Cellulase in Anoplophora glabripennis adults fed on original host tree species and non-original host trees

Abstract

Cellulase activities of Anoplophora glabripennis (Motsch.) adults from two host plants (Populus simonii × P. pyramidliscr cv. Opera Hsu. and Salix matsudana Koidz) fed on three different host tree species (Acer negundo Linn., S. matsudana Koidz and P. simonii × P. pyramidliscr cv. Opera Hsu.) were investigated. Enzyme activities of endoglucanase and β-glucosidase in the intestines of the insects were measured. The results show that there are no statistically significant differences in the enzyme activities of endoglucanase between male and female insects from the two host plants fed on three host trees, neither in β-glucosidase. No statistically significant differences in the enzyme activities of endoglucanase and β-glucosidase were found between males and females from the two plants fed on three host tree species, except that the endoglucanase activity in males from the opera poplar fed on Hankow willow is lower than that fed on ash-leaf maple and opera poplar.

Gut Lignocellulose Activity and Microbiota in Asian Longhorned Beetle and Their Predicted Contribution to Larval Nutrition

“…pyramidalis , characterized by strong resistance, lignocellulose enzyme activity was lowest ( Figure 2 ). This result is consistent with previous studies by Geib et al (2010) and Li et al (2010) . In addition, Chen et al (2006) concluded that the order of cellulase activities in the guts of A. glabripennis larvae was β-1,4-glucosidase > β-1,4-endoglucanase > β-1,4-exoglucanase, consistent with our current results.…”

“…For β-exoglucanase activity, 200 μL of a 1% avicel solution was combined with 100 μL of crude gut extract ( Kukor et al, 1988 ). For β-glucosidase activity, 200 μL of a 1% salicin solution was combined with 100 μL of diluted enzyme solution (crude gut extract diluted five times with acetic acid-sodium acetate buffer) ( Li et al, 2010 ). For β-endoglucanase activity, 200 μL of 1% carboxymethylcellulose solution was combined with 100 μL of diluted enzyme solution ( Kukor et al, 1988 ).…”