Cellulases Production Under Solid State Fermentation Using Agro Waste as a Substrate and Its Application in Saccharification by Trametes Hirsuta Ncim

Abstract

Food and energy crisis are the biggest constraint all over the world which has focused lights on need of utilizing renewable resources to meet the future demand. A promising strategy is efficient utilization of lignocellulosic waste and fermentation of the resulting sugars for production of desired metabolites or biofuel. Production of all the cellulase enzymes on wheat bran and different parameters regulating it like pH, moisture ratio (substrate: liquid), temperature and inoculum size has been optimized which found to be 4.5, 1:3, 30°C and 108 spores respectively. Salient feature of partially purified enzyme with stability in the range of 30-50°C under acidic pH range was found to be prominent for industrial applications, moreover in this study, Trametes hirsuta, an efficient cellulase producer, was observed to be an effective species for saccharification of wheat straw to enhance the sugar yield. Enzymatic hydrolysis of wheat straw with 15 FPU of cellulase from the species showed 73% yield in 20 hrs. It may prove to be a suitable choice for the industrial saccharification of lignocellulosic biomasses.

Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review

“…Crude cellulase produced from agricultural residues under SSF has been used in the saccharification of lignocellulosic materials such as rice straw, sugarcane bagasse, corn cob, fruits peel, and wheat bran enhanced the sugar yield for bioethanol production [98][99][100]102,103]. The utilization of grass powder as a carbon source in the production of cellulase and hemicellulase by Phanerochaete chrysosporium by SSF yielded an efficient enzyme complex which could be employed for hydrolysis of rice husk.…”

Bio-processing of Linseed Oil-Cake through Solid State Fermentation by Non- Starch Polysaccharide Degrading Fish Gut Bacteria

“…A fungal strain, Alternaria alternate was accounted for maximum cellulase production at 35°C under SSF [57], which was consistent with our observation. Although, Maurya et al [58] and Bhaumik et al [59] reported 30°C as optimum for maximum cellulase production under SSF by Trichoderma reesei and Trametes hirsute, respectively. Whereas, thermophilic B. licheniformes gave the best result for cellulase production at 50°C under submerged fermentation conditions.…”