Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation

Jing, Zhenli; Yin, Huilong; Wang, Pan; Gao, Juntao; Li, Yuan

doi:10.1016/j.bbrc.2016.02.079

Cited by 12 publications

(11 citation statements)

References 23 publications

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“…Titanium dioxide altered microtubular function . The highly up‐regulated lncRNA RP11‐132E11.2 or lnc‐CNTLN‐1 on chromosome 9 is associated with the CNTLN or centlein gene that encodes a centrosomal protein implicated in microtubule stability . Taken together, it may be hypothesized that titanium‐associated microtubular dysfunction may be a pathological mechanism in peri‐implantitis and under lncRNA regulation.…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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Background and Objective:Peri-implantitis is a biofilm-mediated infectious disease that results in progressive loss of implant-supporting bone. As compared to its analogue periodontitis, peri-implantitis is generally known to be more aggressive, with comparatively rapid progression and less predictable treatment outcomes, especially when advanced. An understanding of molecular mechanisms underpinning the similarities and differences between peri-implantitis and periodontitis is essential to develop novel management strategies. This study aimed to compare long non-coding RNAs (lncRNAs) and messenger RNA (mRNA) expression profiles between peri-implantitis and periodontitis.Methods: Inflamed soft tissue from peri-implantitis and periodontitis lesions, and healthy gingival tissue controls were analyzed by microarray. Cluster graphs, gene ontology (GO) analysis, and pathway analysis were performed. Quantitative real-time PCR was employed to verify microarray results. The expression levels of RANKL and OPG in the three tissue types were also evaluated, using qRT-PCR. Coding non-coding (CNC) network analyses were performed.Results: Microarray analyses revealed 1079 lncRNAs and 1003 mRNAs as differentially expressed in peri-implantitis when compared to periodontitis. The cyclooxygenase-2 pathway was the most up-regulated biological process in peri-implantitis as compared to periodontitis, whereas hemidesmosome assembly was the most down-regulated pathway. Osteoclast differentiation was relatively up-regulated, and RANKL/OPG ratio was higher in peri-implantitis than in periodontitis. Conclusions:The study demonstrated that peri-implantitis and periodontitis exhibit significantly different lncRNA and mRNA expression profiles, suggesting that osteoclast differentiation-related pathways are comparatively more active in periimplantitis. These data highlight potential molecular targets for periodontitis and peri-implantitis therapy development. K E Y W O R D SlncRNA, microarray, peri-implantitis, periodontitis, transcriptome | 343 LIU et aL.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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“…Centlein is a microtubule-associated protein that can directly bind to purified microtubules via its longest coiled-coil domain (34). The differential protein and gene expression levels of centlein in cancer tissues compared with adjacent tissues may be caused by the microtubule proteins.…”

Section: Discussionmentioning

confidence: 99%

Serum and tissue proteomic signatures of patients with hepatocellular carcinoma using 2‑D gel electrophoresis

et al. 2019

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Hepatocellular carcinoma (HCC) accounts for ~85% of primary liver cancer cases and is a leading cause of mortality worldwide. Effective early diagnosis is difficult for HCC; however, effective biomarkers may be beneficial for diagnosis. In the current study, serum samples, and HCC and adjacent tissue samples were obtained from patients with HCC for the detection of biomarkers using 2-D gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight (TOF)/TOF mass spectrometry. The crude serum samples did not need to be prepared for removal of high abundance proteins. The mRNA expression levels of HCC-associated proteins were detected in tissues using reverse transcription-quantitative PCR. Statistical analysis and database matching were used to identify the differentially expressed proteins detected in the serum and tissue groups. Immunohistochemistry (IHC) was performed to detect the expression of significant proteins in HCC and adjacent tissues. The results revealed ~800 protein spots on a 2-DE gel that were detected in serum samples, and 1,200 spots were identified in the tissue samples. The protein and mRNA expression levels of oxysterol binding protein-like 11 (OSBPL11) in HCC serum and tissue samples were consistent. Pathway analysis demonstrated that members of the apolipoprotein family, particularly apolipoprotein E (APOE), and RAS family members were closely associated in HCC, either directly or via ferratin heavy polypeptide 1. IHC results demonstrated that the APOE protein serves an important role in liver cancer development. The lysis buffer used in the current study was effective for serum protein separation in 2-DE sample preparation. In addition, the present study revealed that downregulated OSBPL11 may be a potential indicator for HCC, and the apolipoprotein family, particularly APOE, and the RAS family may cooperatively serve an important role.

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“…However, it is often impossible to localize an exact tubulin-binding site in proteins. For example, sites of MT binding in Hook3 (Walenta et al, 2001), stu2p (Al-Bassam et al, 2006), Centlein (Jing et al, 2016) proteins include more than 200 amino acid residues, which is significantly larger than the fulllength RPL22e with its 128 amino acid residues.…”

Section: Discussionmentioning

confidence: 99%

On the interaction of ribosomal protein RPL22e with microtubules

Chudinova

Brodsky

Nadezhdina

2019

Cell Biology International

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Microtubule (MT) protein preparations often contain components of the translation machinery, including ribosome proteins. To understand the biological meaning of it we studied the interaction of ribosomal protein RPL22e with the MT. We found that bacteria expressed purified RPL22e‐GFP‐6His did co‐sediment with brain tubulin MTs with 1.3 µM dissociation coefficient. Such a KD is comparable to some specific MT‐associated proteins. Distinct in vitro interaction of RPL22e‐GFP with MTs was also observed by TIRF microscopy. In real‐time assay, RPL22e‐GFP molecules stayed bound to MTs for several seconds, and 15% of them demonstrated random‐walk along MTs with diffusion coefficient 0.03 µ2/s. Deletion of basic areas of RPL22e did not have an impact on KD, and deletion of acidic tail slightly increased association with MTs. Interestingly, the deletion of acidic tail increased diffusion coefficient as well. The interaction of RPL22e with MTs is hardly noticeable in vivo in cultured cells, probably since a significant part of the protein is incorporated into the ribosomes. The mobility of ribosomal protein on the MTs probably prevents its interfering with MT‐dependent transport and could ameliorate its transport to the nucleus.

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Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation

Cited by 12 publications

References 23 publications

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Serum and tissue proteomic signatures of patients with hepatocellular carcinoma using 2‑D gel electrophoresis

On the interaction of ribosomal protein RPL22e with microtubules

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