Cellulolytic Bacillus species isolated from the gut of the desert locust Schistocerca gregaria

Khan, Nelson; Muge, Edward K.; Wamalwa, Benson

doi:10.1016/j.sciaf.2020.e00665

Cited by 14 publications

(10 citation statements)

References 34 publications

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“…Interestingly, for Bacillus cereus/thuringiensis UiTM-E2, the highest cellulolytic activity was seen after 48 h of incubation. The cellulolytic index almost doubled at 48 h compared to 24 h. Additionally, the cellulolytic index of both the isolates was found to be higher than that of the reported cellulolytic index of other reported Bacillus species (Apun et al, 2000;Hastuti et al, 2014;Nelson et al, 2021). This indicated a high potential for these two isolated to be utilized as PHA producers using cellulosic materials as carbon sources directly, without having a pretreatment step to break the cellulosic material down to simpler sugars before they can be used to synthesize PHA.…”

Section: Cellulolytic Index Analysismentioning

confidence: 69%

Isolation and Characterization of Polyhydroxyalkanoate (Pha)-Producing, Cellulolytic Bacteria From Municipal Wastewater

ROSS¹,

ZUBIR²,

Razak³

et al. 2022

MAB

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Polyhydroxyalkanoates (PHA) are linear bio-esters produce in nature by certain microorganisms particularly when subjected to limited nutrients sources such as nitrogen while carbon sources are at abundance. PHA has attracted tremendous amount of attention from scientists globally due to their biodegradable property as environment-friendly alternative to petrochemical based plastics. However, the biggest drawback in using PHA is their high production cost. The aims of this study are to assess and characterise PHA-producing bacteria from municipal wastewater and compare their efficiencies in PHA production. A total of 34 out of 282 bacterial isolates from wastewater showed the ability to produce PHA using Sudan Black B staining. These isolates were then subjected to cellulase activity screen using carboxymethyl cellulose (CMC) agar. Two out of the 34 isolates were showed promising cellulose degrading capability and the cellulolytic activities were studied at 24, 48 and 72 hours of incubation respectively. The PHA production efficiencies of both isolates UiTM-E1 and UiTM-E2 were then compared using mineral salt media (MSM) supplemented with 1% and 2% glucose at 24, 48 and 72 hours of incubations respectively. Isolate UiTM-E2 showed the highest PHA production at 36.93% of its cell-dry weight (CDW) at 48 hours incubation.

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Section: Cellulolytic Index Analysismentioning

confidence: 69%

Isolation and Characterization of Polyhydroxyalkanoate (Pha)-Producing, Cellulolytic Bacteria From Municipal Wastewater

ROSS¹,

ZUBIR²,

Razak³

et al. 2022

MAB

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“…Xylanolytic and pectinolytic enzyme activities for Bacillus safensis M35 strain were determined as 15.10 U and 411.58 U, respectively. In a study performed by Nelson et al, (2021), the cellulolytic capacities of Bacillus strains isolated from the intestinal flora of Schistocerca gregaria were investigated. The cellulase enzyme activities of these isolated bacteria were determined by zone clearance assay, and the Index Cellulolytic Activity values (ICA values) of Bacillus safensis strain MED1 and Bacillus safensis strain CACO strains were determined as 1.146 ± 0.109 and 0.8442 ± 0.09203, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…After the staining process, 5mL, 1M NaCl was added in order to remove the dye from the medium and waited for 15 minutes. Yellow zones were observed around microorganisms capable of degrading cellulose and these bacteria were recorded as cellulase positive (Kim et al, 2005;Nelson et al, 2021).…”

Section: Qualitative Determination Of Cellulase Enzyme Activitymentioning

confidence: 99%

Searching for Versatile Polysaccharide-Degrading Alkali-tolerant or Alkaliphilic Bacillus Strains

Bayram

Aydoğan

2022

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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The plant-derived polysaccharides (Cellulose, xylose and amylose, etc.) are the most abundant renewable raw materials in nature. Cellulose and xylose are the predominant carbohydrate polymer components of the plant cell walls and the most abundant biopolymers in the world. Another plant-derived polysaccharide, starch is found in plant tubers, roots and seed endosperms as a major carbohydrate reserve. In this study, it was aimed to find multi-enzyme producer bacteria strains in terms of industrially important enzymes such as cellulase, xylanase and amylase. For this purpose, isolated Bacillus strains from different samples were qualitatively evaluated for cellulase, xylanase and amylase enzyme production potentials. The isolates that have the highest enzyme activity were selected for biochemical tests, molecular and phenotypic characterization. As a result of these characterization process, SB57, SB104, SB155, SB178, SB197 and SB199 strains were identified as Bacillus pumilus and SB118, SB138 strains were identified as Bacillus safensis. In addition to these strains, SB120 and SB147 strains were identified as Bacillus aerius and Bacillus licheniformis respectively. 16S rDNA sequence analysis results of these Bacillus strains were deposited in NCBI GenBank® under accession number KT371465 -KT371474 respectively.

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“…The final result is that the logistic growth represents the best tradeoff between deviation from optimal size and cost of growing of a population over its entire life. Such a functional interpretation of the Verhulst-Pearl equation is certainly much more simple and intuitive than those given by Volterra in 1939 and by Leitmann in 1972, which were not formulated ove an infinite time horizon was used, which assumes substrate saturation conditions during which the specific growth rate is not a function of S, where X M is the value for which dx/dt = 0 for X > 0, as seen in equation 10: (10) For substrate consumption, Pirt expression 34 was used. It considers m as the maintenance coefficient in which S (t) vs X (t) produces a straight line with slope, 1 / Y (X/S) , as in equation 11: (11) For the generated product, the Luedeking-Piret (1959) equation 45 was used, which considers the relationship between microbial growth and product formation, where is product yield based on biomass and k is the destruction factor, as established in equation 12:…”

Section: Selection Of Kinetic Models For Bacillus Subtilis (Bal3) Gro...mentioning

confidence: 99%

“…Numerous microorganisms produce cellobiohydrolases, endoglucanases and other enzymes of interest that degrade lignocellulosic residues 6,10 . Fungi, especially, are increasingly being used in the industry 3 , though bacteria are gaining protagonism in recent years 10,11 Bacteria have a higher growth rate, which warrants the constant search for new strains or species with greater cellulolytic efficiency 6,10 .…”

Section: Introductionmentioning

confidence: 99%

Growth kinetics of Bacillus subtilis (Bal3) for production of cellulolytic crudes used in the bioethanol production industry

et al. 2022

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Biotechnologically manageable microbiota that can produce enzyme complexes of interest is a thriving research field. After careful collection and laboratory culture, the microorganisms can develop hydrolysis and fermentation processes in bioreactors. Kinetic models for their growth are applied, being the most used those of Monod, Moser, Blackman and Powell, which combine mathematical models and experimental work. The objective of this study was to determine the kinetic behavior of Bacillus subtilis (Bal3) to size the equipment needed to obtain enough amount of enzimes to produce 500 hl of ethanol at 70% per day. A medium composed of 60 g of sugarcane bagasse and 350 ml of whey in 1L of water with 0.02 g of inoculum was placed in a 2L Bioflo/CelliGen 115 bioreactor for 130 h at 20° C and 150 rpm. The pH ranged from 6.8 (start) to 5.8 (end). A biomass-substrate yield of 19.34% and a biomass productivity of 0.048 g/L per hour were obtained. With the kinetic models of Velhurs-Pearl, Pirt and Luedeking-Piret, the mass balance was performed and the equipment volume was calculated. Monod's model was considered to adequately represent the growth behavior of the bacteria.

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Cellulolytic Bacillus species isolated from the gut of the desert locust Schistocerca gregaria

Cited by 14 publications

References 34 publications

Isolation and Characterization of Polyhydroxyalkanoate (Pha)-Producing, Cellulolytic Bacteria From Municipal Wastewater

Isolation and Characterization of Polyhydroxyalkanoate (Pha)-Producing, Cellulolytic Bacteria From Municipal Wastewater

Searching for Versatile Polysaccharide-Degrading Alkali-tolerant or Alkaliphilic Bacillus Strains

Growth kinetics of Bacillus subtilis (Bal3) for production of cellulolytic crudes used in the bioethanol production industry

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