Cellulose Acetate Membranes for the Electrophoretic Demonstration of Hemoglobin A&lt;sub&gt;2&lt;/sub&gt;

Petrakis, Nicholas L.; Doherty, Mark; Grunbaum, Benjamin W.; Atchley, William A.

doi:10.1159/000206757

Acta Haematol

1962

DOI: 10.1159/000206757

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Cellulose Acetate Membranes for the Electrophoretic Demonstration of Hemoglobin A<sub>2</sub>

Nicholas L. Petrakis¹,

Mark Doherty²,

Benjamin W. Grunbaum³

et al.

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1965

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Cited by 18 publications

(2 citation statements)

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Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate

Marengo-Rowe¹

1965

Journal of Clinical Pathology

345

129

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Kohn (1958 and1960) and developed by other authors (Petrakis, Doherty, Grunbaum, and Atchley, 1962; Friedman, 1962;Afonso, 1962;Graham and Grunbaum, 1963). These have disadvantages, such as the requirement for special apparatus and staining procedures, or a failure to quantitate the A2 fraction. Bartlett (1963) obtained reproducible values for haemoglobin A2 in a small series of normal persons by eluting this fraction unstained.The following method has been used in this laboratory under routine conditions and has given satisfactory and reproducible results; it is technically simple, requires no staining, and employs apparatus available in most laboratories. Results can be obtained within 90 minutes of receiving a blood sample. APPARATUS AND REAGENTSThe apparatus requires a power pack capable of supplying direct current up to 10 mA at 200 volts, a tank suitable for electrophoresis on cellulose acetate in the horizontal plane,' cellulose acetate strips 5 x 12 cm.,2 and a spectrophotometer with a narrow wavelength band at 413 my; the Unicam SP 600 or the Hilger ultra-violet spectrophotometer are suitable instruments.

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…”

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confidence: 99%

“…Simpler methods employing cellulose acetate membrane as the supporting medium were suggested by Kohn (1958 and1960) and developed by other authors (Petrakis, Doherty, Grunbaum, and Atchley, 1962;Friedman, 1962;Afonso, 1962; Graham and Grunbaum, 1963 …”

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confidence: 99%

Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate

Marengo-Rowe¹

1965

Journal of Clinical Pathology

345

129

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The effect of alteration of intracellular 2,3-DPG concentration upon oxygen binding of intact erythrocytes containing normal and mutant hemoglobins

Lian

Roth

Harkness

1971

Biochemical and Biophysical Research Communications

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A simple method for the quantitation of haemoglobin-A2 by starch gel electrophoresis

Aksoy¹,

Erdem²

1965

Clinica Chimica Acta

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Cellulose Acetate Membranes for the Electrophoretic Demonstration of Hemoglobin A<sub>2</sub>

Cited by 18 publications

References 4 publications

Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate

Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate

The effect of alteration of intracellular 2,3-DPG concentration upon oxygen binding of intact erythrocytes containing normal and mutant hemoglobins

A simple method for the quantitation of haemoglobin-A2 by starch gel electrophoresis

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