Cellulase production in Lysinibacillus sp isolated from the estuaries of Odisha

Mahalik, Shubhashree; Mohapatra, Deepali; Kumar, Dhanesh

doi:10.21786/bbrc/11.4/27

Cited by 6 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They gave negative results in this experiment. These results were disagree with Mahalik et al, (2018) studied that the secondary metabolites, enzymes, and proteins are abundant in microorganisms. Cellulase is a key component of the enzyme cocktail utilised for the breakdown of lignocellulosic biomass, and the capacity to produce cellulase has been identified in Lysinibacillus sp., and in contrast to Khianngam et al, (2014), Based on the decolorization of CMC-basal agar media with Congo red as a colour identifier, the bacteria were grown to have the ability to degrade carboxymethyl-cellulose (CMC).…”

Section: Cellulase Enzyme Detectioncontrasting

confidence: 84%

Biological Control for some Insects by Using Plant Growth Promoting Bacteria in Laboratory and Field Conditions

Ali

Ibrahim

2023

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

The cotton leafworm and aphid insects are major pests in Egypt that attack many host plants. Pesticides use can cause different problems in the plant system. So, it is very important to use nature products to be safe as a alternatives to synthetic pesticides. This research aimed to evaluate the efficacy of three bacterial types: Lysinibacillus macroides, Brevundimonas olei and Acinetobacter sp. in laboratory and field conditions throughout two seasons 2021 & 2022. L. macroides and B. olei showed more toxicity and LC50 levels than Acinetobacter sp with > 90 % corrected mortality towards the cotton leafworm and bean aphid after 3 days exposure time under laboratory conditions. Under field conditions, there were two insect pests fall armyworm (Spodoptera frugiperda) and aphid insects (Myzus persica (Sulzer) that investigate pepper plants in Ismallia Governorate. The results also showed a high efficacy of two bacterial strains, L. macroides and B. olei against M. persicae and S. frugiperda with more than 80% and 60% reduction percent respectively. The data stated that, L. macrolides strain was the most effective on suppressing the aphid and fall armyworm insects population. Some bacterial enzymes and biochemical products produced by bacteria used, such as protease, chitinase, catalase, glutathione peroxidase, polyphenol oxidase (PPOs) and HCN. They play a role in insect population reduction in laboratory and field conditions throughout integrated crop management.

show abstract

Section: Cellulase Enzyme Detectioncontrasting

confidence: 84%

Biological Control for some Insects by Using Plant Growth Promoting Bacteria in Laboratory and Field Conditions

Ali

Ibrahim

2023

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

show abstract

“…All identified bacteria are known to be potentially cellulolytic (Awasthi et al, 2015 ). Mahalik et al ( 2018 ) used the strain of Lysinibacillus sp. in submerged fermentation to produce cellulases.…”

Section: Resultsmentioning

confidence: 99%

Influence of the autochthonous cellulolytic bacteria on the domestic compost process improvement

Ćwiertniewicz-Wojciechowska¹,

Ślipko²,

Banach-Wiśniewska³

et al. 2023

bta

View full text Add to dashboard Cite

This work aimed to determine the influence of the inoculation of autochthonous cellulolytic bacteria on the composting process without any modifications of physical or chemical parameters. Bacteria with cellulolytic abilities were isolated from composted material containing food and plant leftovers and identified as Bacillus licheniformis, Bacillus altitudinis, and Lysinibacillus xylanilyticus. The experimental composter containing garden and household wastes was inoculated with bio-vaccine prepared as a mixture of isolated cellulolytic bacterial strains and composted for the next 96 days parallelly to the control composter without the inoculation. During the experiment, changes in temperature, humidity, the content of the humic acids (HAs), organic carbon, nitrogen, and C:N ratio were determined. As the particular microbial groups play a key role in the composting process, the biodiversity of the microorganisms present in the composter as well as the number of psychrophilic, mesophilic, and sporeforming microorganisms, Actinomycetes, and fungi were analyzed. The changes in the abundance of particular bacterial groups were convergent with temperature changes in the temperature of composting material. The composting material inoculated with autochthonous microorganisms was characterized by higher HA content and lower biodiversity. The inoculation with autochthonous microorganisms positively influenced the composting material in the corners for the entire process and in the middle of the container for 61 days. Thus, the effect of inoculation depended on the localization of the process inside the container subjected to biopreparation.

show abstract

“…DLP method based on plasmid pSHY211 was established, which can quickly screen active clones. In particular, many functional enzymes related to the degradation of macromolecular substrates, such as proteins, and polysaccharides need to be secreted outside the cell 25 , 26 . To establish rapid screening of clones on the plate, the signal peptide of functional enzymes must be recognized, transported extracellularly, and removed by E. coli 27 , 28 .…”

Section: Discussionmentioning

confidence: 99%

Characterization of a GH10 extremely thermophilic xylanase from the metagenome of hot spring for prebiotic production

Yin,

Li,

Long

et al. 2023

Sci Rep

View full text Add to dashboard Cite

A xylanase gene (named xyngmqa) was identified from the metagenomic data of the Gumingquan hot spring (92.5 °C, pH 9.2) in Tengchong City, Yunnan Province, southwest China. It showed the highest amino acid sequence identity (82.70%) to endo-1,4-beta-xylanase from Thermotoga caldifontis. A constitutive expression plasmid (denominated pSHY211) and double-layer plate (DLP) method were constructed for cloning, expression, and identification of the XynGMQA gene. The XynGMQA gene was synthesized and successfully expressed in Escherichia coli DH5α. XynGMQA exhibited optimal activity at 90 °C and pH 4.6, being thermostable by maintaining 100% of its activity after 2 h incubated at 80 °C. Interestingly, its enzyme activity was enhanced by high temperatures (70 and 80 °C) and low pH (3.0–6.0). About 150% enzyme activity was detected after incubation at 70 °C for 20 to 60 min or 80 °C for 10 to 40 min, and more than 140% enzyme activity after incubation at pH 3.0 to 6.0 for 12 h. Hydrolytic products of beechwood xylan with XynGMQA were xylooligosaccharides, including xylobiose (X2), xylotriose (X3), and xylotetraose (X4). These properties suggest that XynGMQA as an extremely thermophilic xylanase, may be exploited for biofuel and prebiotic production from lignocellulosic biomass.

show abstract

Cellulase production in Lysinibacillus sp isolated from the estuaries of Odisha

Cited by 6 publications

References 39 publications

Biological Control for some Insects by Using Plant Growth Promoting Bacteria in Laboratory and Field Conditions

Biological Control for some Insects by Using Plant Growth Promoting Bacteria in Laboratory and Field Conditions

Influence of the autochthonous cellulolytic bacteria on the domestic compost process improvement

Characterization of a GH10 extremely thermophilic xylanase from the metagenome of hot spring for prebiotic production

Contact Info

Product

Resources

About