Cellulose/soy protein isolate composite membranes: Evaluations of in vitro cytocompatibility with Schwann cells and in vivo toxicity to animals

Luo, Lihua; Gong, Wenrong; Zhou, Yi; Yang, Lin; Li, Daokun; Huselstein, Céline; Wang, Xiong; He, Xiaohua; Li, Yinping; Chen, Yun

doi:10.3233/bme-141228

Cited by 7 publications

(12 citation statements)

References 11 publications

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“…The cellulose/SPIbased films and sponges showed good biocompatibility in vitro and in vivo and have potential for application as biomaterials [37,38]. In addition, the cellulose/SPI films had in vitro cytocompatibility with Schwann cells [40]. It has been confirmed that NGCs fabricated from cellulose/SPIbased films (CSFC) promoted nerve regeneration in a rat model with a 10 mm long sciatic nerve gap [41].…”

Section: Introductionmentioning

confidence: 92%

Cellulose/soy protein composite-based nerve guidance conduits with designed microstructure for peripheral nerve regeneration

Gan¹,

Zhao

et al. 2016

J. Neural Eng.

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Section: Introductionmentioning

confidence: 92%

Cellulose/soy protein composite-based nerve guidance conduits with designed microstructure for peripheral nerve regeneration

Gan¹,

Zhao

et al. 2016

J. Neural Eng.

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“…Solvent casting (molding method) used cellulose/soy protein dissolved in urea/NaOH solution and poured into a polyurethane mold with a central glass rod; the coagulation was carried out with the application of acetic acid [ 23 , 31 ]. Another study used hydroxyethyl cellulose and isolated soy protein dissolved in NaOH solution, and the solution was poured into molds with 1.3 or 1.7 metal rods; through a freeze-drying process, the final tubular shape was achieved [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Gutiérrez,

González-Quijón,

Martínez-Rodríguez

et al. 2024

Polymers

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Background: The elaboration of biocompatible nerve guide conduits (NGCs) has been studied in recent years as a treatment for total nerve rupture lesions (axonotmesis). Different natural polymers have been used in these studies, including cellulose associated with soy protein. The purpose of this report was to describe manufacturing NGCs suitable for nerve regeneration using the method of dip coating and evaporation of solvent with cellulose acetate (CA) functionalized with soy protein acid hydrolysate (SPAH). Methods: The manufacturing method and bacterial control precautions for the CA/SPAH NGCs were described. The structure of the NGCs was analyzed under a scanning electron microscope (SEM); porosity was analyzed with a degassing method using a porosimeter. Schwann cell (SCL 4.1/F7) biocompatibility of cell-seeded nerve guide conduits was evaluated with the MTT assay. Results: The method employed allowed an easy elaboration and customization of NGCs, free of bacteria, with pores in the internal surface, and the uniform wall thickness allowed manipulation, which showed flexibility; additionally, the sample was suturable. The NGCs showed initial biocompatibility with Schwann cells, revealing cells adhered to the NGC structure after 5 days. Conclusions: The fabricated CA/SPAH NGCs showed adequate features to be used for peripheral nerve regeneration studies. Future reports are necessary to discuss the ideal concentration of CA and SPAH and the mechanical and physicochemical properties of this biomaterial.

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“…Schwann cells (SCs) are one of most important glial cells in the neural system and generally-used as seed cells in nerve tissue engineering, which could promote axon regeneration [ [11] , [12] , [13] ]. Schwann cells were found to blossom into bands of Büngner to facilitate axonal regeneration [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

Tong

Luo

et al. 2021

Bioactive Materials

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Peripheral nerve regeneration requires stepwise and well-organized establishment of microenvironment. Since local delivery of VEGF-A in peripheral nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cells (SCs) play critical role in nerve repair, combination of VEGF and Schwann cells may lead to efficient peripheral nerve regeneration. VEGF-A overexpressing Schwann cells were established and loaded into the inner wall of hydroxyethyl cellulose/soy protein isolate/polyaniline sponge (HSPS) conduits. When HSPS is mechanically distorted, it still has high durability of strain strength, thus, can accommodate unexpected strain of nerve tissues in motion. A 10 mm nerve defect rat model was used to test the repair performance of the HSPS-SC (VEGF) conduits, meanwhile the HSPS, HSPS-SC, HSPS-VEGF conduits and autografts were worked as controls. The immunofluorescent co-staining of GFP/VEGF-A, Ki67 and MBP showed that the VEGF-A overexpressing Schwann cells could promote the proliferation, migration and differentiation of Schwann cells as the VEGF-A was secreted from the VEGF-A overexpressing Schwann cells. The nerve repair performance of the multifunctional and flexible conduits was examined though rat behavioristics, electrophysiology, nerve innervation to gastrocnemius muscle (GM), toluidine blue (TB) staining, transmission electron microscopy (TEM) and NF200/S100 double staining in the regenerated nerve. The results displayed that the effects on the repair of peripheral nerves in HSPS-SC (VEGF) group was the best among the conduits groups and closed to autografts. HSPS-SC (VEGF) group exhibited notably increased CD31 + endothelial cells and activation of VEGFR2/ERK signaling pathway in the regenerated nerve tissues, which probably contributed to the improved nerve regeneration. Altogether, the comprehensive strategy including VEGF overexpressing Schwann cells-mediated and HSPS conduit-guided peripheral nerve repair provides a new avenue for nerve tissue engineering.

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Cellulose/soy protein isolate composite membranes: Evaluations of in vitro cytocompatibility with Schwann cells and in vivo toxicity to animals

Cited by 7 publications

References 11 publications

Cellulose/soy protein composite-based nerve guidance conduits with designed microstructure for peripheral nerve regeneration

Cellulose/soy protein composite-based nerve guidance conduits with designed microstructure for peripheral nerve regeneration

Comprehensive Development of a Cellulose Acetate and Soy Protein-Based Scaffold for Nerve Regeneration

Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

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