Centella asiatica (L.) Urban, (Pennywort): Cell Culture, Production of Terpenoids, and Biotransformation Capacity

Solet, Jean-Michel; Simon-Ramiasa, A.; Cosson, L.; Guignard, Jean-Louis

doi:10.1007/978-3-642-58833-4_6

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…1988). The bioactive triterpenes of C. asiatica include a pair of saponins, asiatic acid and madecassic acid, and a pair of trisaccharides, asiaticoside and madecassoside (Schaneberg et al, 2003;Solet et al, 1998). Marquart et al (1990) reported a positive correlation between a mixture of three terpenoids from C. asiatica and the collagen synthesis in human foreskin fibroblasts.…”

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confidence: 99%

Somatic Embryogenesis and Plant Development in Centella asiatica L., a Highly Prized Medicinal Plant of the Tropics

Joshee¹,

Biswas²,

Yadav³

2007

horts

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Past research experience with Centella asiatica micropropagation suggests a very high rate of contamination during the culture establishment stage. We demonstrate protocols for successful sterilization of Centella explants prepared from field-grown plants with an abundance of fungal and bacterial contamination. Sequential steps during sterilization and explant preparation process included a dip for 30 s in 70% ethyl alcohol, weak bleach treatment for 12 min, and a 60-min soak in plant preservative mixture before establishing cultures. We also report a reproducible system for somatic embryogenesis in Centella using leaf and stolon tip explants collected from naturally growing populations. Somatic embryos were induced within 3 to 4 weeks of culture in the dark on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Initial embryogenic mass appeared as nodular callus, which eventually developed into actual somatic embryos exhibiting globular, heart-shaped, and cotyledonary stages. Leaves produced embryogenic calli at 2.26 and 4.52 μm 2,4-D, whereas stolon tips were responsive only in the 9.04 μm 2,4-D treatment. Withdrawal of 2,4-D/growth regulators from the induction medium resulted in the maturation and further development of the embryos into plantlets. Regular subculturing of the embryogenic calli into MS medium sustained their regenarability for more than 1 year. Somatic embryos were individually encapsulated in sodium alginate and calcium chloride-based encapsulation matrix to produce artificial or synthetic seeds (synseeds). Synseeds with 2% sodium alginate were found best for the survival and germination recorded after their storage at 5 to 8 °C for 30 and 60 days. We report protocols for C. asiatica to reduce explant contamination before establishment of cultures on somatic embryo induction medium and efficient somatic embryogenesis to facilitate conservation and mass production of elite germplasm. This may further assist rapid dissemination of superior clones needed for research and commercial production.

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confidence: 99%

Somatic Embryogenesis and Plant Development in Centella asiatica L., a Highly Prized Medicinal Plant of the Tropics

Joshee¹,

Biswas²,

Yadav³

2007

horts

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“…The pennywort seedlings (variety: Asiatic pennywort) were germinated using tissue culture following the protocol [53,54] and then grown in a greenhouse for one month in the perlite soil mixture pots. After that, the seedlings were separated from the cultivation pots, cleaned, and moved to the DFT system under fluorescent lights to adapt to the hydroponic system and ambient environment of the plant factory as Shown in Figure 2.…”

Section: Pennywort Seedling Preparation and Transplantationmentioning

confidence: 99%

Leaf Area Prediction of Pennywort Plants Grown in a Plant Factory Using Image Processing and an Artificial Neural Network

Reza,

Chowdhury,

Islam

et al. 2023

Horticulturae

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The leaf is a primary part of a plant, and examining the leaf area is crucial in understanding growth and plant physiology. Accurately estimating leaf area is key to this understanding. This study proposed a methodology for the non-destructive estimation of leaf area in pennywort plants using image processing and an artificial neural network (ANN) model. The image processing method involved a series of steps, including grayscale conversion, histogram equalization, binary masking, and region filling, achieving an accuracy of around 96.6%. The ANN model, trained with 70% of a dataset, exhibited high correlations of 97.1% in training and 96.6% in testing phases, with leaf length and width significantly impacting the model output. A comparative analysis revealed the superior performance of the ANN model over the image processing method, demonstrating higher R2 values (>0.99) and lower errors. Furthermore, it showed the impact of diverse LED light combinations and nutrient levels (electrical conductivity, EC) on pennywort plant growth, indicating that the R70:B30 LED light ratio with nutrient level 2 (2.0 dS·m−1) fostered the most favorable growth for pennywort plants. The non-destructive nature, simplicity, and speed of the ANN model in estimating leaf area based on easily obtainable measurements of length and width render it an accessible and accurate tool for plant growth assessment in controlled environments. This approach offers opportunities for future studies, tracking changes in leaf areas under varied growth conditions without harming the plant, thus enhancing precision in research.

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“…Notable bioactive compounds of C. asiatica are triterpenoid saponins and sapogenins, among which the saponines madecassoside and asiaticoside are of particular interest, as well as their respective sapogenins madecassic and asiatic acid. Asiaticoside stimulates wound healing and is useful in the treatment of leprosy (Chakrabarty and Deshmukh 1976) and tuberculosis, while madecassoside has antiinflamatory properties and is able to increase collagen III secretion significantly (Solet et al 1986). As shown in Fig.…”

Section: Introductionmentioning

confidence: 98%

Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica

Mangas

Moyano

Osuna³

et al. 2008

Biotechnol Lett

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Centella asiatica has been extensively studied but there has been no report to date that relates gene expression and centelloside production in non-differentiated tissues. We have determined the content of the four principal triterpenoid bioactive compounds of C. asiatica (asiaticoside, madecassoside, asiatic acid and madecassic acid) in calli grown in different media and checked the expression level of some of the genes in the centelloside biosynthetic pathway. The results when compared with data from in vitro plant cultures showed a significantly lower expression of the gene encoding beta-amyrin synthase in calli, which is consistent with the observed lower production of centellosides (less than 900 microg/g DW), while in the plants the production was around 1.5-2 mg/g DW. Moreover, we find an efficient housekeeping gene for this plant. The biosynthesis of phytosterols is also discussed.

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Centella asiatica (L.) Urban, (Pennywort): Cell Culture, Production of Terpenoids, and Biotransformation Capacity

Cited by 3 publications

References 20 publications

Somatic Embryogenesis and Plant Development in Centella asiatica L., a Highly Prized Medicinal Plant of the Tropics

Somatic Embryogenesis and Plant Development in Centella asiatica L., a Highly Prized Medicinal Plant of the Tropics

Leaf Area Prediction of Pennywort Plants Grown in a Plant Factory Using Image Processing and an Artificial Neural Network

Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica

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