Cellulase production and oil palm empty fruit bunch saccharification by a new isolate of Trichoderma koningii D-64

Wang, Zunsheng; Ong, Hui Xin; Geng, Anli

doi:10.1016/j.procbio.2012.07.001

Cited by 30 publications

(20 citation statements)

References 37 publications

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“…Lignocellulose is one of the renewable resources and abundant biopolymers in the earth [3]. In Indonesia, oil palm empty fruit bunch was considered as a potential feedstock that could be used for bioethanol production.…”

Section: Introductionmentioning

confidence: 99%

Comparison of SHF and SSF Processes Using Enzyme and Dry Yeast for Optimization of Bioethanol Production from Empty Fruit Bunch

et al. 2015

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Empty fruit bunch was considered as substrate for second generation of bioethanol because it consists of lignin, cellulose, and hemicelluloses. For lignocelluloses materials, it usually needs pretreatment and hydrolysis to convert cellulose into glucose. Two methods of enzymatic hydrolysis, Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) were carried out in this study. The performance of both SHF and SSF was concerned to evaluate the effect of hydrolysis methods and enzyme concentration for producing ethanol. Pretreatment was conducted in a reactor using 10% sodium hydroxide at temperature 150°C during 30 minutes. Two kinds of enzyme, Cellic® CTec2 and Cellic® HTec2 from novozyme were added in 15% (gr/ml) of pretreated EFB at pH 4.8. Four concentration of enzyme Cellic® CTec2, 10, 20, 30, 40 FPU per gram biomass were performed in SHF and SSF processes respectively, while Cellic® HTec2 was added 20% from Cellic® CTec2. Contents of glucose, xylose, and ethanol were recorded every 24 hours. Using 40 FPU of concentration enzyme, it could be produce 4.74% of ethanol in 72 hours fermentation by SHF process and 6.05% of ethanol in 24 hours by SSF process. From this study, the SSF method was considered as a better process than SHF due to rapidly ethanol production and the highest concentration of produced ethanol.

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Section: Introductionmentioning

confidence: 99%

Comparison of SHF and SSF Processes Using Enzyme and Dry Yeast for Optimization of Bioethanol Production from Empty Fruit Bunch

et al. 2015

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“…The cost of cellulolytic enzymes is a major factor in the hydrolysis of lignocellulosic materials to fermentable sugars [47]. Currently, various cellulolytic microorganisms were from fungi, such as Aspergillus [48,49], Penicillium [50,51], Trichoderma [30,[52][53][54], and Talaromyces [55][56][57], which required long production cycles. Compared with fungi, bacteria not only have a higher growth rate, but also produce cellulase in a short time.…”

Section: Cellulolytic Activity Of Cellulolytic Marine Bacterial Strainsmentioning

confidence: 99%

Isolation and Characterization of Cellulolytic Marine Bacteria for Litopenaeus Vannamei Aquaculture Using Sugarcane Bagasse as Carbon Source

Wei¹,

Xu²,

Long³

et al. 2020

Preprint

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Background: In aquaculture system, it is essential to adjust the inherent disadvantage of C/N ratio by adding a lot of additional carbon sources, such as sugarcane molasses, organic acids, and organic acid salts, which will greatly increase the cost of shrimp aquaculture. Herein, we aimed to isolate cellulolytic marine bacteria to hydrolyze sugarcane bagasse (SB) for reducing the cost of addition of external carbon sources in industrial Litopenaeus vannamei aquaculture.Results: A total of 97 cellulolytic marine bacterial strains belonged to 6 genera were isolated from 2,585 indigenous bacteria, indicating that seagrass bed can be used as an important place for screening the cellulolytic bacteria. The hydrolysis capacity (HC) of 58 cellulolytic marine bacterial strains was ranged from 1.1–4.0. MW-M5 displayed the largest HC value, followed by MW-M10 and MW-M14. The cellulase contents of 30 strains were more than 3 U/g in the supernatant of fermentation broth after 24 h, which was significantly higher than that of commercial cellulose. 26 cellulolytic marine bacteria with HC greater than 2 were safe for L. vannamei. MW-M19 with the lowest multiple antibiotic resistance index, 0.1, had a highest SB enzyme activity, 4.14 U/mL. The SB decomposition rates of CFW-C18 and MW-M15 were up to about 63.81% and 48.57% after fifteen days, respectively. Conclusions: These results provide valuable information for further construction of a shrimp aquaculture system based on low-cost external carbon sources using cellulolytic bacteria, and even for other biotechnological applications.

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“…This contributes to a decrease in biomass accessibility towards the hydrolytic enzymes, which then results in higher rate of non-specific adsorption of the enzymes and further causes decline in enzyme activities 46 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 glucose and cellobiose as the end-products of hydrolysis process. However, high saturation of these end-products inactivates hydrolytic enzymes, especially cellulases, by blocking their active sites which causes less depolymerisation of cellulose at higher substrate concentration 47 .…”

Section: Influences Of Substrate Loading On Opt Hydrolysis Using Crudmentioning

confidence: 99%

Potential Uses of Xylanase-Rich Lignocellulolytic Enzymes Cocktail for Oil Palm Trunk (OPT) Degradation and Lignocellulosic Ethanol Production

et al. 2015

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In this paper, we reported that oil pam trunk (OPT) can be used as an alternative fermentation feedstock for lignocellulolytic enzyme production and carbon source for bioethanol production. Xylanase production from OPT by locally isolated fungus, Aspergillus fumigatus SK1, under solid state fermentation (SSF) was optimized using central composite design (CCD). Under optimized conditions, a maximum xylanase activity of 1792.43 U/g was produced, which was 4.28 folds higher than before optimization. Significant amount of CMCase (56.19 U/g), FPase (3.47 U/g), and β-glucosidase (1.55 U/g) were also found concomitantly with xylanase. Subsequently, the effect of solid loading, Tween-80 concentration and incubation temperature on saccharification of OPT by the crude enzymes were optimized to enhance the total reducing sugar production. A total of 13.148 g/L of reducing sugar was reported at optimized condition. The comparisons of physiochemical characteristics between native and hydrolysed OPT by SEM, FTIR and XPS showed strong degradation capacity of the crude enzymes towards cellulose, hemicellulose and lignin.Alcoholic fermentation of the hydrolysate by Candida tropicalis RETL-Crl produced 3.067 g/L of ethanol. Higher ethanol production at 0.322 g/g with theoretical ethanol yield of 68.05%indicates Candida tropicalis RETL-Crl has a greater potential to be used in ethanol fermentation process. This result further proved that OPT has potential to be used as a renewable carbon source.

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Cellulase production and oil palm empty fruit bunch saccharification by a new isolate of Trichoderma koningii D-64

Cited by 30 publications

References 37 publications

Comparison of SHF and SSF Processes Using Enzyme and Dry Yeast for Optimization of Bioethanol Production from Empty Fruit Bunch

Comparison of SHF and SSF Processes Using Enzyme and Dry Yeast for Optimization of Bioethanol Production from Empty Fruit Bunch

Isolation and Characterization of Cellulolytic Marine Bacteria for Litopenaeus Vannamei Aquaculture Using Sugarcane Bagasse as Carbon Source

Potential Uses of Xylanase-Rich Lignocellulolytic Enzymes Cocktail for Oil Palm Trunk (OPT) Degradation and Lignocellulosic Ethanol Production

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