Cementoblast response to low- and high-intensity ultrasound

Dalla-Bona, Diego; Tanaka, Eiji; Inubushi, Toshihiro; Oka, Hiroko; Ohta, Atsumi; Okada, Haruhisa; Miyauchi, Mutsumi; Takata, Takashi; Tanne, Kazuo

doi:10.1016/j.archoralbio.2007.11.006

Cited by 58 publications

(38 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may be due to the anabolic effect of LIPUS on cementoblasts that either helped repair RL or stimulated secretion of a protective cementum layer against OIRR. 16,23,24,29 In addition, it has been demonstrated that LIPUS can inhibit osteoclast activity by decreasing the Receptor activator of nuclear factor kappa-B ligand/Osteoprotegerin (RANKL/OPG) ratio. 23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells.…”

Section: Discussionmentioning

confidence: 99%

“…16,23,24,29 In addition, it has been demonstrated that LIPUS can inhibit osteoclast activity by decreasing the Receptor activator of nuclear factor kappa-B ligand/Osteoprotegerin (RANKL/OPG) ratio. 23,24,30 This could also be due to the anti-inflammatory effect of LIPUS, 31 as it has been reported to decrease the tumor necrosis factor-a or interleukin-1b 31 that are also involved in the proliferation and differentiation of odontoclast cells. 32 Our findings are in accordance with previous reports 15,25,30,31 that showed significantly fewer RL in LIPUS-treated teeth compared to control teeth, except for at the distal surface.…”

Section: Discussionmentioning

confidence: 99%

“…15 LIPUS is acoustic pressure waves that can stimulate a variety of cells including cementoblasts, 16 odontoblasts, 17 osteoblasts, 18 chondrocytes, 19 gingival cells, 20,21 and periodontal ligament cells. 22 Previous studies investigating the effects of LIPUS on OIRR have reported that LIPUS promotes cementogenesis by increasing the Alkaline phosphate (ALP) activity, 16,23 collagen-I synthesis, 16 and Runx-2 protein 16 in cementoblasts and inhibits cementoclastogenesis 24,25 In addition, a previous study 15 reported that LIPUS reduced the severity of OIRR due to tipping-type orthodontic tooth movement and promoted cementum repair. The effect of LIPUS on OIRR during other types of orthodontic tooth movement remains unknown.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

View full text Add to dashboard Cite

Objectives: To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced tooth root resorption caused by torque in human subjects. Materials and Methods: Ten healthy patients (12-35 years of age) who required extraction of all first premolars as a part of their routine orthodontic treatment were recruited. A 15u twist was applied in the arch wire using 0.019 3 0.025-inch TMA in a 0.022-inch bracket system (Synergy R) that produced a buccal root torque of approximately 5 N/mm at the bracket level. Using a split mouth design, randomization, and blinding, one side of the arch received LIPUS for 20 minutes per day for 4 weeks at an incident intensity of 30 mW/cm 2 of the transducers' surface area. The other side served as a self-control, which received a sham transducer. After 4 weeks, all first premolars were extracted and micro-computed tomographic analysis was performed on these extracted teeth. A linear mixed-model statistical analysis was used. Results: LIPUS-treated teeth showed significantly less total volume of resorption lacunae compared to control teeth by a mean difference of (0.54 6 0.09 mm 3 ) (P , .001) and percentage of root resorption by a mean difference of (0.33 6 0.05 mm 3 ) (P , .001). In addition, significantly fewer resorption lacunae were found on all root surfaces in the LIPUS group compared to the control except in the instance of the distal surface. Limitations: This study was performed on limited number of cases during a 4-week period. Conclusions: LIPUS minimizes root resorption when applied during torque tooth movement over a 4-week period. (Angle Orthod. 2016;86:550-557.)

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Raza

Major

Dederich

et al. 2015

The Angle Orthodontist

View full text Add to dashboard Cite

show abstract

“…Our results were similar to those of Lau et al, 7 who showed that low-magnitude, highfrequency vibrations decrease RANKL expression with increased frequency, but they applied the mechanical vibrations to osteocytes rather than to the osteoblastic cell line. 7 Similarly, Dalla-Bona et al 21 treated cementoblasts with high-intensity (150 mW/cm 2 ) and low-intensity (30 mW/cm 2 ) ultrasonic radiation (lowintensity, pulsed ultrasound) and found that both high and low intensity increased the OPG expression but did not affect RANKL expression. Our results were similar to those of Dalla-Bona et al 21 with respect to OPG expression, but in our research there was a decrease in RANKL expression, and the observed difference may be due to differences in cell type and mechanical vibration applied.…”

Section: Discussionmentioning

confidence: 99%

The effect of mechanical vibration on orthodontically induced root resorption

Yadav

Dobie

Assefnia

et al. 2016

The Angle Orthodontist

View full text Add to dashboard Cite

Objective: To investigate the effect of low-frequency mechanical vibration (LFMV) on orthodontically induced root resorption. Materials and Methods: Forty male CD1, 12-week-old mice were used for the study. The mice were randomly divided into five groups: group 1 (baseline)-no spring and no mechanical vibration, group 2-orthodontic spring but no vibration, group 3-orthodontic spring and 5 Hz of vibration applied to the maxillary first molar, group 4-orthodontic spring and 10 Hz of vibration applied to maxillary first molar, and group 5-orthodontic spring and 20 Hz of vibration applied to maxillary first molar. In the different experimental groups, the first molar was moved mesially for 2 weeks using a nickel-titanium coil spring delivering 10 g of force. LFMVs were applied at 5 Hz, 10 Hz, and 20 Hz. Microfocus X-ray computed tomography imaging was used to analyze root resorption. Additionally, to understand the mechanism, we applied LFMV to MC3T3 cells, and gene expression analyses were done for receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG). Results: Orthodontic tooth movement leads to decreased root volume (increased root resorption craters). Our in vivo experiments showed a trend toward increase in root volume with different frequencies of mechanical vibration. In vitro gene expression analyses showed that with 20 Hz of mechanical vibration, there was a significant decrease in RANKL and a significant increase in OPG expression. Conclusion: There was a trend toward decreased root resorption with different LFMVs (5 Hz, 10 Hz, and 20 Hz); however, it was not more statistically significant than the orthodontic-springonly group. (Angle Orthod. 2016;86:740-745.)

show abstract

“…More recently, LIPUS has been shown to enhance chondrocyte proliferation in three-dimensional collagen scaffolds (Kobayashi et al, 2009). Another precursor cell proliferation and differentiation that has been recently reported to be stimulated by LIPUS are human periodontal ligament cells that have been differentiated into cementoblasts (teeth root outer layer cells) (Inubushi et al, 2008). This is an important report on the potential use of LIPUS in dental tissue engineering.…”

Section: Lipus Utilization In Cell Expansionmentioning

confidence: 99%

Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

El‐Bialy¹

2010

Tissue Engineering

View full text Add to dashboard Cite

This chapter will focus on the current and future applications of low intensity pulsed ultrasound (LIPUS) in tissue engineering at laboratory and clinical levels. Also, this chapter will explain the molecular basis of LIPUS stimulatory effects on cell expansion, differentiation and matrix production of different tissues. In addition, this chapter will provide information about the differences between LIPUS application in the laboratory settings and in the clinical applications with emphasis on tissue engineering applications. Moreover, this chapter will discuss why variability in outcome might occur when applying LIPUS in laboratory or clinical settings. Also, we will provide some tips on how to prevent potential inconsistency in treatment results with emphasis on the technique sensitivity and how a scientist or a clinician should be aware of this sensitivity of the application technique in order to optimize the utilization of LIPUS in different settings. This chapter will set a few hypotheses and potential new applications of utilizing LIPUS in other tissue engineering applications. The outcome of this chapter will be an understanding of the advantages and limitations of using LIPUS in tissue engineering applications either at the laboratory or in clinical settings.

show abstract

Cementoblast response to low- and high-intensity ultrasound

Cited by 58 publications

References 22 publications

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

Effect of low-intensity pulsed ultrasound on orthodontically induced root resorption caused by torque: A prospective, double-blind, controlled clinical trial

The effect of mechanical vibration on orthodontically induced root resorption

Low Intensity Pulsed Ultrasound: A Laboratory and Clinical Promoter in Tissue Engineering Abstract

Contact Info

Product

Resources

About