Centellaasiatica Morphotypes Differ Genetically as well as Macronutrients Content, Total Phenolic Content and Chemical Fingerprints of Leaves

Abstract

Purpose: Centellaassiatica is an aromatic herb commonly grown in humid areas of tropical Asia. It has widely been used as a leafy vegetable and for medicinal preparations. Its medicinal and dilatory values have created a high demand for fresh consumption and value added products. Assessing morphological, biochemical and genetic diversity of existing germplasm is the essential fi rst step in cultivation and breeding eff orts. Research Method:We analyzed leaf morphological characters, macro nutrient contents, total phenolic content and the high performance liquid chromatography (HPLC) profi les of fi ve most commonly found C. assiatica morphotypes in Sri Lanka. The genetic diversity was assessed using inter simple sequence repeats (ISSR) regions. All morphotypes were collected and grown under controlled environmental conditions.Findings: There were clear morphological diff erences among diff erent morphotypes. Further, there was a signifi cant diff erence (P<0.05) among them in leaf macro nutrient content and total phenolic contents. The ISSR profi les and the HPLC profi les of Heengotukola are distinctly diff erent from others. Originality/value: Results show the signifi cance of selecting superior morphotypes and for industrial applications for maintaining the standards.

Mitoprotective Effects of Centella asiatica (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease

“…Wide chemotypic variations in triterpenoids were found in CA planted in different growing regions, altitudes and localities ( Long et al, 2012 ; Singh et al, 2014 ; Srivastava et al, 2014 ). Genotypic and phenotypic variability have been associated with differences in phytochemicals content of CA including macronutrients, micronutrients, phenolics, flavonoids, tannin, anthocyanin, carotenoids and ascorbic acid ( Thomas et al, 2010 ; Singh et al, 2014 ; Lal et al, 2017 ; Chandrasekara et al, 2020 ). Other than geographical and genotypical influences, the phytochemical compositions of CA also vary due to seasonal variations associated with the cultivation and harvesting procedures, light conditions, as well as the drying conditions post-harvesting ( Maulidiani et al, 2012 ; Rahajanirina et al, 2012 ; Alqahtani et al, 2015 ; Plengmuankhae and Tantitadapitak, 2015 ).…”

Variability of Morphological and Agronomical Characteristics of Centella asiatica in Thailand

“…Cluster analysis based on morphologies and agronomic characteristics was studied in various plant species, such as pigeon pea, rice and soybean [20][21][22]. In Asiatic pennywort, Chandrasekara et al [17] was clustered the 14 morphologies of 5 morphotypes of Asiatic pennywort in Sri Lanka, the results revealed that 2 differed clusters were classified. Ravi et al [23] identified the variability of 39 accessions of Asiatic pennywort in India based on 14 morphological traits, the results shown wide variability.…”

“…According with, Prasad et al [11] reported that the variability of Asiatic pennywort germplasm collected from different part of India shown differed biomass accumulation and madecassoside and asiatic acid content. While, Chandrasekara et al [17] revealed that germplasm evaluation of Asiatic pennywort by using morphological, biochemical and genetic diversity is the essential 1 st step in cultivation and breeding efforts. Therefore, the objective of this study aimed to collected and identified the diversity of Asiatic pennywort in Thailand by using morphological and agronomical characteristic data.…”