Cellulase Production Under Solid-State Fermentation by Trichoderma reesei RUT C30: Statistical Optimization of Process Parameters

Mekala, Naveen Kumar; Singhania, Reeta Rani; Sukumaran, Rajeev K.; Pandey, Ashok

doi:10.1007/s12010-008-8156-9

Cited by 119 publications

(56 citation statements)

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“…High moisture encourages fungal growth, nutrient transportation and enzyme activities, but limits oxygen transfer and facilitates contamination [46,47]. A wide range of moisture contents from 50-89.5% have been used in various studies for cellulase production using A. niger [13].…”

Section: Optimization Of Moisture Content and Temperaturementioning

confidence: 99%

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Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined.Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cellulase production. Crude enzymes produced from MSW by T. reesei were evaluated for their ability to release glucose from MSW. A cellulose hydrolysis yield of 24.7% was achieved, which was close to that obtained using a commercial enzyme. Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes. Research has revealed that T. reesei can produce 13.4 FPU/g cellulase activity using water hyacinth [8]; 154.58 FPU/g using sugar cane bagasse [10]; 8.2 FPU/g using oil palm empty fruit bunches [11]; and 1.16 FPU/g using rice bran [12] as substrates. Similarly it has been reported that A. Niger could produce 24 FPU/g cellulase activity using wheat straw as a substrate [13]. Other substrates such as banana peel, rice straw, corn cob residue, rice husk, banana fruit stalk, and coconut coir pith have all being used for cellulase production [14,15]. Optimizing Cellulase Production fromAlthough MSW can contain a high lignocellulosic content, there has been limited research into the use of MSW for cellulase production. Other research demonstrated the possibility of using MSW to produce cellulase via SSF. But by using raw not autoclave MSW [16,17]. In this paper, we report an example of using an industrially processed MSW for cellulase production and then the use of the resulting "cellulase cocktail" for the enzymatic hydrolysis of MSW. Materials and Methods Municipal solid wasteMunicipal solid waste (MSW) was kindly supplied by Wilson Steam Storage Ltd (UK). The MSW had been subjected to commercial steam 15 min, respectively. After autoclaving, samples were neutralized to pH 7.0 using either 1 M NaOH or 1 M H 2 SO 4 as appropriate. The samples were then centrifuged at 5000 rpm (4472 g) for 10 min. The solid fraction (pellet) was rinsed three times by adding distilled water to the pellet and re-centrifuged. The solid fraction was then dried overnight at room temperature. Journal of Fundamentals of Renewable Energy and ApplicationsMicroorganisms: Two filamentous fungi, Trichoderma reesei QM6a and Aspergillus niger N402 were kindly donated by Professor David Archer (University of Nottingham, UK). Procedures for storing and cultivating T. reesei and A. n...

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Section: Optimization Of Moisture Content and Temperaturementioning

confidence: 99%

“…In addition to the preference of the microorganism, optimum moisture content may also be influenced by properties of the substrate such as porosity and particle size [46,48].…”

Section: Page 5 Of 10mentioning

confidence: 99%

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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“…There was a low production of CAEG at a higher range of moisture content, presumably due to the clumping of the medium resulting in low aeration and hyphal growth, which ultimately ended enzyme production. Likewise, a reduction in the solubility of substrate nutrients, a low degree of swelling, and high water tension were achieved due to low moisture content (Mekala et al, 2008).…”

Section: Moisture Contentmentioning

confidence: 99%

Optimization and characterization of cold-active endoglucanase produced by Aspergillus terreus strain AKM-F3 grown on sugarcane bagasse

Maharana¹,

Ray²

2015

Turk J Biol

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IntroductionPsychrotolerants are a more diverse group than true psychrophiles. The habitat of the latter is permanent cold regions, whereas the former can thrive in cold regions and also where mesophiles grow. The optimum and maximum growth temperatures for psychrotolerants are ≥15 °C and ≤40 °C, respectively (Morita and Moyer, 2001). Psychrotolerants can be found in deep oceans, Antarctica, the Arctic, high mountains, and man-made refrigerated systems (Russell and Cowan, 2006). There are few reports of psychrotolerant bacteria (Baghel et al., 2005;Maharana and Ray, 2013) and microfungi (Nazir et al., 2010;Maharana and Ray, 2014a) from the soil of India. The temperature of Jammu, India (32.73°N, 74.87°E) rarely reaches 37 °C and temperatures in the winter months occasionally fall below freezing.Extremozymes are seen to have immense future application in various fields of science. Enzymes from psychrotolerants are more adaptable than those from mesophiles and psychrophiles due to their wider range of temperature stability. Cold-active enzymes (CAEs) like lipase, cellulase, amylase, and protease produced from psychrotolerants are widely used in many industries, like the detergent, food, and textile industries; moreover, there is a high need for CAEs for biodegradation and bioremediation purposes in cold regions (Margesin and Schinner, 1999). Carboxymethyl cellulases (CMCase) or endoglucanases (EGs) (β-1,4-endoglucanase, EC 3.2.1.4) are inducible enzymes that are synthesized by microorganisms during their growth on cellulosic materials. In the textile industry, cold-active endoglucanases (CAEGs) can be used to remove unwanted extra fibrils from clothes so that the fabric is smoother and the clothes have increased longevity (Kumar et al., 2011). A higher temperature (60-70 °C) is needed for the saccharification of cellulose slurry and so more energy is consumed, which can be saved by the introduction of CAEGs to the slurry. Most reports of EGs were from fungi rather than bacteria and several researchers have reported on EGs produced from

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“…Several researchers have made an effort to reduce enzyme production costs or to enhance enzyme activity. Cheaper biomass substrates, cheaper saccharification techniques, and microbial strains with high enzyme productivities can be used to reduce costs (Mekala et al 2008). Additionally, media composition accounts for a considerable portion of cellulase production costs (Han et al 2009), and many studies have employed statistically designed experiments and response surface methodology (RSM) to optimize medium composition (Haltrich et al 1994;Singhania et al 2007;Han et al 2009;Valte et al 2010;Nagraj et al 2014) .…”

Section: Introductionmentioning

confidence: 99%

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

Lee¹,

Lee²,

Kim³

et al. 2014

BioResources

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Medium components for β-glucosidase (BGL) production in Schizophyllum commune KUC9397 were optimized using a central composite design and response surface methodology. From the various medium components tested, cellulose, soy peptone, and thiamine HCl were selected as the optimal carbon, nitrogen, and vitamin sources, respectively. The highest BGL production was obtained with 2.96% cellulose, 2.30% soy peptone, and 0.11% thiamine HCl. BGL production in the optimized medium was increased 7.2-fold compared to production in an unoptimized medium. Crude enzyme preparation from S. commune KUC9397 was used to saccharify pretreated lignocellulosic biomass. The crude enzyme preparations showed statistically equal saccharification rates as Cellobiase, a commercial BGL. This finding indicates that crude enzymes produced by S. commune KUC9397 have good potential for application in cellulosic biomass conversion systems in place of Cellobiase.

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Cellulase Production Under Solid-State Fermentation by Trichoderma reesei RUT C30: Statistical Optimization of Process Parameters

Cited by 119 publications

References 22 publications

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Optimization and characterization of cold-active endoglucanase produced by Aspergillus terreus strain AKM-F3 grown on sugarcane bagasse

Optimization of Medium Components for β-glucosidase Production in Schizophyllum commune KUC9397 and Enzymatic Hydrolysis of Lignocellulosic Biomass

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