Cellulases produced by the endophytic fungus Pycnoporus sanguineus (L.) Murrill

Abstract

Cellulases are enzymes that act on the hydrolysis of cellulosic substrates and comprise a complex capable of hydrolyzing cellulose and producing glucose. This conversion can be performed by an enzymatic complex found in secretions of such microorganisms as fungi and bacteria. The challenge is to transform this conversion into a quicker and more cost-effective process. The objective of this study was to evaluate the activity of the cellulase produced by the fungus Pycnoporus sanguineus (L.) Murrill, strains D1-FB, D3-FB, D5-FB and D10-FB, isolated from Baccharis dracunculifolia D. C. (Asteraceae), in the production of cellulolytic enzymes. This study was conducted using sugarcane bagasse enriched with carboxymethylcellulose at 1% as a substrate. The material was kept in an incubator at a temperature ranging from 25 to 45°C for 43 days, with the cellulase activity being quantified every seven days. The indirect quantification method was employed to quantify reducing sugars that were determined by reaction with Domain Name System (DNS). After evaluation, it was observed that the fungus strain P. sanguineus (L.) (D10-FB) presented the highest cellulase activity, with values of 16.32±2.65 IU/g of fermented substrate after 29 days of fermentation, using sugarcane bagasse as the substrate, at a temperature of 30°C and pH 5.5.

In Vitro Antifungal, Enzymatic and Plant Growth Promotion Activities of Endophytic Fungi Associated with Anaphalis Contorta (D.Don) Hook.f. from Manipur

“…Cellulases are widely used in different industries like animal feed, agriculture, laundry and detergent, textile, paper and pulp, brewing, food processing, olive oil extraction, and biofuel production. Cellulase is recorded to be produced by the endophytic isolates Penicillium oxalicum from Taxus cuspidate (Li et al, 2021) and Pycnoporus sanguineus from Baccharis dracunculifolia (Onofre et al, 2015).Laccase is an important enzyme as it can oxidize both toxic and nontoxic substrates and is utilized in the textile industry, food processing industry, wood processing industry, pharmaceutical industry, and chemical industry. Previous studies reported that laccase production by endophytic fungi, Monotospora sp.…”

Optimization of high endoglucanase yields production from polypore fungus, Microporus xanthopus strain KA038 under solid-state fermentation using green tea waste

“…However, because of the higher cost, using a large amount of organic nitrogen source such as yeast extract was not the most efficient method for industrial enzyme production. Our research did not show the best result of endoglucanase production, however, it indicated the potential of polypore fungi M. xanthopus strain KA038 to produce better cellulolytic activity compared with other polypore fungi such as Melanoporia sp., Pycnoporus sanguineus , Pycnoporus coccineus and Coriolus versicolor (Montoya et al, 2015; Onofre et al, 2015; Chuwech et al, 2016; de Oliveira et al, 2016). Thus, this result demonstrated that M. xanthopus was a remarkable species of polypore fungi in producing endoglucanase activity.…”