Central administration of aminooxyacetate, an inhibitor of H2S production, affects thermoregulatory but not cardiovascular and ventilatory responses to hypercapnia in spontaneously hypertensive rats

Role of hydrogen sulfide in ventilatory responses to hypercapnia in the medullary raphe of adult rats

“…The study by Dias et al (2007) highlighted the participation of the MR in the ventilatory response to CO 2 by demonstrating that the Similarly, our previous studies (da Sabino et al, 2019) showed that inhibition of H 2 S production through the administration of AOA in the 4V caused a reduction in the ventilatory response to hypercapnia. More recently, it was demonstrated that endogenous H 2 S does play an excitatory role within the pre-Bötzinger complex, upmodulating basal inspiratory activity (in vitro/in vivo) and also the hypoxic ventilatory responses (in vivo) (da Silva et al, 2017).…”

“…Indeed, it is currently known that the CBS-H 2 S pathway is involved in neuropathic pain (Gui et al, 2016), hypertension (Huang et al, 2015) and carcinogenesis (Phillips et al, 2017). Recently, our group assessed the role of H 2 S in respiratory and temperature control, focusing on the ventilatory and thermoregulatory responses to either hypoxia (da Silva et al, 2017;Donatti et al, 2014;Kwiatkoski et al, 2012Kwiatkoski et al, , 2014Sabino et al, 2016Sabino et al, , 2017 or hypercapnia (da Silva et al, 2014;Sabino et al, 2019). The excitatory role of endogenous H 2 S was also described in the pre-Bötzinger complex, a brainstem site that is critically involved in respiratory rhythmogenesis (da Silva et al, 2017).…”

“…Hypercapnia can be defined as an increase in blood partial pressure of carbon dioxide (P CO 2 ), which stimulates both peripheral and central CO 2 /pH chemoreceptors, leading to reflex adjustments in the ventilatory pattern, that is, increased respiratory frequency (f R ), tidal volume (V T ), and ventilation (V E ) (Akilesh et al, 1997;da Silva et al, 2014;Sabino et al, 2019), as well as other physiological effects, for example, increased arterial blood pressure and decreased body temperature (Sabino et al, 2019…”

CBS‐Induced H2S Generation in Hippocampus Inhibits EA‐Induced Analgesia

“…Coordinates of A-P: 2.5 mm relative to the bregma, lateral: ±2.5 mm relative to the midline, and depth: 2 mm from the duramater were used for implanting guide cannulas (RWD, China) into the bilateral hippocampi [22]. After recovery from surgery, HA (9 nmol/1 μl [23]), AOAA (9 nmol/1 μl [24,25]), or SAM (10 pmol/1 μl [26]) was bilaterally microinjected into the hippocampi. DAO and CAT inhibitors followed the same procedure.…”

“…ermal Hypersensitivity. To study the relationship between CBS-induced H 2 S in the brain and thermal hypersensitivity, the CBS inhibitor HA (9 nmol) [23] or AOAA (9 nmol) [24,25] was bilaterally microinjected into the hippocampus. Moreover, the CBS-specific agonist SAM (10 pmol) [26] or saline was also bilaterally injected into the hippocampus one day after CFA injection, and CAT and DAO inhibitors [37] followed the same procedure (Figure 3(a)).…”