Cellules souches mésenchymateuses et médecine régénératrice

Maumus, Marie; Pers, Yves-Marie; Ruiz, Maxime; Jørgensen, Christian; Noël, Danièle

doi:10.1051/medsci/2018294

Cited by 17 publications

(3 citation statements)

References 37 publications

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“…These results were reinforced by a recent meta-analysis including 724 patients, which indicated improvement in pain level measured by Visual Analogue Scale (VAS), International Knee Documentation Committee (IKDC) function score, Tegner Activity Scale and Lysholm Knee score when compared to respective results before treatment with BM-MSCs [ 202 ]. There are also some therapeutic possibilities combining MSCs with biodegradable materials that seem promising but need to be investigated [ 203 ]. It is possible that such regenerative effects are more likely to be observed in the earlier stage and that BM-MSCs have dominantly chondroprotective influence in OA [ 199 ].…”

Section: State-of-the-art Non-operative Therapeutic Considerationmentioning

confidence: 99%

Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations

Primorac

Molnar

Rod

et al. 2020

Genes

285

176

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Being the most common musculoskeletal progressive condition, osteoarthritis is an interesting target for research. It is estimated that the prevalence of knee osteoarthritis (OA) among adults 60 years of age or older is approximately 10% in men and 13% in women, making knee OA one of the leading causes of disability in elderly population. Today, we know that osteoarthritis is not a disease characterized by loss of cartilage due to mechanical loading only, but a condition that affects all of the tissues in the joint, causing detectable changes in tissue architecture, its metabolism and function. All of these changes are mediated by a complex and not yet fully researched interplay of proinflammatory and anti-inflammatory cytokines, chemokines, growth factors and adipokines, all of which can be measured in the serum, synovium and histological samples, potentially serving as biomarkers of disease stage and progression. Another key aspect of disease progression is the epigenome that regulates all the genetic expression through DNA methylation, histone modifications, and mRNA interference. A lot of work has been put into developing non-surgical treatment options to slow down the natural course of osteoarthritis to postpone, or maybe even replace extensive surgeries such as total knee arthroplasty. At the moment, biological treatments such as platelet-rich plasma, bone marrow mesenchymal stem cells and autologous microfragmented adipose tissue containing stromal vascular fraction are ordinarily used. Furthermore, the latter two mentioned cell-based treatment options seem to be the only methods so far that increase the quality of cartilage in osteoarthritis patients. Yet, in the future, gene therapy could potentially become an option for orthopedic patients. In the following review, we summarized all of the latest and most important research in basic sciences, pathogenesis, and non-operative treatment.

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Section: State-of-the-art Non-operative Therapeutic Considerationmentioning

confidence: 99%

Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations

Primorac

Molnar

Rod

et al. 2020

Genes

285

176

View full text Add to dashboard Cite

show abstract

“…MSCs therapy in OA would be appropriate because of their differentiation capacity and paracrine properties [35]. MSCs clinical trials that have been conducted for OA have used different injection methods: intra-articular injection, combination with a scaffold, or combination with platelet-poor plasma [34].…”

Section: Discussionmentioning

confidence: 99%

Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells

et al. 2023

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The objective of the present work was to develop a three-dimensional culture model to evaluate, in a short period of time, cartilage tissue engineering protocols. The spheroids were compared with the gold standard pellet culture. The dental mesenchymal stem cell lines were from pulp and periodontal ligament. The evaluation used RT-qPCR and Alcian Blue staining of the cartilage matrix. This study showed that the spheroid model allowed for obtaining greater fluctuations of the chondrogenesis markers than for the pellet one. The two cell lines, although originating from the same organ, led to different biological responses. Finally, biological changes were detectable for short periods of time. In summary, this work demonstrated that the spheroid model is a valuable tool for studying chondrogenesis and the mechanisms of osteoarthritis, and evaluating cartilage tissue engineering protocols.

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“…Attractively, MSCs can proliferate and differentiate into chondrocytes under endogenous/exogenous signals including matrix, growth factors, proteins, drugs, and mechanical stimuli (Le et al, 2020). MSCs also secrete a secretome of bioactive molecules, i.e., growth factors, prostaglandins, and extracellular vesicles, which modulate the local niche to attenuate inflammation and promote host cell migration, proliferation, differentiation, and matrix deposition (Maumus et al, 2018).…”

Section: Msc Sources For Articular Cartilage Regeneration In Large An...mentioning

confidence: 99%

Updates on mesenchymal stem cell therapies for articular cartilage regeneration in large animal models

Liu

Xiao

et al. 2022

Front. Cell Dev. Biol.

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There is an unmet need for novel and efficacious therapeutics for regenerating injured articular cartilage in progressive osteoarthritis (OA) and/or trauma. Mesenchymal stem cells (MSCs) are particularly promising for their chondrogenic differentiation, local healing environment modulation, and tissue- and organism-specific activity; however, despite early in vivo success, MSCs require further investigation in highly-translatable models prior to disseminated clinical usage. Large animal models, such as canine, porcine, ruminant, and equine models, are particularly valuable for studying allogenic and xenogenic human MSCs in a human-like osteochondral microenvironment, and thus play a critical role in identifying promising approaches for subsequent clinical investigation. In this mini-review, we focus on [1] considerations for MSC-harnessing studies in each large animal model, [2] source tissues and organisms of MSCs for large animal studies, and [3] tissue engineering strategies for optimizing MSC-based cartilage regeneration in large animal models, with a focus on research published within the last 5 years. We also highlight the dearth of standard assessments and protocols regarding several crucial aspects of MSC-harnessing cartilage regeneration in large animal models, and call for further research to maximize the translatability of future MSC findings.

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Cellules souches mésenchymateuses et médecine régénératrice

Cited by 17 publications

References 37 publications

Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations

Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations

Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells

Updates on mesenchymal stem cell therapies for articular cartilage regeneration in large animal models

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