apparent Michaelis constant was 4 mg∙mL−1. The hydrolysis products produced by this enzyme were xylobiose, xylotriose, and xylotetraose, but never xylose. In polyacrylamide gel electrophoresis at pH 8 the enzyme moved towards the negative electrode.

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“…Os fungos são os principais microrganismos utilizados pela indústria na produção de enzimas (MENEZES, 1997) PARK, 1994). Algumas leveduras como as do gênero Trichosporium sp também são produtoras de xilanases e celulases, assim como diversas espécies de Aspergillus produzem altos níveis de -glicosidase (STEVENS;PAYNE, 1977).…”

Section: Hidrólise Enzimáticaunclassified

Panorama e perspectivas do etanol lignocelulósico

Silva¹

2012

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A necessidade de um novo padrão energético e a configuração de um arranjo produtivo baseado no uso de biomassa vêm impulsionando uma série de pesquisas e inovações. A possibilidade de produzir etanol de resíduos lignocelulósicos é um grande atrativo para os pesquisadores, pois dificuldades, como a necessidade de aumentar a área plantada para incremento da produção e alto custo de matéria-prima, deixam de existir. Diante disso, o presente trabalho foi guiado por uma pesquisa exploratória cujas ideias e hipóteses apontaram os caminhos atuais mais promissores para a produção de etanol, a partir de lignocelulose. O estudo mostrou que o Brasil tem investido muito em pesquisas para aprimorar as técnicas de produção, mas muitos estudos ainda precisam ser feitos em cada etapa do processo produtivo, para tornar o bioetanol lignocelulósico viável economicamente.

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Cellulase and Xylanase Production by Yeasts of the Genus Trichosporon

Cited by 47 publications

References 27 publications

Production, purification, and properties of thermostable xylanase from Clostridium stercorarium

Production, purification, and properties of thermostable xylanase from Clostridium stercorarium

β-D-Xylanases of Bacillus circulans WL-12

Panorama e perspectivas do etanol lignocelulósico

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