Central and peripheral expression sites of phoenixin-14 immunoreactivity in rats

Prinz, Philip; Scharner, Sophie; Friedrich, Tiemo; Schalla, Martha A.; Goebel-Stengel, Miriam; Rose, Matthias; Stengel, Andreas

doi:10.1016/j.bbrc.2017.09.048

Cited by 50 publications

(51 citation statements)

References 34 publications

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“…Surprisingly, a distinct assembly of phoenixin-expressing cells was recently found in the rat central amygdala (Prinz et al 2017 relationship between peripheral phoenixin levels and anxiety in a large group (68) of obese psychometrically diagnosed (GAD-7) male in-patients. The levels of depression and perceived stress were also measured using PHQ-9 and PSQ-20 scales respectively.…”

Section: Phoenixin In Autonomic and Mental Functionsmentioning

confidence: 99%

The potential role of the novel hypothalamic neuropeptides nesfatin-1, phoenixin, spexin and kisspeptin in the pathogenesis of anxiety and anorexia nervosa

Pałasz

Janas–Kozik

Borrow

et al. 2018

Neurochemistry International

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Due to the dynamic development of molecular neurobiology and bioinformatic methods several novel brain neuropeptides have been identified and characterized in recent years. Contemporary techniques of selective molecular detection e.g. in situ Real-Time PCR, microdiffusion and some bioinformatics strategies that base on searching for single structural features common to diverse neuropeptides such as hidden Markov model (HMM) have been successfully introduced. A convincing majority of neuropeptides have unique properties as well as a broad spectrum of physiological activity in numerous neuronal pathways including the hypothalamus and limbic system. The newly discovered but uncharacterized regulatory factors nesfatin-1, phoenixin, spexin and kisspeptin have the potential to be unique modulators of stress responses and eating behaviour. Accumulating basic studies revelaed an intriguing role of these neuropeptides in the brain pathways involved in the pathogenesis of anxiety behaviour. Nesfatin-1, phoenixin, spexin and kisspeptin may also distinctly affect the energy homeostasis and modulate food intake not only at the level of hypothalamic centres. Moreover, in patients suffered from anxiety and anorexia nervosa a significant, sex-related changes in the plasma neuropeptide levels occurred. It should be therefore taken into account that the targeted pharmacomodulation of central peptidergic signaling may be potentially helpful in the future treatment of certain neuropsychiatric and metabolic disorders. This article reviews recent evidence dealing with the hypothetical role of these new factors in the anxiety-related circuits and pathophysiology of anorexia nervosa.

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Section: Phoenixin In Autonomic and Mental Functionsmentioning

confidence: 99%

The potential role of the novel hypothalamic neuropeptides nesfatin-1, phoenixin, spexin and kisspeptin in the pathogenesis of anxiety and anorexia nervosa

Pałasz

Janas–Kozik

Borrow

et al. 2018

Neurochemistry International

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“…The discovery and tissue mapping of the novel peptide player in this regulatory game: phoenixin (PNX) and its receptor GPR173 shed a new light on HPG axis physiology. In the nervous system, the presence of PNX was identified in the limited neural populations in the hypothalamus, central amygdala, anterior horns of spinal cord, spinal trigeminal and solitary tracts and sensory ganglia . A peripheral PNX expression takes place in the gastrointestinal tract and ovary …”

Section: Discussionmentioning

confidence: 99%

“…PNX exists in two different, active molecular forms PNX‐14 and PNX‐20. PNX, derived from the proteolytic cleavage of the precursor Small Integral Membrane Protein 20 (SMIM20), is highly preserved across species and expressed in different tissues with strongest immunoreactivity in the hypothalamus, and with binding sites in the hypothalamic nuclei, pituitary gland, and ovary . The first experimental evidence for PNX involvement in reproduction was obtained in a female rat model through the use of small interfering RNA, able to prejudice the endogenous production of PNX, that resulted in an altered onset of oestrus and in decreased GnRH receptor mRNA expression.…”

Section: Introductionmentioning

confidence: 99%

The GnRH analogues affect novel neuropeptide SMIM20/phoenixin and GPR173 receptor expressions in the female rat hypothalamic–pituitary–gonadal (HPG) axis

Suszka-Świtek

Pałasz

Filipczyk

et al. 2019

Clin Exp Pharma Physio

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The recently discovered peptide phoenixin (PNX) and its receptor GPR173 are novel factors that exhibit a large spectrum of regulatory activity, especially when considered as a central modulator of GnRH-related hormonal control of reproductive processes. It has been already proven that GnRH agonists and antagonists can modulate peptidergic signalling in the HPG axis. Despite these findings, there is so far no information regarding the influence of treatment with GnRH analogues on SMIM20/phoenixin signalling in the hypothalamic-pituitary-gonadal axis. In the current study, SMIM20/ phoenixin and GPR173 mRNA levels were measured in the hypothalamus, pituitary and ovaries of female rats in the dioestrus phase following treatment with GnRH-R agonist (buserelin) and antagonist (cetrorelix) using quantitative real-time PCR. The serum PNX concentrations were also estimated with ELISA technique. The hypothalamic, hypophyseal and especially ovarian levels of SMIM20 mRNA were increased after both buserelin and cetrorelix administration. The GPR173 expressions were in turn decreased in the hypothalamus and pituitary. Treatment with the GnRH analogues led to the modulation of SMIM20/phoenixin and GPR173 mRNA expression in the female rat hypothalamic-pituitary-gonadal axis. By identifying buserelin and cetrorelix as novel modulators of phoenixin signalling in the animal HPG axis, these results cast new light on the GnRH analogues mode of action and contribute to a better understanding of the mechanisms responsible for the hormonal control of reproduction.

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“…1 More recently, Prinz et al investigated peripheral tissue expression of phoenixin-14 in male and female Sprague-Dawley rodents, describing a similar pattern to Yosten et al, but additionally reporting phoenixin activity in the amygdala, spinocerebellar tract, cells between the crypts of duodenum, jejunum and ileum, and peripheral parts of the islets of Langerhans in the pancreas. 5 While gonadal expression of phoenixin was not described in early male rodent studies, 1 binding sites in the ovaries were reported, and recent evidence has emerged of ovarian expression of the phoenixin precursor, SMIM20. 6…”

Section: Sites Of Phoenixin Expressionmentioning

confidence: 99%

Phoenixin and Its Role in Reproductive Hormone Release

Clarke

Dhillo

2019

Semin Reprod Med

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Phoenixin is novel neuropeptide, recently identified following the description of a peptide sequence highly conserved across several animal species, including humans, cows, and pigs. Expressed both centrally in the hypothalamus and arcuate nucleus and peripherally in cardiac and gastrointestinal tissue, it appears to modulate reproductive hormone secretion in a gonadotrophin-releasing hormone–dependent manner and may also influence anxiety and memory. While there remains much to be described regarding its signaling, this review assesses the currently available literature in both animal and human studies to summarize our understanding of the physiological role of this novel neuropeptide, and its function in reproductive hormone release.

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Central and peripheral expression sites of phoenixin-14 immunoreactivity in rats

Cited by 50 publications

References 34 publications

The potential role of the novel hypothalamic neuropeptides nesfatin-1, phoenixin, spexin and kisspeptin in the pathogenesis of anxiety and anorexia nervosa

The potential role of the novel hypothalamic neuropeptides nesfatin-1, phoenixin, spexin and kisspeptin in the pathogenesis of anxiety and anorexia nervosa

The GnRH analogues affect novel neuropeptide SMIM20/phoenixin and GPR173 receptor expressions in the female rat hypothalamic–pituitary–gonadal (HPG) axis

Phoenixin and Its Role in Reproductive Hormone Release

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