Cellulases and animal feed production by solid-state fermentation by Aspergillus fumigatus NRCF-122 mutant

Abstract

A high cellulase-producing fungus was isolated from agricultural waste compost, purified, identified genetically as Aspergillus fumigatus NRCF-122 (accession no. MT318341), and improved by UV-irradiation for cellulase production (named as A. fumigatus mutant-17). Optimal cellulase production conditions were studied using sugar beet pulp "SBP" as the substrate of solid-state fermentation "SSF" technique. The optimal conditions were: initial moisture content ratio (1:2 w/v), incubation temperature (30°C), initial pH of solid culture (10), incubation period (4 days), aeration area (1:100 ratio ((1%)), SBP substrate weight : flask volume of moistened SBP in a 250 mL Erlenmeyer flask, fungal inoculum (seeding) culture age (3 days) and inoculum size (20%, v/w). Studies were also involved the effect of different nitrogen sources where urea is the best nitrogen source for cellulase production. Concerning the application of bioethanol production from yeast, using rice straw cellulose as a source of fermentable reducing sugars, the lingo-cellulosic waste rice straw was first pretreated (with NaOH 1.5%) to reduce crystallinity of cellulose & removing lignin and other inhibitors, enhancing the subsequent process (saccharification) of rice straw cellulose. Then, its cellulose was saccharified enzymatically by cellulases (produced from SBP as substrate (under SSF condition) as substrate under the optimum conditions) resulting in reducing sugars. one of the largest livestock production operating costs is the feed bill. In our study we evaluated the resulted crude protein content of the fermented SBP and the results declared that, the total digest nutrient (TDN) and gross energy performance were increased, where as crude fiber, acid detergent fiber ADF & neutral detergent fiber NDF were decreased, thereby upgrading the feed quality of biologically treated SBP.

Design, synthetic approach,in silicomolecular docking and antibacterial activity of quinazolin-2,4-dione hybrids bearing bioactive scaffolds

“…They were cultivated in Mueller Hinton broth at 35 ± 2 °C for 24 h. The antimicrobial activity and MIC were performed as reported by Qader et al (2021). 2,27–36…”

Sustainable Bioethanol Production by Solid State Fermentation: A Systematic Review

“…fumigatus was recorded at the temperature of 30°C, a drop or rise of temperature of 30°C will cause a decline in cellulase production (Fadela et al, 2021). Passadis et al evaluated different temperature for optimum enzymatic sacchari cation of starch and cellulose and the temperature of 35°C is favorable, given that higher energy is required to maintain higher temperature (Passadis et al, 2022).…”

Solvent-Free Synthesis, In Vitro and In Silico Studies of Novel Potential 1,3,4-Thiadiazole-Based Molecules against Microbial Pathogens

“…The obtained solid was washed with water and then ethanol, and eventually was recrystallized from ethanol to give target products 3-7. 5, 110.05, 115.61, 120.96, 122.34, 122.43, 124.70, 125.58, 126.94, 128.82, 128.93, 137.54, 138.94, 147.33, 147.95, 149.54, 155.89, 15626, 164.16; MS: m/z [%]: 445 (M + ), 444 (75), 317 (30), 281 (28) [32,33]. Initially, thiadiazole compounds were screened for their ability to inhibit microbial growth by the agar well method.…”