Cellulase end xylanase activity of fungi in a collection isolated from the southern Caucasus

Kvesitadze, E.; Adeishvili, E.; Gomarteli, M.; Kvachadze, L. L.; Kvesitadze, G.

doi:10.1016/s0964-8305(99)00053-0

Cited by 12 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…is widely present on plant material and can cause post-harvest spoilage of fruit [34][35][36][37][38], even at a low temperature. D. dimorphosporum is industrially used as a producer of plant cell wall lytic enzymes [39,40]. R. vagum, infecting roots and tubers, is known to contribute to vine decline and root necrosis [41][42][43].…”

Section: Diversity Of Yeasts and Molds And Modulation During Storagementioning

confidence: 99%

Changes of Quality of Minimally-Processed Pineapple (Ananas comosus, var. ‘Queen Victoria’) during Cold Storage: Fungi in the Leading Role

et al. 2020

View full text Add to dashboard Cite

Minimally-processed pineapple stored under refrigerated conditions is highly perishable. We aimed to characterize the evolution of physicochemical, sensory and microbiological quality during cold storage. Pineapple batches were sampled from several locations in Reunion Island and then minimally processed. In the processing step, the variability of firmness and counts of yeasts and molds were observed. Moreover, correlations between the sampling season and pH and b* color component, as well as between fungal population and b* parameter were observed. During storage, the visual aspect of pineapple cuts changed to brown and shiny, whereas olfactive descriptors shifted from fruity descriptors and fresh to fermented, alcoholic and milky. The values for pH, TA and TSS did not significantly vary according to storage time. A decrease in firmness and C* color parameter was observed. Yeast and mold counts were significantly higher after 7 days of storage. The diversity in yeasts and molds was mainly dependent on the considered batches observed from PCR-DGGE profiles. Fungal species were isolated from spoiled pineapple cuts. The implication of Penicilllium citrtrinum, Talaromyces amestolkiae, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, and Meyerozyma caribbica in the spoilage of minimally-processed pineapple cuts was further demonstrated.

show abstract

Section: Diversity Of Yeasts and Molds And Modulation During Storagementioning

confidence: 99%

Changes of Quality of Minimally-Processed Pineapple (Ananas comosus, var. ‘Queen Victoria’) during Cold Storage: Fungi in the Leading Role

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The highest amylase activity was associated with A. flavipes, T. harzianum, F. niveus, and A. niger. The production of amylases, pectinases and cellulases by fungi was reported by many workers (Joshi, et al, 1993;Abdel-Sater, 1994;Cavalitto, et al, 1996;Ugwaunyi and Obeta, 1997;Kvesitadze, et al, 1999;Celestino, et al, 2005;and Jorgensen and Olsson, 2006).…”

Section: Enzymatic Activitymentioning

confidence: 78%

Studies on Fungal Communities Associated with Litter of Plant Cover at Al-Taif Province, Saudi Arabia

Kabli¹

2007

met

View full text Add to dashboard Cite

Twenty one fungal species belonging to ten genera were isolated from the litter of eight dominant plants cover of Al-Taif province, Saudi Arabia. The most frequently isolated fungal species were Fusarium oxysporum (100%), Aspergillus alutaceus, A. niger, and Macrophomina phaseolina (87.5%), F. solani, Mucor racemosus, Penicillium glaburum (75%), and P. jaczewskii (62.5%). The influence of temperature and pH on selected eleven isolates, representing the dominant fungal species, was estimated. The representative moulds failed to grow at 55ºC temperature. A flavipes, A. niger and Emericella nidulans showed thermotolerant activity (optimum growth at 35ºC). The selected fungal species responded differently to the tested pH values of Czapek-Dox medium; some appeared to be acidophilic (P. jaczewskii, T. harzianum, F. niveus, A. flavipes and A. melleus, pH 3.5-4.5), some alkaliphilic (Emericella nidulans and Gliocladium roseum, pH 8.1) and a third group attained their best growth values around neutral pH (5.9-6.8). The selected moulds were capable of producing cellulytic, pectinolytic and amylolytic enzymes, which indicate their major role in litter decomposition.

show abstract

“…These results support the importance of performing large screening programs in order to identify potential cellulase producer wild-types which may present a better enzymatic profile than T. reesei Rut-C30, given a specific growth substrate. Although large collections of Trichoderma strains have already been screened in terms of cellulase activity, little information on the distribution of the trait has been provided up to now (Kovacs et al, 2008;Kvesitadze et al, 1999). The estimation of the trait dispersion around the population mean gives valuable indications for the sample size definition in a screening program (Fig.…”

Section: Discussionmentioning

confidence: 99%

A novel microplate‐based screening strategy to assess the cellulolytic potential of Trichoderma strains

Cianchetta

Galletti

Burzi

et al. 2010

Biotech & Bioengineering

View full text Add to dashboard Cite

Bioconversion of lignocellulosic biomass to fuel requires a hydrolysis step to obtain fermentable sugars, generally accomplished by fungal enzymes. An assorted library of cellulolytic microbial strains should facilitate the development of optimal enzyme cocktails specific for locally available feedstocks. Only a limited number of strains can be simultaneously assayed in screening based on large volume cultivation methods, as in shake flasks. This study describes a miniaturization strategy aimed at allowing parallel assessment of large numbers of fungal strains. Trichoderma strains were cultivated stationary on microcrystalline cellulose using flat bottom 24-well plates containing an agarized medium. Supernatants obtained by a rapid centrifugation step of the whole culture plates were evaluated for extracellular total cellulase activity, measured as filter paper activity, using a microplate-based assay. The results obtained were consistent with those observed in shake-flask experiments and more than 300 Trichoderma strains were accordingly characterized for cellulase production. Five strains, displaying on shake-flasks at least 80% of the activity shown by the hyper-cellulolytic mutant Trichoderma Rut-C30, were correctly recognized by the screening on 24-well plates, demonstrating the feasibility of this approach. Cellulase activity distribution for the entire Trichoderma collection is also reported. One strain (T. harzianum Ba8/86) displayed the closest profile to the reference strain Rut-C30 in time course experiments. The method is scalable and addresses a major bottleneck in screening programs, allowing small-scale parallel cultivation and rapid supernatant extraction. It can also be easily integrated with high-throughput enzyme assays and could be suitable for automation.

show abstract

Cellulase end xylanase activity of fungi in a collection isolated from the southern Caucasus

Cited by 12 publications

References 14 publications

Changes of Quality of Minimally-Processed Pineapple (Ananas comosus, var. ‘Queen Victoria’) during Cold Storage: Fungi in the Leading Role

Changes of Quality of Minimally-Processed Pineapple (Ananas comosus, var. ‘Queen Victoria’) during Cold Storage: Fungi in the Leading Role

Studies on Fungal Communities Associated with Litter of Plant Cover at Al-Taif Province, Saudi Arabia

A novel microplate‐based screening strategy to assess the cellulolytic potential of Trichoderma strains

Contact Info

Product

Resources

About