Central and peripheral region tibial plateau chondrocytes respond differently to in vitro dynamic compression

Bevill, Scott L.; Briant, Paul; Levenston, Marc E.; Andriacchi, Thomas P.

doi:10.1016/j.joca.2008.12.005

Cited by 47 publications

(41 citation statements)

References 70 publications

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“…Articular cartilage was obtained from the 36 stifle joints of healthy 6-8 month juvenile pigs within 4 h of sacrifice; the animals were sacrificed for an unrelated study. This animal model has been used in a previous study of the effect of mechanical loading on chondrocyte gene expression [16]. The joints were opened under aseptic conditions and rinsed periodically with phosphate-buffered saline (pH 7.2, Invitrogen, Carlsbad, CA) during sample harvesting.…”

Section: Methodsmentioning

confidence: 99%

“…Evidence for the support of a kinematic pathway has been based on evidence of regional topological variations in the properties of knee articular cartilage structure that are thought to arise in response to the variation in local mechanical environment [15]. It has been shown that chondrocyte populations in different regions of the tibial plateau (covered by meniscus versus not covered by meniscus) respond differently to equivalent tissue level mechanical loading [16]. Thus alteration in the in vivo functional joint motion may result in subtle shifts in the load distribution at the knee altering the local mechanical environment and exposing chondrocytes to loading patterns they are unaccustomed to thereby contributing to degenerative changes.…”

Section: Introductionmentioning

confidence: 99%

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The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli

Bevill

Boyer

Andriacchi

2014

Journal of Biomechanical Engineering

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Both mechanical load and elevated levels of proinflammatory cytokines have been associated with the risk for developing osteoarthritis (OA), yet the potential interaction of these mechanical and biological factors is not well understood. The purpose of this study was to evaluate the response of chondrocytes to the effects of dynamic unconfined compression, TNF-a, and the simultaneous effects of dynamic unconfined compression and TNF-a. The response to these three treatments was markedly different and, taken together, the response in the gene expression of chondrocytes to the different treatment conditions suggest a complex interaction between structure, biology, and mechanical loading.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli

Bevill

Boyer

Andriacchi

2014

Journal of Biomechanical Engineering

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“…Without in vivo data or in silico estimates of forces distributed across the articular surface of the pig humeral head, it is difficult to gauge the functional significance of regional variation in cartilage parameters. Interestingly, a recent study demonstrated the presence of regional differences in articular chondrocyte responses to mechanical loading, suggesting the possibility that even under uniform loads different cartilage regions may not respond uniformly (Bevill et al, 2009). As chondrocytes are particularly sensitive to the three-dimensional microenvironment and differentiate in response to local signals (Lemare et al, 1998;Goldring, 2004a;Goldring, 2004b), the expression of cartilage ECM elements likely exhibit regional variation due to subtle differences in loading patterns in distinct joint regions (Bayliss et al, 1983;Nakano and Scott, 1989;Mow et al, 1990;Mizoguchi et al, 1996;Tanaka et al, 2000).…”

Section: P<005 Limb Loading and Joint Plasticitymentioning

confidence: 99%

Differential limb loading in miniature pigs (Sus scrofa domesticus): a test of chondral modeling theory

Congdon

Hammond

Ravosa

2012

Journal of Experimental Biology

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SUMMARYVariation in mechanical loading is known to influence chondrogenesis during joint formation. However, the interaction among chondrocyte behavior and variation in activity patterns is incompletely understood, hindering our knowledge of limb ontogeny and function. Here, the role of endurance exercise in the development of articular and physeal cartilage in the humeral head was examined in 14 miniature swine (Sus scrofa domesticus). One group was subjected to graded treadmill running over a period of 17 weeks. A matched sedentary group was confined to individual pens. Hematoxylin and eosin staining was performed for histomorphometry of cartilage zone thickness, chondrocyte count and cell area, with these parameters compared multivariately between exercised and sedentary groups. Comparisons were also made with femora from the same sample, focusing on humerus-femur differences between exercised and sedentary groups, within-cohort comparisons of humerus-femur responses and correlated changes within and across joints. This study shows conflicting support for the chondral modeling theory. The humeral articular cartilage of exercised pigs was thinner than that of sedentary pigs, but their physeal cartilage was thicker. While articular and physeal cartilage demonstrated between-cohort differences, humeral physeal cartilage exhibited load-induced responses of greater magnitude than humeral articular cartilage. Controlling for cohort, the humerus showed increased chondrocyte mitosis and cell area, presumably due to relatively greater loading than the femur. This represents the first known effort to evaluate chondral modeling across multiple joints from the same individuals. Our findings suggest the chondral response to elevated loading is complex, varying within and among joints. This has important implications for understanding joint biomechanics and development.

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“…It was recently shown that asymptomatic knees have a characteristic region of thicker cartilage in each of the medial, lateral, and trochlea compartments [20][21][22]. These regions are of primary interest in a first analysis of the cartilage thickness shape because articular cartilage thickness and metabolism have been shown to locally adapt in response to applied loads [2,[23][24][25], knee loading has been reported to change with OA progression [26][27][28][29], and OA-related cartilage lesions have been associated with areas of high mechanical stress [30]. These three regions also agree with typical injury sites and the location of full-thickness lesions in the femoral cartilage [31,32], further supporting their selection for this study.…”

Section: Introductionmentioning

confidence: 99%

Patterns of Femoral Cartilage Thickness are Different in Asymptomatic and Osteoarthritic Knees and Can be Used to Detect Disease-Related Differences Between Samples

Favre¹,

Scanlan

Erhart‐Hledik³

et al. 2013

Journal of Biomechanical Engineering

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Measures of mean cartilage thickness over predefined regions in the femoral plate using magnetic resonance imaging have provided important insights into the characteristics of knee osteoarthritis (OA), however, this quantification method suffers from the limited ability to detect OA-related differences between knees and loses potentially important information regarding spatial variations in cartilage thickness. The objectives of this study were to develop a new method for analyzing patterns of femoral cartilage thickness and to test the following hypotheses: (1) asymptomatic knees have similar thickness patterns, (2) thickness patterns differ with knee OA, and (3) thickness patterns are more sensitive than mean thicknesses to differences between OA conditions. Bi-orthogonal thickness patterns were extracted from thickness maps of segmented magnetic resonance images in the medial, lateral, and trochlea compartments. Fifty asymptomatic knees were used to develop the method and establish reference asymptomatic patterns. Another subgroup of 20 asymptomatic knees and three subgroups of 20 OA knees each with a Kellgren/Lawrence grade (KLG) of 1, 2, and 3, respectively, were selected for hypotheses testing. The thickness patterns were similar between asymptomatic knees (coefficient of multiple determination between 0.8 and 0.9). The thickness pattern alterations, i.e., the differences between the thickness patterns of an individual knee and reference asymptomatic thickness patterns, increased with increasing OA severity (Kendall correlation between 0.23 and 0.47) and KLG 2 and 3 knees had significantly larger thickness pattern alterations than asymptomatic knees in the three compartments. On average, the number of significant differences detected between the four subgroups was 4.5 times greater with thickness pattern alterations than mean thicknesses. The increase was particularly marked in the medial compartment, where the number of significant differences between subgroups was 10 times greater with thickness pattern alterations than mean thickness measurements. Asymptomatic knees had characteristic regional thickness patterns and these patterns were different in medial OA knees. Assessing the thickness patterns, which account for the spatial variations in cartilage thickness and capture both cartilage thinning and swelling, could enhance the capacity to detect OA-related differences between knees.

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Central and peripheral region tibial plateau chondrocytes respond differently to in vitro dynamic compression

Cited by 47 publications

References 70 publications

The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli

The Regional Sensitivity of Chondrocyte Gene Expression to Coactive Mechanical Load and Exogenous TNF-α Stimuli

Differential limb loading in miniature pigs (Sus scrofa domesticus): a test of chondral modeling theory

Patterns of Femoral Cartilage Thickness are Different in Asymptomatic and Osteoarthritic Knees and Can be Used to Detect Disease-Related Differences Between Samples

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