CELLULOSE SYNTHASE INTERACTIVE1 Is Required for Fast Recycling of Cellulose Synthase Complexes to the Plasma Membrane in Arabidopsis

Lei, Lei; Singh, Abhishek; Bashline, Logan; Li, Shundai; Yingling, Yaroslava G.; Gu, Ying

doi:10.1105/tpc.15.00442

Cited by 51 publications

(68 citation statements)

References 75 publications

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“…The latter possibility is supported by experiments in Arabidopsis showing reduced velocity of YFP-CESA6 particles in the hypocotyls of csi1 mutants Lei et al, 2013). In a subsequent study, Lei et al (2015) showed that CSI1 is also involved in the formation of endocytosis-derived CSC containing vesicles in the vicinity of the plasma membrane. Thus, it is possible that CSC endocytosis defects in the CSI1RNAi could cause shortening of the CMFs.…”

Section: Discussionmentioning

confidence: 84%

CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

et al. 2020

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SUMMARY Cellulose microfibrils synthesized by CELLULOSE SYNTHASE COMPLEXES (CSCs) are the main load‐bearing polymers in wood. CELLULOSE SYNTHASE INTERACTING1 (CSI1) connects CSCs with cortical microtubules, which align with cellulose microfibrils. Mechanical properties of wood are dependent on cellulose microfibril alignment and structure in the cell walls, but the molecular mechanism(s) defining these features is unknown. Herein, we investigated the role of CSI1 in hybrid aspen (Populus tremula × Populus tremuloides) by characterizing transgenic lines with significantly reduced CSI1 transcript abundance. Reduction in leaves (50–80%) caused leaf twisting and misshaped pavement cells, while reduction (70–90%) in developing xylem led to impaired mechanical wood properties evident as a decrease in the elastic modulus and rupture. X‐ray diffraction measurements indicate that microfibril angle was not impacted by the altered CSI1 abundance in developing wood fibres. Instead, the augmented wood phenotype of the transgenic trees was associated with a reduced cellulose degree of polymerization. These findings establish a function for CSI1 in wood mechanics and in defining leaf cell shape. Furthermore, the results imply that the microfibril angle in wood is defined by CSI1 independent mechanism(s).

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Section: Discussionmentioning

confidence: 84%

CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

et al. 2020

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“…A linked annotated gene, namely Medtr8g091470 (S1 Table), which is known to encode for a cellulose synthase interactive protein, was identified in that chromosome region by the presence of two SNPs in the same exon. This gene is similar (78% protein identity) to a cellulose synthase interactive protein in Arabidopsis (At2g22125) involved in fast recycling of cellulose synthase complexes [70]. This and other associated genes closely located on chromosome 8 (S1 Table) may deserve further study to verify their role as QTL for leaf lignin content control.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Mapping and Genomic Selection for Alfalfa (Medicago sativa) Forage Quality Traits

Biazzi¹,

N.²,

Pecetti³

et al. 2017

PLoS ONE

128

114

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Genetic progress for forage quality has been poor in alfalfa (Medicago sativa L.), the most-grown forage legume worldwide. This study aimed at exploring opportunities for marker-assisted selection (MAS) and genomic selection of forage quality traits based on breeding values of parent plants. Some 154 genotypes from a broadly-based reference population were genotyped by genotyping-by-sequencing (GBS), and phenotyped for leaf-to-stem ratio, leaf and stem contents of protein, neutral detergent fiber (NDF) and acid detergent lignin (ADL), and leaf and stem NDF digestibility after 24 hours (NDFD), of their dense-planted half-sib progenies in three growing conditions (summer harvest, full irrigation; summer harvest, suspended irrigation; autumn harvest). Trait-marker analyses were performed on progeny values averaged over conditions, owing to modest germplasm × condition interaction. Genomic selection exploited 11,450 polymorphic SNP markers, whereas a subset of 8,494 M. truncatula-aligned markers were used for a genome-wide association study (GWAS). GWAS confirmed the polygenic control of quality traits and, in agreement with phenotypic correlations, indicated substantially different genetic control of a given trait in stems and leaves. It detected several SNPs in different annotated genes that were highly linked to stem protein content. Also, it identified a small genomic region on chromosome 8 with high concentration of annotated genes associated with leaf ADL, including one gene probably involved in the lignin pathway. Three genomic selection models, i.e., Ridge-regression BLUP, Bayes B and Bayesian Lasso, displayed similar prediction accuracy, whereas SVR-lin was less accurate. Accuracy values were moderate (0.3–0.4) for stem NDFD and leaf protein content, modest for leaf ADL and NDFD, and low to very low for the other traits. Along with previous results for the same germplasm set, this study indicates that GBS data can be exploited to improve both quality traits (by genomic selection or MAS) and forage yield.

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“…isoxaben) or osmotic stress (Crowell et al, 2009;Gutierrez et al, 2009). Under these conditions, CSCs were mostly depleted from the PM and internalized into MASCs, which were tethered to cortical microtubules and displayed stationary or steady microtubule tip-tracking motility with an average velocity of ;4 mm min 21 (Crowell et al, 2009;Gutierrez et al, 2009;Sampathkumar et al, 2013;Lei et al, 2015). By contrast, the accumulated CESA compartments we observed in myosin-and actin-deficient cells exhibited high dynamicity (Supplemental Movie S3).…”

Section: Cesa Compartments Accumulate In the Cortical Cytoplasm Aftermentioning

confidence: 94%

Myosins XI Are Involved in Exocytosis of Cellulose Synthase Complexes

2019

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In plants, cellulose is synthesized at the cell surface by plasma membrane (PM)-localized cellulose synthase (CESA) complexes (CSCs). The molecular and cellular mechanisms that underpin delivery of CSCs to the PM, however, are poorly understood. Cortical microtubules have been shown to interact with CESA-containing compartments and mark the site for CSC delivery, but are not required for the delivery itself. Here, we demonstrate that myosin XI and the actin cytoskeleton mediate CSC delivery to the PM by coordinating the exocytosis of CESA-containing compartments. Measurement of cellulose content indicated that cellulose biosynthesis was significantly reduced in a myosin xik xi1 xi2 triple-knockout mutant. By combining genetic and pharmacological disruption of myosin activity with quantitative live-cell imaging, we observed decreased abundance of PMlocalized CSCs and reduced delivery rate of CSCs in myosin-deficient cells. These phenotypes correlated with a significant increase in failed vesicle secretion events at the PM as well as an abnormal accumulation of CESA-containing compartments at the cell cortex. Through high-resolution spatiotemporal assays of cortical vesicle behavior, we identified defects in CSC vesicle tethering and fusion at the PM. Furthermore, disruption of myosin activity reduced the delivery of several other secretory markers to the PM and reduced constitutive and receptor-mediated endocytosis. These findings reveal a previously undescribed role for myosin in vesicle secretion and cellulose production at the cytoskeleton-PM-cell wall nexus.

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CELLULOSE SYNTHASE INTERACTIVE1 Is Required for Fast Recycling of Cellulose Synthase Complexes to the Plasma Membrane in Arabidopsis

Cited by 51 publications

References 75 publications

CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

CELLULOSE SYNTHASE INTERACTING 1 is required for wood mechanics and leaf morphology in aspen

Genome-Wide Association Mapping and Genomic Selection for Alfalfa (Medicago sativa) Forage Quality Traits

Myosins XI Are Involved in Exocytosis of Cellulose Synthase Complexes

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