CENPO regulated proliferation and apoptosis of colorectal cancer in a p53-dependent manner

Liu, Zhicheng; Chen, Chuangqi; Yan, Mingxia; Zeng, Xiangzhong; Zhang, Yuchao; Lai, Dongmei

doi:10.1007/s12672-022-00469-2

Cited by 11 publications

(14 citation statements)

References 42 publications

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“…Studies have reported that the knockdown of CENPO inhibited GC cell growth, induced apoptosis, and decreased the expression of ATM, cyclin D1, and c-Jun [12]. Knockdown of CENPO in CRC downregulated the expression of N-cadherin, Vimentin, Snail, and CCND1, inhibited cell proliferation, and attenuated migration and invasion [15]. This is consistent with our Bioinformatics Analysis, the functional enrichment results showed signi cant enrichment of cell cycle, P53 pathway, and extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

“…High CENPO expression promotes the proliferation of gastric cancer cells and is positively associated with poor prognosis in gastric cancer [12]. CENPO is highly expressed in CRC tissues and cells as positively associated with the malignancy of CRC degree and positively correlated with tumor progression possibly through EMT and PI3K/AKT signaling pathways [15]. CENPO upregulation promoted bladder cancer progression [16].…”

Section: Introductionmentioning

confidence: 99%

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Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Wang¹,

Liu³

et al. 2023

Preprint

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Background The centromere protein O (CENPO) is an important member of the centromere protein family. However, the role of CENPO in pan–cancer and immune infiltration has not been reported. Here, we investigated the role of CENPO in pan–cancer and further validated its role in lung adenocarcinoma (LUAD) by in vitro experiments. Method The UCSC Xena database and The Cancer Genome Atlas (TCGA)–LUAD data were used to assess the mRNA expression levels of CENPO. The potential value of CENPO as a diagnostic and prognostic biomarker for pan–cancer was evaluated using TCGA data and the GEPIA database. The mRNA expression profiles of LUAD patients and the corresponding clinical data were downloaded for correlation analysis. The role of CENPO in immune infiltration was investigated using the UCSC Xena database. Subsequently, RT–QPCR was performed to detect the expression of CENPO. Cell proliferation, migration, and invasion were determined using CCK–8, wound–healing assay, and transwell assay, respectively. Results CENPO is highly expressed in most cancers, and the upregulation of CENPO is associated with poor prognosis in many cancers. CENPO expression correlates with age, TNM stage, N stage, T stage, and receipt of radiotherapy in LUAD patients, and LUAD patients with high CENPO expression have poorer overall survival (OS) and disease–free survival (DFS). In addition, CENPO expression is associated with immune cell infiltration and immune checkpoint inhibitors. Moreover, the expression of CENPO was closely related to the expression of tumor mutational load and microsatellite instability. In vitro experiments showed that CENPO expression was increased in LUAD cell lines and that knockdown of CENPO significantly inhibited the proliferation, cell invasion, and migration ability of LUAD cells. Conclusion CENPO may be a potential pan–cancer biomarker and oncogene, especially in LUAD. In addition, CENPO is associated with immune cell infiltration and may serve as a new molecular therapeutic target and effective prognostic marker for LUAD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Wang¹,

Liu³

et al. 2023

Preprint

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“…[ 34 ] Studies have also shown that upregulation of CENPO may lead to bladder cancer and may depend on P53 to regulate the development, proliferation and apoptosis of colorectal cancer. [ 35 , 36 ] Studies have suggested that the high expression of CENPO has an adverse effect on the survival of breast cancer and leads to poor prognosis. It is also considered that CENPO is an independent factor affecting the distant recurrence survival rate of breast cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Gene mining of immune microenvironment in hepatocellular carcinoma

Xu¹

2022

Medicine

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Background: Hepatocellular carcinoma (HCC) is a common malignant tumor worldwide with a poor prognosis. Recent studies have shown that the occurrence, development and prognosis of liver cancer are closely related to tumor microenvironment (TME) and tumor immune infiltration.Methods: Therefore, important information on various diseases can be obtained from public databases such as The Cancer Gene Atlas (TCGA), and ideas or schemes that may be effective for the treatment of various diseases can be screened and analyzed by screening various conditions. In this study, 424 cases of liver hepatocellular carcinoma (LIHC) in the TCGA database and CIBERSORT algorithm were used to calculate the proportion of tumor-invasive immune cells. Combined with the clinical data from TCGA database, it was concluded that T cells regulatory (Tregs) were correlated with the development and prognosis of HCC. Cox regression analysis was used to screen differentially expressed genes, and survival analysis was performed according to the screened differentially expressed genes to see whether there was a significant association with the prognosis of HCC. Then gene ontology and kyoto encyclopedia of genes and genomes analysis of differentially expressed genes were carried out to explore the possibility of differentially expressed genes becoming potential therapeutic targets of HCC.Results: Finally, I identified the gene centromere protein o (CENPO), which is associated with immune cells and improve the prognosis of HCC.Conclusion: CENPO may be a potential biological therapeutic target for hepatocellular treatment.

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“…Deletion of the CENPO protein results in aneuploidy and aneuploidy chromosomal gains, which can lead to the development of disease or cancer [ 9 ]. Recent studies have shown that the alteration of CENPO expression has been found to contribute to the development of gastric cancer and colorectal cancer [ 10 , 11 ]. The mechanism of CENPO in bladder cancer may involve mitosis and complement as well as coagulation cascade signaling pathways [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Pan-cancer landscape of CENPO and its underlying mechanism in LUAD

Shi

Tian

et al. 2023

Respir Res

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Background Centromere protein O (CENPO) is a newly discovered constitutive centromeric protein, associated with cell death. However, little is known about how CENPO expression is associated with human cancers or immune infiltration. Here, we assessed the function of CENPO in pan-cancer and further verified the results in lung adenocarcinoma (LUAD) through in vitro and in vivo experiments. Methods Sangerbox and TCGA databases were used to evaluate the CENPO expression level in different human cancer types. A subsequent evaluation of the potential role of CENPO as a diagnostic and prognostic biomarker in pancancer was conducted. The CENPO mutations were analyzed using the cBioPortal database and its function was analyzed using the LinkedOmics and CancerSEA databases. The TIMER2 and TISIDB websites were used to find out how CENPO affects immune infiltration. The expression level of CENPO in LUAD was revealed by TCGA database and immunohistochemical (IHC) staining. Targetscan, miRWalk, miRDB, miRabel, LncBase databases, and Cytoscape tool were used to identify microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) that regulate expression and construct ceRNA network. Subsequently, loss-of-function assays were performed to identify the functions of CENPO on the malignant behavior and tumor growth of LUAD in vitro and in vivo experiments. Results In most cancers, CENPO was upregulated and mutated, which predicted a poorer prognosis. Furthermore, infiltration of CENPO and myeloid-derived suppressor cells (MDSC) showed a significant positive correlation, while T-cell NK infiltration showed a significant negative correlation in most cancers. CENPO was expressed at high levels in LUAD and was correlated with p-TNM stage. Furthermore, CENPO knockdown suppressed the malignant phenotypes of LUAD cells, manifested by slower proliferation, cycle in G2, increased apoptosis, decreased migration, and attenuated tumorigenesis. Furthermore, CENPO knockdown decreased CDK1/6, PIK3CA, and inhibited mTOR phosphorylation, suggesting that the mTOR signaling pathway may be involved in CENPO-mediated regulation of LUAD development. Conclusions In pan-cancer, especially LUAD, CENPO may be a potential biomarker and oncogene. Furthermore, CENPO has been implicated in immune cell infiltration in pan-cancer and represents a potential immunotherapeutic target for tumor therapy.

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CENPO regulated proliferation and apoptosis of colorectal cancer in a p53-dependent manner

Cited by 11 publications

References 42 publications

Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Prognostic and immune infiltrative biomarkers of CENPO in pan- cancer and its relationship with lung adenocarcinoma cell proliferation and metastasis

Gene mining of immune microenvironment in hepatocellular carcinoma

Pan-cancer landscape of CENPO and its underlying mechanism in LUAD

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