Cementogenesis is inhibited under a mechanical static compressive force via Piezo1

Zhang, Yingying; Huang, Yiping; Zhao, Huaxiang; Zhang, Ting; Chen, Feng; Liu, Yan

doi:10.2319/110616-799.1

Cited by 21 publications

(16 citation statements)

References 25 publications

(30 reference statements)

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“…On the other hand, in in vitro models, compressive forces are applied by centrifugation [59][60][61] or the cells are covered with a glass slide and a defined weight of steel is deposited on it (▶fig. 1c) [62]. The usual unit to describe the level of compression or tension is strain and it is defined as the ratio of the length change to the initial length, more precisely 200 µstrain is equal to 2 % length change of the cell [63].…”

Section: Mechanical Stress Applicationsmentioning

confidence: 99%

“…This cell line expresses all required cell-specific proteins (see above) [6,22,57,58,64]. Moreover, the cuboidal morphology of these cells is not affected by mechanical loading [6,62,65,66].…”

Section: Influence Of Mechanical Loading On Gene Expressionmentioning

confidence: 99%

“…Furthermore, RUNX2, ALP and BSP are reduced significantly after 8 hours of compression [64] and also after up to 21 days [66]. Lastly, OPN is proven as being decreased after up to 24 hours in the same compressive conditions [62]. To conclude, cementogenesis is strongly affected by compressive forces [66,67] whereby it remains to be elucidated if there are tissue-specific molecular mechanisms involved in orthodontic root repair [6].…”

Section: Influence Of Mechanical Loading On Gene Expressionmentioning

confidence: 99%

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Mechanical Loading on Cementoblasts: A Mini Review

et al. 2022

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Orthodontic treatments are concomitant with mechanical forces and thereby cause teeth movements. The applied forces are transmitted to the tooth root and the periodontal ligaments which is compressed on one side and tensed up on the other side. Indeed, strong forces can lead to tooth root resorption and the crown-to-tooth ratio is reduced with the potential for significant clinical impact. The cementum, which covers the tooth root, is a thin mineralized tissue of the periodontium that connects the periodontal ligament with the tooth and is build up by cementoblasts. The impact of tension and compression on these cells is investigated in several in vivo and in vitro studies demonstrating differences in protein expression and signaling pathways. In summary, osteogenic marker changes indicate that cyclic tensile forces support whereas static tension inhibits cementogenesis. Furthermore, cementogenesis experiences the same protein expression changes in static conditions as static tension, but cyclic compression leads to the exact opposite of cyclic tension. Consistent with marker expression changes, the singaling pathways of Wnt/ß-catenin and RANKL/OPG show that tissue compression leads to cementum degradation and tension forces to cementogenesis. However, the cementum, and in particular its cementoblasts, remain a research area which should be explored in more detail to understand the underlying mechanism of bone resorption and remodeling after orthodontic treatments.

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Section: Mechanical Stress Applicationsmentioning

confidence: 99%

Section: Influence Of Mechanical Loading On Gene Expressionmentioning

confidence: 99%

Section: Influence Of Mechanical Loading On Gene Expressionmentioning

confidence: 99%

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Mechanical Loading on Cementoblasts: A Mini Review

et al. 2022

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“…3,4 Cementoblasts are sensitive to compressive force and play a crucial role in the regulation of cementum resorption and repair. [5][6][7][8] Therefore, a better understanding of how cementoblasts perform under compressive force is essential for developing new approaches to reduce root resorption during orthodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

“…Compressive force directly induces external root resorption, which occurs as a result of abnormal cementum remodeling during orthodontic tooth movement 3,4 . Cementoblasts are sensitive to compressive force and play a crucial role in the regulation of cementum resorption and repair 5–8 . Therefore, a better understanding of how cementoblasts perform under compressive force is essential for developing new approaches to reduce root resorption during orthodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

Compressive force regulates cementoblast migration via downregulation of autophagy

Yang

Huang

Líu

et al. 2021

Journal of Periodontology

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Background: Migration of cementoblasts to resorption lacunae is the foundation for repairing root resorption during orthodontic tooth movement. Previous studies reported that autophagy was activated by compression in periodontal ligament cells. The aim of this study was to investigate the migration of cementoblasts and determine whether autophagy is involved in the regulation of cementoblast migration under compressive force.Methods: Flow cytometry was employed to examine the apoptosis of murine cementoblasts (OCCM-30) at different compression times (0, 6, 12, and 24 hours) and magnitudes (0, 1.0, 1.5, and 2.0 g/cm 2 ). Cell proliferation was examined using the CCK-8 method. Wound healing migration assays and transwell migration assays were performed to compare the migration of cementoblasts. Chloroquine (CQ) and rapamycin were used to inhibit and activate autophagy, respectively. The level of autophagy was determined using western blotting and immunofluorescence staining. The expression of matrix metalloproteinases (MMPs) was assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blot analysis, and enzyme-linked immunosorbent assay (ELISA). Results: Cell apoptosis and proliferation did not significantly change in OCCM-30 cells under mechanical compression at magnitude of 1.5 g/cm 2 for 12 hours. However, the migration of cementoblasts was significantly inhibited after the application of compressive force. MMP2, MMP9, and MMP13 mRNA expression was decreased, and MMP9 and MMP13 protein expression and secretion level were also decreased. Further, autophagic activity was inhibited in cementoblasts under compressive force. Treatment with chloroquine reduced the cellular migration, and rapamycin partially relieved the inhibition of cementoblast migration induced by the compressive force. MMP9 and MMP13 mRNA expression, protein expression, and secretion levels showed a similar trend. Conclusion:Migration of OCCM-30 cells was inhibited under compressive force partially dependent on the inhibition of MMPs, which was mediated by downregulation of autophagy. The findings provide new insights into the role of e128

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Autophagy mediates cementoblast mineralization under compression through periostin/β‐catenin axis

Yang

Líu

Wang

et al. 2023

Journal Cellular Physiology

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Repair of orthodontic external root resorption and periodontal tissue dysfunction induced by mechanical force remains a clinical challenge. Cementoblasts are vital in cementum mineralization, a process important for restoring damaged cementum. Despite autophagy plays a role in mineralization under various environmental stimuli, the underlying mechanism of autophagy in mediating cementoblast mineralization remains unclear. Here we verified that murine cementoblasts exhibit compromised mineralization under compressive force. Autophagy was indispensable for cementoblast mineralization, and autophagic activation markedly reversed cementoblast mineralization and prevented cementum damage in mice during tooth movement. Subsequently, messenger RNA sequencing analyses identified periostin (Postn) as a mediator of autophagy and mineralization in cementoblasts. Cementoblast mineralization was significantly inhibited following the knockdown of Postn. Furthermore, Postn silencing suppressed Wnt signaling by modulating the stability of β‐catenin. Together our results highlight the role of autophagy in cementoblast mineralization via Postn/β‐catenin signaling under compressive force and may provide a new strategy for the remineralization of cementum and regeneration of periodontal tissue.

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Cementogenesis is inhibited under a mechanical static compressive force via Piezo1

Abstract: Cementogenic activity was inhibited in OCCM-30 cells under static mechanical force, a process that was partially mediated by the decrease of Piezo1. This study provides a new viewpoint of the pathogenesis mechanism of orthodontically induced root resorption and repair.

Cited by 21 publications

References 25 publications

Mechanical Loading on Cementoblasts: A Mini Review

Mechanical Loading on Cementoblasts: A Mini Review

Compressive force regulates cementoblast migration via downregulation of autophagy

Autophagy mediates cementoblast mineralization under compression through periostin/β‐catenin axis

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