Cellulose in Bacterial Biofilms

Serra, Diego O.; Hengge, Regine

doi:10.1007/978-3-030-12919-4_8

Cited by 27 publications

(25 citation statements)

References 114 publications

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“…Exopolysaccharides, which differ in their monosaccharide constituents, chemical modifications, composition and types of glycosidic linkage, can be divided into two functional classes with either aggregative or water-binding mucoid properties. A widely occurring example of aggregative exopolysaccharides, which in general confer cohesion and structural stability to biofilms, is cellulose [27,28,29]. In biofilm matrices of Escherichia coli and many other bacteria, cellulose is present as a phosphoethanolamin-modified derivative (pEtN-cellulose) [30].…”

Section: Bacterial Biofilmsmentioning

confidence: 99%

“…The best-studied biofilm-associated amyloids are curli fibres in E. coli and Salmonella enterica , which also occur in other enterics [41,43,44]. In E. coli , curli fibres are co-regulated with pEtN-cellulose [45,46], with the two fibres tightly associating into a composite material and forming a large-scale matrix architecture within biofilms [24,29]. In many E. coli strains, curli fibres and cellulose are produced below 30 °C only, suggesting a major role in environmental biofilms.…”

Section: Bacterial Biofilmsmentioning

confidence: 99%

“…As bacterial biofilms are highly diverse in their structure and matrix composition, several model systems are currently used in research addressing both the formation and the inhibition of biofilms [29,56]: (i) submerged biofilms that form on the walls of microtiter dishes in which the biofilms’ overall mass can be easily quantified by staining with crystal violet or other dyes, (ii) macrocolonies growing for extended times at a semi-solid surface (agar)/air interface that are highly amenable to genetic approaches, and (iii) pellicles forming at static fluid/air interfaces [56]. In searching for and characterizing anti-biofilm agents, submerged biofilm models have been widely used, which due to the low cost and simplicity of the procedure can also be adapted to high-throughput screening.…”

Section: Bacterial Biofilmsmentioning

confidence: 99%

“…Since these considerations suggested biofilm amyloid formation as a prime candidate for being targeted by EGCG, the action of the compound was tested with macrocolony biofilms of commensal and pathogenic E. coli strains [80], where the wrinkled morphotype depends on the high production of amyloid curli fibres and pEtN-cellulose [24,29]. EGCG was indeed found to essentially eliminate the entire CR-stainable extracellular matrix; i.e., curli fibres as well as pEtN-cellulose (Figure 1).…”

Section: Mechanisms Of Action Of Egcg On Bacterial Biofilmsmentioning

confidence: 99%

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Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG

Hengge

2019

Molecules

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Bacterial biofilms are multicellular aggregates in which cells are embedded in an extracellular matrix of self-produced biopolymers. Being refractory to antibiotic treatment and host immune systems, biofilms are involved in most chronic infections, and anti-biofilm agents are being searched for urgently. Epigallocatechin-3-gallate (EGCG) was recently shown to act against biofilms by strongly interfering with the assembly of amyloid fibres and the production of phosphoethanolamin-modified cellulose fibrils. Mechanistically, this includes a direct inhibition of the fibre assembly, but also triggers a cell envelope stress response that down-regulates the synthesis of these widely occurring biofilm matrix polymers. Based on its anti-amyloidogenic properties, EGCG seems useful against biofilms involved in cariogenesis or chronic wound infection. However, EGCG seems inefficient against or may even sometimes promote biofilms which rely on other types of matrix polymers, suggesting that searching for ‘magic bullet’ anti-biofilm agents is an unrealistic goal. Combining molecular and ecophysiological aspects in this review also illustrates why plants control the formation of biofilms on their surfaces by producing anti-amyloidogenic compounds such as EGCG. These agents are not only helpful in combating certain biofilms in chronic infections but even seem effective against the toxic amyloids associated with neuropathological diseases.

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Section: Bacterial Biofilmsmentioning

confidence: 99%

Section: Bacterial Biofilmsmentioning

confidence: 99%

Section: Bacterial Biofilmsmentioning

confidence: 99%

Section: Mechanisms Of Action Of Egcg On Bacterial Biofilmsmentioning

confidence: 99%

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Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG

Hengge

2019

Molecules

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“…CsgA is secreted to the cell surface as unstructured and soluble monomers (Gibson et al, 2007) that are then templated into ordered amyloid fibres by the nucleator protein CsgB (Hammer et al, 2007;Evans and Chapman, 2014). Curli fibres are produced either alone or in combination with pEtNcellulose by most commensal and pathogenic E. coli strains (Bokranz et al, 2005;Serra et al, 2013a;Thongsomboon et al, 2018;Serra and Hengge, 2019a). As a biofilm master regulator, CsgD activates the expression of genes essential for curli and pEtN-cellulose production during entry into the stationary phase, since CsgD expression depends on the stationary phase sigma subunit of RNA polymerase, RpoS (σ S ), and the second messenger c-di-GMP (Hengge, 2009(Hengge, , 2011.…”

Section: Introductionmentioning

confidence: 99%

Common plant flavonoids prevent the assembly of amyloid curli fibres and can interfere with bacterial biofilm formation

Pruteanu

Lobato

Stach

et al. 2020

Environmental Microbiology

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Like all macroorganisms, plants have to control bacterial biofilm formation on their surfaces. On the other hand, biofilms are highly tolerant against antimicrobial agents and other stresses. Consequently, biofilms are also involved in human chronic infectious diseases, which generates a strong demand for anti-biofilm agents. Therefore, we systematically explored major plant flavonoids as putative antibiofilm agents using different types of biofilms produced by Gram-negative and Gram-positive bacteria. In Escherichia coli macrocolony biofilms, the flavone luteolin and the flavonols myricetin, morin and quercetin were found to strongly reduce the extracellular matrix. These agents directly inhibit the assembly of amyloid curli fibres by driving CsgA subunits into an off-pathway leading to SDS-insoluble oligomers. In addition, they can interfere with cellulose production by still unknown mechanisms. Submerged biofilm formation, however, is hardly affected. Moreover, the same flavonoids tend to stimulate macrocolony and submerged biofilm formation by Pseudomonas aeruginosa. For Bacillus subtilis, the flavonone naringenin and the chalcone phloretin were found to inhibit growth. Thus, plant flavonoids are not general anti-biofilm compounds but show speciesspecific effects. However, based on their strong and direct anti-amyloidogenic activities, distinct plant flavonoids may provide an attractive strategy to specifically combat amyloid-based biofilms of some relevant pathogens.

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Aerogels based on Bacterial Nanocellulose and their Applications

Panahi‐Sarmad,

Alikarami,

Guo

et al. 2024

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Microbial cellulose stands out for its exceptional characteristics in the form of biofilms formed by highly interlocked fibrils, namely, bacterial nanocellulose (BNC). Concurrently, bio‐based aerogels are finding uses in innovative materials owing to their lightweight, high surface area, physical, mechanical, and thermal properties. In particular, bio‐based aerogels based on BNC offer significant opportunities as alternatives to synthetic or mineral counterparts. BNC aerogels are proposed for diverse applications, ranging from sensors to medical devices, as well as thermal and electroactive systems. Due to the fibrous nanostructure of BNC and the micro‐porosity of BNC aerogels, these materials enable the creation of tailored and specialized designs. Herein, a comprehensive review of BNC‐based aerogels, their attributes, hierarchical, and multiscale features are provided. Their potential across various disciplines is highlighted, emphasizing their biocompatibility and suitability for physical and chemical modification. BNC aerogels are shown as feasible options to advance material science and foster sustainable solutions through biotechnology.

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Cellulose in Bacterial Biofilms

Cited by 27 publications

References 114 publications

Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG

Targeting Bacterial Biofilms by the Green Tea Polyphenol EGCG

Common plant flavonoids prevent the assembly of amyloid curli fibres and can interfere with bacterial biofilm formation

Aerogels based on Bacterial Nanocellulose and their Applications

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